Experiment: R2A with Vogels_fungal_media 0.2X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS18885 and HSERO_RS18890 are separated by 74 nucleotides HSERO_RS18890 and HSERO_RS18895 are separated by 159 nucleotides HSERO_RS18895 and HSERO_RS18900 are separated by 77 nucleotides
HSERO_RS18885: HSERO_RS18885 - gamma-glutamyltransferase, at 4,335,109 to 4,336,692
_RS18885
HSERO_RS18890: HSERO_RS18890 - hypothetical protein, at 4,336,767 to 4,337,312
_RS18890
HSERO_RS18895: HSERO_RS18895 - alkane 1-monooxygenase, at 4,337,472 to 4,338,482
_RS18895
HSERO_RS18900: HSERO_RS18900 - 2-alkenal reductase, at 4,338,560 to 4,339,591
_RS18900
Position (kb) 4337
4338
4339 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4336.488 kb on + strand, within HSERO_RS18885 at 4336.547 kb on + strand at 4336.555 kb on - strand at 4336.715 kb on - strand at 4336.825 kb on - strand, within HSERO_RS18890 at 4337.073 kb on + strand, within HSERO_RS18890 at 4337.081 kb on - strand, within HSERO_RS18890 at 4337.102 kb on - strand, within HSERO_RS18890 at 4337.228 kb on + strand, within HSERO_RS18890 at 4337.313 kb on + strand at 4337.321 kb on - strand at 4337.330 kb on - strand at 4337.360 kb on - strand at 4337.991 kb on + strand, within HSERO_RS18895 at 4338.151 kb on - strand, within HSERO_RS18895 at 4338.151 kb on - strand, within HSERO_RS18895 at 4338.205 kb on - strand, within HSERO_RS18895 at 4338.414 kb on + strand at 4338.438 kb on + strand at 4338.726 kb on - strand, within HSERO_RS18900 at 4338.783 kb on - strand, within HSERO_RS18900 at 4339.004 kb on + strand, within HSERO_RS18900 at 4339.039 kb on - strand, within HSERO_RS18900 at 4339.039 kb on - strand, within HSERO_RS18900 at 4339.176 kb on - strand, within HSERO_RS18900 at 4339.176 kb on - strand, within HSERO_RS18900 at 4339.429 kb on + strand, within HSERO_RS18900 at 4339.480 kb on - strand, within HSERO_RS18900
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.2X remove 4,336,488 + HSERO_RS18885 0.87 +0.2 4,336,547 + -3.4 4,336,555 - +0.4 4,336,715 - +0.1 4,336,825 - HSERO_RS18890 0.11 +0.6 4,337,073 + HSERO_RS18890 0.56 +0.3 4,337,081 - HSERO_RS18890 0.58 -0.1 4,337,102 - HSERO_RS18890 0.61 +0.6 4,337,228 + HSERO_RS18890 0.84 -0.1 4,337,313 + +0.1 4,337,321 - -0.2 4,337,330 - -0.1 4,337,360 - +1.7 4,337,991 + HSERO_RS18895 0.51 -0.3 4,338,151 - HSERO_RS18895 0.67 -1.1 4,338,151 - HSERO_RS18895 0.67 -0.8 4,338,205 - HSERO_RS18895 0.73 -0.0 4,338,414 + -0.0 4,338,438 + +0.9 4,338,726 - HSERO_RS18900 0.16 -0.0 4,338,783 - HSERO_RS18900 0.22 +0.0 4,339,004 + HSERO_RS18900 0.43 +0.2 4,339,039 - HSERO_RS18900 0.46 +2.0 4,339,039 - HSERO_RS18900 0.46 -1.0 4,339,176 - HSERO_RS18900 0.60 -0.2 4,339,176 - HSERO_RS18900 0.60 -0.4 4,339,429 + HSERO_RS18900 0.84 +1.3 4,339,480 - HSERO_RS18900 0.89 +0.5
Or see this region's nucleotide sequence