Experiment: KB with Huperzine A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3550 and Psyr_3551 are separated by 429 nucleotides Psyr_3551 and Psyr_3552 are separated by 186 nucleotides Psyr_3552 and Psyr_3553 are separated by 325 nucleotides Psyr_3553 and Psyr_RNA54 are separated by 44 nucleotides
Psyr_3550: Psyr_3550 - conserved hypothetical protein, at 4,229,485 to 4,229,670
_3550
Psyr_3551: Psyr_3551 - Arc-like DNA binding protein, at 4,230,100 to 4,230,426
_3551
Psyr_3552: Psyr_3552 - Divalent cation transporter, at 4,230,613 to 4,232,055
_3552
Psyr_3553: Psyr_3553 - conserved hypothetical protein, at 4,232,381 to 4,232,872
_3553
Psyr_RNA54: Psyr_RNA54 - tRNA-Arg, at 4,232,917 to 4,232,993
_RNA54
Position (kb) 4230
4231
4232
4233 Strain fitness (log2 ratio) -2
-1
0
1 at 4229.680 kb on + strand at 4229.681 kb on - strand at 4229.681 kb on - strand at 4229.806 kb on + strand at 4229.833 kb on + strand at 4229.976 kb on + strand at 4229.977 kb on - strand at 4229.977 kb on - strand at 4229.977 kb on - strand at 4229.977 kb on - strand at 4229.977 kb on - strand at 4230.097 kb on + strand at 4230.098 kb on - strand at 4230.098 kb on - strand at 4230.099 kb on + strand at 4230.100 kb on - strand at 4230.100 kb on - strand at 4230.100 kb on - strand at 4230.100 kb on - strand at 4230.120 kb on + strand at 4230.121 kb on - strand at 4230.124 kb on + strand at 4230.124 kb on + strand at 4230.124 kb on + strand at 4230.125 kb on - strand at 4230.125 kb on - strand at 4230.125 kb on - strand at 4230.437 kb on - strand at 4230.475 kb on + strand at 4230.476 kb on - strand at 4230.486 kb on + strand at 4230.486 kb on + strand at 4230.487 kb on - strand at 4230.514 kb on + strand at 4230.514 kb on + strand at 4230.514 kb on + strand at 4230.515 kb on - strand at 4230.515 kb on - strand at 4230.523 kb on - strand at 4230.523 kb on - strand at 4230.599 kb on - strand at 4231.572 kb on - strand, within Psyr_3552 at 4231.572 kb on - strand, within Psyr_3552 at 4231.845 kb on - strand, within Psyr_3552 at 4231.845 kb on - strand, within Psyr_3552 at 4231.845 kb on - strand, within Psyr_3552 at 4231.845 kb on - strand, within Psyr_3552 at 4232.105 kb on - strand at 4232.105 kb on - strand at 4232.105 kb on - strand at 4232.135 kb on + strand at 4232.147 kb on - strand at 4232.198 kb on + strand at 4232.198 kb on + strand at 4232.198 kb on + strand at 4232.198 kb on + strand at 4232.198 kb on + strand at 4232.311 kb on + strand at 4232.312 kb on - strand at 4232.400 kb on + strand at 4232.400 kb on + strand at 4232.400 kb on + strand at 4232.400 kb on + strand at 4232.400 kb on + strand at 4232.400 kb on + strand at 4232.400 kb on + strand at 4232.400 kb on + strand at 4232.400 kb on + strand at 4232.401 kb on - strand at 4232.401 kb on - strand at 4232.401 kb on - strand at 4232.401 kb on - strand at 4232.485 kb on + strand, within