Experiment: KB with Novobiocin 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0339 and Psyr_0340 are separated by 38 nucleotides Psyr_0340 and Psyr_0341 are separated by 625 nucleotides Psyr_0341 and Psyr_0342 are separated by 89 nucleotides
Psyr_0339: Psyr_0339 - Binding-protein-dependent transport systems inner membrane component, at 361,263 to 362,198
_0339
Psyr_0340: Psyr_0340 - ABC transporter, periplasmic substrate-binding protein, at 362,237 to 363,256
_0340
Psyr_0341: Psyr_0341 - hypothetical protein, at 363,882 to 364,115
_0341
Psyr_0342: Psyr_0342 - hypothetical protein, at 364,205 to 365,200
_0342
Position (kb)
362
363
364 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 361.253 kb on + strand at 361.253 kb on + strand at 361.258 kb on - strand at 361.273 kb on + strand at 361.274 kb on - strand at 361.274 kb on - strand at 361.276 kb on + strand at 361.276 kb on + strand at 361.276 kb on + strand at 361.277 kb on - strand at 361.277 kb on - strand at 361.298 kb on + strand at 361.298 kb on + strand at 361.298 kb on + strand at 361.298 kb on + strand at 361.298 kb on + strand at 361.300 kb on + strand at 361.300 kb on + strand at 361.300 kb on + strand at 361.363 kb on - strand, within Psyr_0339 at 361.558 kb on - strand, within Psyr_0339 at 361.558 kb on - strand, within Psyr_0339 at 361.626 kb on + strand, within Psyr_0339 at 361.747 kb on + strand, within Psyr_0339 at 361.835 kb on + strand, within Psyr_0339 at 361.835 kb on + strand, within Psyr_0339 at 361.836 kb on - strand, within Psyr_0339 at 361.836 kb on - strand, within Psyr_0339 at 361.836 kb on - strand, within Psyr_0339 at 361.836 kb on - strand, within Psyr_0339 at 361.839 kb on + strand, within Psyr_0339 at 361.840 kb on - strand, within Psyr_0339 at 361.890 kb on + strand, within Psyr_0339 at 361.890 kb on + strand, within Psyr_0339 at 361.890 kb on + strand, within Psyr_0339 at 361.890 kb on + strand, within Psyr_0339 at 361.890 kb on + strand, within Psyr_0339 at 361.890 kb on + strand, within Psyr_0339 at 361.890 kb on + strand, within Psyr_0339 at 361.890 kb on + strand, within Psyr_0339 at 361.890 kb on + strand, within Psyr_0339 at 361.890 kb on + strand, within Psyr_0339 at 361.890 kb on + strand, within Psyr_0339 at 361.890 kb on + strand, within Psyr_0339 at 361.890 kb on + strand, within Psyr_0339 at 361.890 kb on + strand, within Psyr_0339 at 361.891 kb on - strand, within Psyr_0339 at 361.891 kb on - strand, within Psyr_0339 at 361.891 kb on - strand, within Psyr_0339 at 361.891 kb on - strand, within Psyr_0339 at 361.891 kb on - strand, within Psyr_0339 at 361.891 kb on - strand, within Psyr_0339 at 361.891 kb on - strand, within Psyr_0339 at 361.891 kb on - strand, within Psyr_0339 at 362.052 kb on + strand, within Psyr_0339 at 362.052 kb on + strand, within Psyr_0339 at 362.052 kb on + strand, within Psyr_0339 at 362.052 kb on + strand, within Psyr_0339 at 362.052 kb on + strand, within Psyr_0339 at 362.053 kb on - strand, within Psyr_0339 at 362.053 kb on - strand, within Psyr_0339 at 362.053 kb on - strand, within Psyr_0339 at 362.053 kb on - strand, within Psyr_0339 at 362.079 kb on + strand, within Psyr_0339 at 362.079 kb on + strand, within Psyr_0339 at 362.079 kb on + strand, within Psyr_0339 at 362.079 kb on + strand, within Psyr_0339 