Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_0680

Experiment: KB with Huperzine A 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_RNA7 and Psyr_RNA8 are separated by 144 nucleotidesPsyr_RNA8 and Psyr_0680 are separated by 195 nucleotidesPsyr_0680 and Psyr_0681 overlap by 4 nucleotides Psyr_RNA7: Psyr_RNA7 - 23S ribosomal RNA, at 774,183 to 777,085 _RNA7 Psyr_RNA8: Psyr_RNA8 - 5S ribosomal RNA, at 777,230 to 777,344 _RNA8 Psyr_0680: Psyr_0680 - DNA helicase/exodeoxyribonuclease V, gamma subunit, at 777,540 to 781,013 _0680 Psyr_0681: Psyr_0681 - DNA helicase/exodeoxyribonuclease V, beta subunit, at 781,010 to 784,702 _0681 Position (kb) 777 778 779 780 781 782Strain fitness (log2 ratio) -1 0 1at 777.432 kb on + strandat 778.070 kb on - strand, within Psyr_0680at 778.398 kb on + strand, within Psyr_0680at 778.398 kb on + strand, within Psyr_0680at 778.398 kb on + strand, within Psyr_0680at 778.506 kb on + strand, within Psyr_0680at 778.847 kb on - strand, within Psyr_0680at 779.409 kb on + strand, within Psyr_0680at 779.772 kb on + strand, within Psyr_0680at 779.897 kb on + strand, within Psyr_0680at 780.156 kb on + strand, within Psyr_0680at 780.156 kb on + strand, within Psyr_0680at 781.743 kb on - strand, within Psyr_0681

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Huperzine A 10 mM
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777,432 + -1.3
778,070 - Psyr_0680 0.15 -0.9
778,398 + Psyr_0680 0.25 -1.4
778,398 + Psyr_0680 0.25 -1.3
778,398 + Psyr_0680 0.25 +0.2
778,506 + Psyr_0680 0.28 +0.4
778,847 - Psyr_0680 0.38 -0.8
779,409 + Psyr_0680 0.54 +0.1
779,772 + Psyr_0680 0.64 -0.1
779,897 + Psyr_0680 0.68 -1.1
780,156 + Psyr_0680 0.75 -0.0
780,156 + Psyr_0680 0.75 -1.1
781,743 - Psyr_0681 0.20 -0.9

Or see this region's nucleotide sequence