Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2618 and Psyr_2619 are separated by 214 nucleotides Psyr_2619 and Psyr_2620 are separated by 276 nucleotides
Psyr_2618: Psyr_2618 - ABC transporter:Protein of unknown function DUF214, at 3,173,564 to 3,175,525
_2618
Psyr_2619: Psyr_2619 - diaminobutyrate aminotransferase apoenzyme, at 3,175,740 to 3,177,074
_2619
Psyr_2620: Psyr_2620 - RND efflux system, outer membrane lipoprotein, NodT, at 3,177,351 to 3,178,781
_2620
Position (kb)
3175
3176
3177
3178 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3175.091 kb on + strand, within Psyr_2618 at 3175.091 kb on + strand, within Psyr_2618 at 3175.091 kb on + strand, within Psyr_2618 at 3175.092 kb on - strand, within Psyr_2618 at 3175.365 kb on - strand at 3175.631 kb on + strand at 3175.632 kb on - strand at 3175.634 kb on + strand at 3175.634 kb on + strand at 3175.634 kb on + strand at 3175.635 kb on - strand at 3175.635 kb on - strand at 3175.635 kb on - strand at 3175.779 kb on - strand at 3176.118 kb on - strand, within Psyr_2619 at 3176.199 kb on - strand, within Psyr_2619 at 3176.490 kb on + strand, within Psyr_2619 at 3176.503 kb on + strand, within Psyr_2619 at 3176.503 kb on + strand, within Psyr_2619 at 3176.525 kb on + strand, within Psyr_2619 at 3176.526 kb on - strand, within Psyr_2619 at 3176.953 kb on + strand at 3176.953 kb on + strand at 3177.025 kb on + strand at 3177.075 kb on - strand at 3177.247 kb on - strand at 3177.350 kb on + strand at 3177.350 kb on + strand at 3177.350 kb on + strand at 3177.350 kb on + strand at 3177.350 kb on + strand at 3177.351 kb on - strand at 3177.351 kb on - strand at 3177.351 kb on - strand at 3177.356 kb on + strand at 3177.419 kb on + strand at 3177.419 kb on + strand at 3177.419 kb on + strand at 3177.420 kb on - strand at 3177.420 kb on - strand at 3177.424 kb on + strand at 3178.031 kb on + strand, within Psyr_2620
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 3,175,091 + Psyr_2618 0.78 +0.7 3,175,091 + Psyr_2618 0.78 +1.6 3,175,091 + Psyr_2618 0.78 +0.6 3,175,092 - Psyr_2618 0.78 +0.2 3,175,365 - -0.1 3,175,631 + -0.8 3,175,632 - -0.9 3,175,634 + +0.8 3,175,634 + +1.2 3,175,634 + +0.7 3,175,635 - -1.9 3,175,635 - -0.9 3,175,635 - -0.7 3,175,779 - -0.5 3,176,118 - Psyr_2619 0.28 -0.2 3,176,199 - Psyr_2619 0.34 +0.1 3,176,490 + Psyr_2619 0.56 -0.5 3,176,503 + Psyr_2619 0.57 -1.1 3,176,503 + Psyr_2619 0.57 -0.6 3,176,525 + Psyr_2619 0.59 +0.0 3,176,526 - Psyr_2619 0.59 -0.0 3,176,953 + -1.5 3,176,953 + +0.3 3,177,025 + -0.1 3,177,075 - +0.0 3,177,247 - -0.9 3,177,350 + -0.4 3,177,350 + -2.4 3,177,350 + -3.1 3,177,350 + -1.3 3,177,350 + +0.0 3,177,351 - +0.2 3,177,351 - -2.2 3,177,351 - +0.9 3,177,356 + +1.8 3,177,419 + +1.2 3,177,419 + +1.8 3,177,419 + -0.2 3,177,420 - +1.0 3,177,420 - +1.2 3,177,424 + +0.4 3,178,031 + Psyr_2620 0.48 -0.3
Or see this region's nucleotide sequence