Experiment: MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU1261 and pilT-1 are separated by 8 nucleotides pilT-1 and pppA are separated by 175 nucleotides
DVU1261: DVU1261 - conserved domain protein (TIGR), at 1,348,254 to 1,350,014
DVU1261
DVU1262: pilT-1 - twitching motility protein PilT (TIGR), at 1,350,023 to 1,351,132
pilT-1
DVU1263: pppA - type IV prepilin-like proteins leader peptidase (TIGR), at 1,351,308 to 1,352,111
pppA
Position (kb)
1350
1351
1352 Strain fitness (log2 ratio)
-1
0
1
2 at 1349.142 kb on - strand, within DVU1261 at 1349.226 kb on - strand, within DVU1261 at 1349.226 kb on - strand, within DVU1261 at 1349.360 kb on + strand, within DVU1261 at 1349.365 kb on + strand, within DVU1261 at 1349.379 kb on + strand, within DVU1261 at 1349.388 kb on - strand, within DVU1261 at 1349.419 kb on + strand, within DVU1261 at 1349.489 kb on - strand, within DVU1261 at 1349.592 kb on - strand, within DVU1261 at 1349.592 kb on - strand, within DVU1261 at 1349.779 kb on - strand, within DVU1261 at 1349.890 kb on - strand at 1349.905 kb on - strand at 1349.917 kb on - strand at 1350.072 kb on + strand at 1350.331 kb on + strand, within pilT-1 at 1350.534 kb on + strand, within pilT-1 at 1350.620 kb on + strand, within pilT-1 at 1350.759 kb on - strand, within pilT-1 at 1350.920 kb on + strand, within pilT-1 at 1350.922 kb on - strand, within pilT-1 at 1350.972 kb on - strand, within pilT-1 at 1350.979 kb on + strand, within pilT-1 at 1351.014 kb on + strand, within pilT-1 at 1351.058 kb on + strand at 1351.065 kb on + strand at 1351.073 kb on + strand at 1351.139 kb on + strand at 1351.147 kb on - strand at 1351.183 kb on - strand at 1351.209 kb on + strand at 1351.269 kb on - strand at 1351.329 kb on - strand at 1351.527 kb on + strand, within pppA at 1351.541 kb on + strand, within pppA at 1351.615 kb on - strand, within pppA at 1351.632 kb on + strand, within pppA at 1351.790 kb on - strand, within pppA at 1351.790 kb on - strand, within pppA at 1351.839 kb on + strand, within pppA at 1351.851 kb on + strand, within pppA at 1351.861 kb on - strand, within pppA at 1351.877 kb on + strand, within pppA
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05% remove 1,349,142 - DVU1261 0.50 +2.0 1,349,226 - DVU1261 0.55 +0.4 1,349,226 - DVU1261 0.55 -0.2 1,349,360 + DVU1261 0.63 +1.0 1,349,365 + DVU1261 0.63 -0.2 1,349,379 + DVU1261 0.64 -0.4 1,349,388 - DVU1261 0.64 +0.1 1,349,419 + DVU1261 0.66 -0.3 1,349,489 - DVU1261 0.70 +1.6 1,349,592 - DVU1261 0.76 +0.3 1,349,592 - DVU1261 0.76 -0.2 1,349,779 - DVU1261 0.87 -0.8 1,349,890 - +1.0 1,349,905 - +0.0 1,349,917 - +0.5 1,350,072 + +0.2 1,350,331 + pilT-1 DVU1262 0.28 +0.1 1,350,534 + pilT-1 DVU1262 0.46 -0.6 1,350,620 + pilT-1 DVU1262 0.54 +1.0 1,350,759 - pilT-1 DVU1262 0.66 +1.2 1,350,920 + pilT-1 DVU1262 0.81 -0.3 1,350,922 - pilT-1 DVU1262 0.81 +0.0 1,350,972 - pilT-1 DVU1262 0.85 +0.5 1,350,979 + pilT-1 DVU1262 0.86 -0.2 1,351,014 + pilT-1 DVU1262 0.89 -0.7 1,351,058 + -0.6 1,351,065 + +0.0 1,351,073 + -0.1 1,351,139 + -0.2 1,351,147 - -0.3 1,351,183 - -0.2 1,351,209 + -0.4 1,351,269 - -0.5 1,351,329 - +0.0 1,351,527 + pppA DVU1263 0.27 +0.4 1,351,541 + pppA DVU1263 0.29 -0.0 1,351,615 - pppA DVU1263 0.38 +0.8 1,351,632 + pppA DVU1263 0.40 -1.5 1,351,790 - pppA DVU1263 0.60 -0.2 1,351,790 - pppA DVU1263 0.60 +0.7 1,351,839 + pppA DVU1263 0.66 -0.5 1,351,851 + pppA DVU1263 0.68 -0.0 1,351,861 - pppA DVU1263 0.69 -1.7 1,351,877 + pppA DVU1263 0.71 +0.6
Or see this region's nucleotide sequence