Experiment: MoYLS4 with Polyethylene glycol 5%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyrF and DVU0902 are separated by 132 nucleotides DVU0902 and DVU0903 are separated by 4 nucleotides DVU0903 and recJ are separated by 56 nucleotides
DVU0901: pyrF - orotidine 5`-phosphate decarboxylase (TIGR), at 993,049 to 993,756
pyrF
DVU0902: DVU0902 - TPR domain protein (TIGR), at 993,889 to 994,746
DVU0902
DVU0903: DVU0903 - HD domain protein (TIGR), at 994,751 to 995,608
DVU0903
DVU0904: recJ - single-stranded-DNA-specific exonuclease RecJ (TIGR), at 995,665 to 997,380
recJ
Position (kb) 994
995
996 Strain fitness (log2 ratio) -2
-1
0
1
2 at 993.803 kb on + strand at 993.804 kb on + strand at 993.895 kb on - strand at 994.007 kb on - strand, within DVU0902 at 994.047 kb on - strand, within DVU0902 at 994.074 kb on - strand, within DVU0902 at 994.247 kb on - strand, within DVU0902 at 994.435 kb on - strand, within DVU0902 at 994.435 kb on - strand, within DVU0902 at 994.502 kb on - strand, within DVU0902 at 994.518 kb on + strand, within DVU0902 at 994.518 kb on + strand, within DVU0902 at 994.518 kb on - strand, within DVU0902 at 994.526 kb on - strand, within DVU0902 at 994.635 kb on + strand, within DVU0902 at 994.663 kb on + strand at 994.709 kb on - strand at 994.711 kb on + strand at 994.720 kb on + strand at 994.725 kb on - strand at 994.743 kb on - strand at 994.840 kb on - strand, within DVU0903 at 994.840 kb on - strand, within DVU0903 at 995.099 kb on + strand, within DVU0903 at 995.112 kb on - strand, within DVU0903 at 995.167 kb on + strand, within DVU0903 at 995.167 kb on + strand, within DVU0903 at 995.175 kb on - strand, within DVU0903 at 995.175 kb on - strand, within DVU0903 at 995.223 kb on + strand, within DVU0903 at 995.223 kb on + strand, within DVU0903 at 995.231 kb on - strand, within DVU0903 at 995.269 kb on + strand, within DVU0903 at 995.283 kb on + strand, within DVU0903 at 995.325 kb on + strand, within DVU0903 at 995.338 kb on - strand, within DVU0903 at 995.400 kb on + strand, within DVU0903 at 995.541 kb on - strand at 995.548 kb on + strand at 995.593 kb on - strand at 995.627 kb on - strand at 995.634 kb on - strand at 995.683 kb on - strand at 995.961 kb on - strand, within recJ at 996.290 kb on - strand, within recJ at 996.334 kb on + strand, within recJ at 996.342 kb on - strand, within recJ at 996.385 kb on + strand, within recJ at 996.393 kb on - strand, within recJ at 996.513 kb on + strand, within recJ
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polyethylene glycol 5% remove 993,803 + +0.1 993,804 + +0.8 993,895 - -1.1 994,007 - DVU0902 0.14 -1.7 994,047 - DVU0902 0.18 -0.2 994,074 - DVU0902 0.22 -0.2 994,247 - DVU0902 0.42 -0.2 994,435 - DVU0902 0.64 -0.9 994,435 - DVU0902 0.64 -0.2 994,502 - DVU0902 0.71 -0.6 994,518 + DVU0902 0.73 +0.4 994,518 + DVU0902 0.73 +0.1 994,518 - DVU0902 0.73 -2.0 994,526 - DVU0902 0.74 +0.0 994,635 + DVU0902 0.87 -0.1 994,663 + -0.4 994,709 - -0.1 994,711 + +0.0 994,720 + -0.1 994,725 - +0.8 994,743 - -0.4 994,840 - DVU0903 0.10 -0.2 994,840 - DVU0903 0.10 +0.2 995,099 + DVU0903 0.41 +1.2 995,112 - DVU0903 0.42 +0.2 995,167 + DVU0903 0.48 +1.6 995,167 + DVU0903 0.48 +0.5 995,175 - DVU0903 0.49 +0.5 995,175 - DVU0903 0.49 -0.3 995,223 + DVU0903 0.55 +1.8 995,223 + DVU0903 0.55 -0.3 995,231 - DVU0903 0.56 -0.3 995,269 + DVU0903 0.60 -0.0 995,283 + DVU0903 0.62 -0.0 995,325 + DVU0903 0.67 -1.3 995,338 - DVU0903 0.68 +0.1 995,400 + DVU0903 0.76 -0.0 995,541 - +0.8 995,548 + +1.4 995,593 - -0.0 995,627 - -0.5 995,634 - -0.6 995,683 - -0.7 995,961 - recJ DVU0904 0.17 +0.6 996,290 - recJ DVU0904 0.36 +1.4 996,334 + recJ DVU0904 0.39 +2.4 996,342 - recJ DVU0904 0.39 +0.4 996,385 + recJ DVU0904 0.42 +1.0 996,393 - recJ DVU0904 0.42 +0.0 996,513 + recJ DVU0904 0.49 +0.4
Or see this region's nucleotide sequence