at 362.079 kb on + strand, within Psyr_0339 at 362.079 kb on + strand, within Psyr_0339 at 362.079 kb on + strand, within Psyr_0339 at 362.079 kb on + strand, within Psyr_0339 at 362.080 kb on - strand, within Psyr_0339 at 362.080 kb on - strand, within Psyr_0339 at 362.080 kb on - strand, within Psyr_0339 at 362.080 kb on - strand, within Psyr_0339 at 362.080 kb on - strand, within Psyr_0339 at 362.080 kb on - strand, within Psyr_0339 at 362.080 kb on - strand, within Psyr_0339 at 362.218 kb on + strand at 362.606 kb on + strand, within Psyr_0340 at 362.606 kb on + strand, within Psyr_0340 at 362.606 kb on + strand, within Psyr_0340 at 362.607 kb on - strand, within Psyr_0340 at 362.607 kb on - strand, within Psyr_0340 at 362.607 kb on - strand, within Psyr_0340 at 362.607 kb on - strand, within Psyr_0340 at 362.607 kb on - strand, within Psyr_0340 at 362.747 kb on + strand, within Psyr_0340 at 362.747 kb on + strand, within Psyr_0340 at 362.747 kb on + strand, within Psyr_0340 at 362.747 kb on + strand, within Psyr_0340 at 362.748 kb on - strand, within Psyr_0340 at 362.748 kb on - strand, within Psyr_0340 at 362.748 kb on - strand, within Psyr_0340 at 362.748 kb on - strand, within Psyr_0340 at 362.748 kb on - strand, within Psyr_0340 at 362.887 kb on + strand, within Psyr_0340 at 362.887 kb on + strand, within Psyr_0340 at 362.887 kb on + strand, within Psyr_0340 at 362.887 kb on + strand, within Psyr_0340 at 362.887 kb on + strand, within Psyr_0340 at 362.887 kb on + strand, within Psyr_0340 at 362.888 kb on - strand, within Psyr_0340 at 362.888 kb on - strand, within Psyr_0340 at 362.888 kb on - strand, within Psyr_0340 at 362.903 kb on + strand, within Psyr_0340 at 362.903 kb on + strand, within Psyr_0340 at 362.903 kb on + strand, within Psyr_0340 at 362.904 kb on - strand, within Psyr_0340 at 362.904 kb on - strand, within Psyr_0340 at 362.942 kb on + strand, within Psyr_0340 at 362.942 kb on + strand, within Psyr_0340 at 362.942 kb on + strand, within Psyr_0340 at 362.943 kb on - strand, within Psyr_0340 at 362.943 kb on - strand, within Psyr_0340 at 362.943 kb on - strand, within Psyr_0340 at 362.943 kb on - strand, within Psyr_0340 at 362.943 kb on - strand, within Psyr_0340 at 362.943 kb on - strand, within Psyr_0340 at 362.981 kb on + strand, within Psyr_0340 at 362.981 kb on + strand, within Psyr_0340 at 362.981 kb on + strand, within Psyr_0340 at 362.982 kb on - strand, within Psyr_0340 at 362.982 kb on - strand, within Psyr_0340 at 362.993 kb on + strand, within Psyr_0340 at 362.993 kb on + strand, within Psyr_0340 at 362.993 kb on + strand, within Psyr_0340 at 362.994 kb on - strand, within Psyr_0340 at 362.994 kb on - strand, within Psyr_0340 at 363.056 kb on + strand, within Psyr_0340 at 363.056 kb on + strand, within Psyr_0340 at 363.056 kb on + strand, within Psyr_0340 at 363.056 kb on + strand, within Psyr_0340 at 363.056 kb on + strand, within Psyr_0340 at 363.056 kb on + strand, within Psyr_0340 at 363.056 kb on + strand, within Psyr_0340 at 363.057 kb on - strand, within Psyr_0340 at 363.057 kb on - strand, within Psyr_0340 at 363.057 kb on - strand, within Psyr_0340 at 363.057 kb on - strand, within Psyr_0340 at 363.057 kb on - strand, within Psyr_0340 at 363.159 kb on - strand at 363.159 kb on - strand at 363.323 