Experiment: Sucrose (C) and Glycine betaine (N); with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS02300 and PFLU_RS02305 overlap by 1 nucleotides PFLU_RS02305 and PFLU_RS02310 overlap by 4 nucleotides PFLU_RS02310 and PFLU_RS02315 overlap by 1 nucleotides
PFLU_RS02300: PFLU_RS02300 - lipopolysaccharide core heptose(I) kinase RfaP, at 526,461 to 527,267
_RS02300
PFLU_RS02305: PFLU_RS02305 - LPS biosynthesis protein, at 527,267 to 528,001
_RS02305
PFLU_RS02310: PFLU_RS02310 - LPS kinase, at 527,998 to 528,750
_RS02310
PFLU_RS02315: PFLU_RS02315 - serine/threonine protein kinase, at 528,750 to 530,201
_RS02315
Position (kb) 527
528
529 Strain fitness (log2 ratio) -1
0
1
2 at 528.160 kb on + strand, within PFLU_RS02310 at 528.163 kb on + strand, within PFLU_RS02310 at 528.163 kb on + strand, within PFLU_RS02310 at 528.163 kb on + strand, within PFLU_RS02310 at 528.184 kb on + strand, within PFLU_RS02310 at 528.246 kb on + strand, within PFLU_RS02310 at 528.295 kb on + strand, within PFLU_RS02310 at 528.295 kb on + strand, within PFLU_RS02310 at 528.295 kb on + strand, within PFLU_RS02310 at 528.295 kb on + strand, within PFLU_RS02310 at 528.498 kb on + strand, within PFLU_RS02310 at 528.498 kb on + strand, within PFLU_RS02310 at 528.498 kb on + strand, within PFLU_RS02310 at 528.498 kb on + strand, within PFLU_RS02310 at 528.498 kb on + strand, within PFLU_RS02310 at 528.498 kb on + strand, within PFLU_RS02310 at 528.526 kb on + strand, within PFLU_RS02310 at 528.526 kb on + strand, within PFLU_RS02310 at 528.526 kb on + strand, within PFLU_RS02310 at 528.729 kb on + strand at 528.729 kb on + strand at 528.729 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sucrose (C) and Glycine betaine (N); with MOPS remove 528,160 + PFLU_RS02310 0.22 +0.3 528,163 + PFLU_RS02310 0.22 +1.9 528,163 + PFLU_RS02310 0.22 +0.6 528,163 + PFLU_RS02310 0.22 -1.5 528,184 + PFLU_RS02310 0.25 -0.0 528,246 + PFLU_RS02310 0.33 +0.5 528,295 + PFLU_RS02310 0.39 -0.1 528,295 + PFLU_RS02310 0.39 +0.3 528,295 + PFLU_RS02310 0.39 +0.3 528,295 + PFLU_RS02310 0.39 +1.0 528,498 + PFLU_RS02310 0.66 -0.1 528,498 + PFLU_RS02310 0.66 +1.2 528,498 + PFLU_RS02310 0.66 -0.6 528,498 + PFLU_RS02310 0.66 +0.5 528,498 + PFLU_RS02310 0.66 -0.0 528,498 + PFLU_RS02310 0.66 -1.6 528,526 + PFLU_RS02310 0.70 +0.3 528,526 + PFLU_RS02310 0.70 -0.1 528,526 + PFLU_RS02310 0.70 +1.0 528,729 + -0.7 528,729 + +1.3 528,729 + +0.3
Or see this region's nucleotide sequence