Psyr_3553 at 4232.486 kb on - strand, within Psyr_3553 at 4232.486 kb on - strand, within Psyr_3553 at 4232.486 kb on - strand, within Psyr_3553 at 4232.492 kb on - strand, within Psyr_3553 at 4232.596 kb on + strand, within Psyr_3553 at 4232.596 kb on + strand, within Psyr_3553 at 4232.796 kb on - strand, within Psyr_3553 at 4232.801 kb on + strand, within Psyr_3553 at 4232.801 kb on + strand, within Psyr_3553 at 4232.802 kb on - strand, within Psyr_3553 at 4232.802 kb on - strand, within Psyr_3553 at 4232.802 kb on - strand, within Psyr_3553 at 4232.802 kb on - strand, within Psyr_3553 at 4232.802 kb on - strand, within Psyr_3553 at 4232.916 kb on - strand at 4232.916 kb on - strand at 4232.916 kb on - strand at 4232.916 kb on - strand at 4232.967 kb on + strand, within Psyr_RNA54 at 4232.986 kb on - strand at 4233.050 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Huperzine A 10 mM remove 4,229,680 + +1.0 4,229,681 - -0.1 4,229,681 - -1.7 4,229,806 + -0.0 4,229,833 + -0.7 4,229,976 + +0.5 4,229,977 - -0.9 4,229,977 - -1.1 4,229,977 - -0.7 4,229,977 - -0.3 4,229,977 - -1.7 4,230,097 + +1.4 4,230,098 - -0.6 4,230,098 - -0.9 4,230,099 + -0.9 4,230,100 - -1.8 4,230,100 - -1.8 4,230,100 - -0.7 4,230,100 - -1.4 4,230,120 + -0.0 4,230,121 - +0.7 4,230,124 + -1.3 4,230,124 + +0.1 4,230,124 + -0.3 4,230,125 - -0.0 4,230,125 - -0.9 4,230,125 - -1.4 4,230,437 - -1.3 4,230,475 + +0.7 4,230,476 - -1.4 4,230,486 + +0.2 4,230,486 + +0.3 4,230,487 - +0.4 4,230,514 + -0.4 4,230,514 + +1.3 4,230,514 + +0.3 4,230,515 - +1.5 4,230,515 - -1.7 4,230,523 - +0.3 4,230,523 - +0.4 4,230,599 - +0.0 4,231,572 - Psyr_3552 0.66 +0.3 4,231,572 - Psyr_3552 0.66 +0.3 4,231,845 - Psyr_3552 0.85 -1.8 4,231,845 - Psyr_3552 0.85 -1.1 4,231,845 - Psyr_3552 0.85 -0.3 4,231,845 - Psyr_3552 0.85 -0.6 4,232,105 - -0.6 4,232,105 - +1.6 4,232,105 - +1.7 4,232,135 + -0.9 4,232,147 - +0.3 4,232,198 + -1.3 4,232,198 + -0.1 4,232,198 + -1.4 4,232,198 + -0.6 4,232,198 + +1.6 4,232,311 + -0.5 4,232,312 - +0.8 4,232,400 + -0.6 4,232,400 + +0.1 4,232,400 + -1.5 4,232,400 + -0.2 4,232,400 + -1.4 4,232,400 + +0.2 4,232,400 + +0.8 4,232,400 + -0.0 4,232,400 + -2.1 4,232,401 - -1.2 4,232,401 - -1.3 4,232,401 - -0.7 4,232,401 - +1.6 4,232,485 + Psyr_3553 0.21 +0.2 4,232,486 - Psyr_3553 0.21 +1.7 4,232,486 - Psyr_3553 0.21 -0.3 4,232,486 - Psyr_3553 0.21 -1.1 4,232,492 - Psyr_3553 0.23 +1.7 4,232,596 + Psyr_3553 0.44 +0.1 4,232,596 + Psyr_3553 0.44 -0.9 4,232,796 - Psyr_3553 0.84 -1.2 4,232,801 + Psyr_3553 0.85 -0.1 4,232,801 + Psyr_3553 0.85 +0.3 4,232,802 - Psyr_3553 0.86 -0.5 4,232,802 - Psyr_3553 0.86 -0.3 4,232,802 - Psyr_3553 0.86 +0.1 4,232,802 - Psyr_3553 0.86 +0.4 4,232,802 - Psyr_3553 0.86 +0.4 4,232,916 - -0.3 4,232,916 - +0.3 4,232,916 - -1.4 4,232,916 - -0.4 4,232,967 + Psyr_RNA54 0.65 +1.3 4,232,986 - +0.3 4,233,050 - -0.1
Or see this region's nucleotide sequence