kb on + strand at 363.323 kb on + strand at 363.323 kb on + strand at 363.323 kb on + strand at 363.324 kb on - strand at 363.324 kb on - strand at 363.348 kb on - strand at 363.401 kb on + strand at 363.401 kb on + strand at 363.401 kb on + strand at 363.401 kb on + strand at 363.402 kb on - strand at 363.452 kb on + strand at 363.453 kb on - strand at 363.453 kb on - strand at 363.453 kb on - strand at 363.453 kb on - strand at 363.453 kb on - strand at 363.453 kb on - strand at 363.453 kb on - strand at 363.544 kb on - strand at 363.606 kb on + strand at 363.606 kb on + strand at 363.607 kb on - strand at 363.658 kb on + strand at 363.658 kb on + strand at 363.658 kb on + strand at 363.658 kb on + strand at 363.659 kb on - strand at 363.659 kb on - strand at 363.659 kb on - strand at 363.659 kb on - strand at 363.659 kb on - strand at 363.839 kb on - strand at 363.869 kb on - strand at 363.869 kb on - strand at 363.876 kb on + strand at 363.877 kb on - strand at 363.952 kb on + strand, within Psyr_0341 at 363.952 kb on + strand, within Psyr_0341 at 363.952 kb on + strand, within Psyr_0341 at 363.952 kb on + strand, within Psyr_0341 at 363.953 kb on - strand, within Psyr_0341 at 363.953 kb on - strand, within Psyr_0341 at 363.953 kb on - strand, within Psyr_0341 at 363.953 kb on - strand, within Psyr_0341 at 363.953 kb on - strand, within Psyr_0341 at 363.979 kb on - strand, within Psyr_0341 at 364.113 kb on + strand at 364.113 kb on + strand at 364.122 kb on + strand at 364.122 kb on + strand at 364.122 kb on + strand at 364.122 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Novobiocin 250 ug/ml remove 361,253 + -2.5 361,253 + -0.4 361,258 - -0.7 361,273 + -0.3 361,274 - +0.0 361,274 - +0.4 361,276 + +0.4 361,276 + -0.0 361,276 + +0.3 361,277 - +0.7 361,277 - -1.0 361,298 + +0.2 361,298 + +0.9 361,298 + -1.3 361,298 + -0.2 361,298 + -0.8 361,300 + +0.1 361,300 + +1.0 361,300 + -0.2 361,363 - Psyr_0339 0.11 -2.6 361,558 - Psyr_0339 0.32 -0.6 361,558 - Psyr_0339 0.32 +0.3 361,626 + Psyr_0339 0.39 +1.2 361,747 + Psyr_0339 0.52 -0.4 361,835 + Psyr_0339 0.61 +0.1 361,835 + Psyr_0339 0.61 +0.3 361,836 - Psyr_0339 0.61 -0.1 361,836 - Psyr_0339 0.61 -0.6 361,836 - Psyr_0339 0.61 +0.3 361,836 - Psyr_0339 0.61 -1.3 361,839 + Psyr_0339 0.62 -3.1 361,840 - Psyr_0339 0.62 -0.5 361,890 + Psyr_0339 0.67 +0.9 361,890 + Psyr_0339 0.67 +0.4 361,890 + Psyr_0339 0.67 -0.5 361,890 + Psyr_0339 0.67 +1.2 361,890 + Psyr_0339 0.67 -1.5 361,890 + Psyr_0339 0.67 -0.8 361,890 + Psyr_0339 0.67 +3.2 361,890 + Psyr_0339 0.67 +1.2 361,890 + Psyr_0339 0.67 +1.7 361,890 + Psyr_0339 0.67 +0.6 361,890 + Psyr_0339 0.67 -0.9 361,890 + Psyr_0339 0.67 +0.3 361,890 + Psyr_0339 0.67 -1.0 361,890 + Psyr_0339 0.67 -0.7 361,891 - Psyr_0339 0.67 +0.8 361,891 - Psyr_0339 0.67 -1.5 361,891 - Psyr_0339 0.67 -0.9 361,891 - Psyr_0339 0.67 +0.6 361,891 - Psyr_0339 0.67 -0.5 361,891 - Psyr_0339 0.67 +0.2 361,891 - Psyr_0339 0.67 -0.3 361,891 - Psyr_0339 0.67 +0.1 362,052 + Psyr_0339 0.84 -1.3 362,052 + Psyr_0339 0.84 +0.7 362,052 + Psyr_0339 0.84 +0.9 362,052 + Psyr_0339 0.84 +0.7 362,052 + Psyr_0339 0.84 -1.9 362,053 - Psyr_0339 0.84 +1.3 362,053 - Psyr_0339 0.84 +0.2 362,053 - Psyr_0339 0.84 +0.0 362,053 - Psyr_0339 0.84 -0.6 362,079 + Psyr_0339 0.87 -0.5 362,079 + Psyr_0339 0.87 +0.3 362,079 + Psyr_0339 0.87 -0.1 362,079 + Psyr_0339 0.87 -0.8 362,079 + Psyr_0339 0.87 -0.1 362,079 + Psyr_0339 0.87 +0.9 362,079 + Psyr_0339 0.87 +0.6 362,079 + Psyr_0339 0.87 -0.2 362,080 - Psyr_0339 0.87 +0.6 362,080 - Psyr_0339 0.87 -0.3 362,080 - Psyr_0339 0.87 +0.1 362,080 - Psyr_0339 0.87 -2.5 362,080 - Psyr_0339 0.87 +0.0 362,080 - Psyr_0339 0.87 +0.2 362,080 - Psyr_0339 0.87 -0.5 362,218 + +1.2 362,606 + Psyr_0340 0.36 +1.0 362,606 + Psyr_0340 0.36 -0.8 362,606 + Psyr_0340 0.36 -1.2 362,607 - Psyr_0340 0.36 +0.0 362,607 - Psyr_0340 0.36 +1.6 362,607 - Psyr_0340 0.36 +2.4 362,607 - Psyr_0340 0.36 -0.1 362,607 - Psyr_0340 0.36 -0.3 362,747 + Psyr_0340 0.50 +0.4 362,747 + Psyr_0340 0.50 +1.1 362,747 + Psyr_0340 0.50 -0.4 362,747 + Psyr_0340 0.50 +1.7 362,748 - Psyr_0340 0.50 +0.1 362,748 - Psyr_0340 0.50 +2.1 362,748 - Psyr_0340 0.50 +0.6 362,748 - Psyr_0340 0.50 -0.3 362,748 - Psyr_0340 0.50 -0.1 362,887 + Psyr_0340 0.64 -0.1 362,887 + Psyr_0340 0.64 -1.5 362,887 + Psyr_0340 0.64 -1.6 362,887 + Psyr_0340 0.64 +0.0 362,887 + Psyr_0340 0.64 +0.9 362,887 + Psyr_0340 0.64 -0.8 362,888 - Psyr_0340 0.64 +0.2 362,888 - Psyr_0340 0.64 +0.8 362,888 - Psyr_0340 0.64 -0.3 362,903 + Psyr_0340 0.65 +0.0 362,903 + Psyr_0340 0.65 -0.4 362,903 + Psyr_0340 0.65 -0.3 362,904 - Psyr_0340 0.65 +0.0 362,904 - Psyr_0340 0.65 +0.0 362,942 + Psyr_0340 0.69 -0.9 362,942 + Psyr_0340 0.69 -0.2 362,942 + Psyr_0340 0.69 +0.2 362,943 - Psyr_0340 0.69 -1.4 362,943 - Psyr_0340 0.69 +0.2 362,943 - Psyr_0340 0.69 -0.4 362,943 - Psyr_0340 0.69 +2.1 362,943 - Psyr_0340 0.69 -0.3 362,943 - Psyr_0340 0.69 +2.6 362,981 + Psyr_0340 0.73 +2.5 362,981 + Psyr_0340 0.73 -0.9 362,981 + Psyr_0340 0.73 +0.8 362,982 - Psyr_0340 0.73 +0.7 362,982 - Psyr_0340 0.73 +1.1 362,993 + Psyr_0340 0.74 +0.2 362,993 + Psyr_0340 0.74 +0.0 362,993 + Psyr_0340 0.74 +0.8 362,994 - Psyr_0340 0.74 +0.1 362,994 - Psyr_0340 0.74 +0.8 363,056 + Psyr_0340 0.80 -0.2 363,056 + Psyr_0340 0.80 -1.8 363,056 + Psyr_0340 0.80 +0.4 363,056 + Psyr_0340 0.80 +1.2 363,056 + Psyr_0340 0.80 +1.0 363,056 + Psyr_0340 0.80 -1.4 363,056 + Psyr_0340 0.80 -0.6 363,057 - Psyr_0340 0.80 +0.3 363,057 - Psyr_0340 0.80 +0.5 363,057 - Psyr_0340 0.80 +0.8 363,057 - Psyr_0340 0.80 +0.7 363,057 - Psyr_0340 0.80 +0.8 363,159 - +0.1 363,159 - +1.9 363,323 + -2.0 363,323 + -0.2 363,323 + -1.2 363,323 + -1.4 363,324 - -0.3 363,324 - -0.7 363,348 - +0.6 363,401 + +0.1 363,401 + -0.9 363,401 + +0.2 363,401 + +0.1 363,402 - -0.7 363,452 + -0.3 363,453 - -1.2 363,453 - -2.0 363,453 - +0.6 363,453 - -0.5 363,453 - +0.5 363,453 - +1.1 363,453 - +0.1 363,544 - +1.4 363,606 + -0.7 363,606 + -0.2 363,607 - +0.2 363,658 + -1.2 363,658 + -2.1 363,658 + +0.2 363,658 + +0.7 363,659 - -0.0 363,659 - +1.0 363,659 - +1.7 363,659 - +0.4 363,659 - +0.3 363,839 - +0.7 363,869 - -0.4 363,869 - +0.1 363,876 + +0.3 363,877 - +0.8 363,952 + Psyr_0341 0.30 +0.6 363,952 + Psyr_0341 0.30 +0.3 363,952 + Psyr_0341 0.30 -0.3 363,952 + Psyr_0341 0.30 -0.9 363,953 - Psyr_0341 0.30 +0.4 363,953 - Psyr_0341 0.30 -0.6 363,953 - Psyr_0341 0.30 -1.6 363,953 - Psyr_0341 0.30 -0.7 363,953 - Psyr_0341 0.30 +0.8 363,979 - Psyr_0341 0.41 -0.5 364,113 + -0.2 364,113 + -0.3 364,122 + -0.2 364,122 + -1.3 364,122 + -0.4 364,122 + +0.0
Or see this region's nucleotide sequence