Experiment: KB with Khayasin C 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2723 and Psyr_2724 are separated by 131 nucleotides Psyr_2724 and Psyr_2725 are separated by 18 nucleotides Psyr_2725 and Psyr_2726 overlap by 4 nucleotides
Psyr_2723: Psyr_2723 - transcriptional regulator, LysR family, at 3,305,185 to 3,306,102
_2723
Psyr_2724: Psyr_2724 - conserved hypothetical protein, at 3,306,234 to 3,306,689
_2724
Psyr_2725: Psyr_2725 - Thiolase, at 3,306,708 to 3,307,970
_2725
Psyr_2726: Psyr_2726 - trans-feruloyl-CoA synthase, at 3,307,967 to 3,309,868
_2726
Position (kb)
3306
3307
3308 Strain fitness (log2 ratio)
-2
-1
0
1 at 3305.761 kb on + strand, within Psyr_2723 at 3305.761 kb on + strand, within Psyr_2723 at 3305.761 kb on + strand, within Psyr_2723 at 3305.762 kb on - strand, within Psyr_2723 at 3305.762 kb on - strand, within Psyr_2723 at 3305.762 kb on - strand, within Psyr_2723 at 3305.762 kb on - strand, within Psyr_2723 at 3305.762 kb on - strand, within Psyr_2723 at 3305.763 kb on + strand, within Psyr_2723 at 3306.053 kb on - strand at 3306.117 kb on - strand at 3306.155 kb on + strand at 3306.155 kb on + strand at 3306.155 kb on + strand at 3306.575 kb on + strand, within Psyr_2724 at 3306.575 kb on + strand, within Psyr_2724 at 3306.709 kb on + strand at 3306.727 kb on + strand at 3306.727 kb on + strand at 3306.728 kb on - strand at 3306.728 kb on - strand at 3306.728 kb on - strand at 3306.793 kb on + strand at 3306.843 kb on + strand, within Psyr_2725 at 3306.843 kb on + strand, within Psyr_2725 at 3306.843 kb on + strand, within Psyr_2725 at 3306.844 kb on - strand, within Psyr_2725 at 3306.931 kb on - strand, within Psyr_2725 at 3307.175 kb on + strand, within Psyr_2725 at 3307.175 kb on + strand, within Psyr_2725 at 3307.175 kb on + strand, within Psyr_2725 at 3307.176 kb on - strand, within Psyr_2725 at 3307.298 kb on + strand, within Psyr_2725 at 3307.299 kb on - strand, within Psyr_2725 at 3307.299 kb on - strand, within Psyr_2725 at 3307.499 kb on - strand, within Psyr_2725 at 3307.565 kb on + strand, within Psyr_2725 at 3307.567 kb on + strand, within Psyr_2725 at 3307.568 kb on - strand, within Psyr_2725 at 3307.682 kb on + strand, within Psyr_2725 at 3307.682 kb on + strand, within Psyr_2725 at 3307.683 kb on - strand, within Psyr_2725 at 3307.683 kb on - strand, within Psyr_2725 at 3307.727 kb on - strand, within Psyr_2725 at 3307.753 kb on + strand, within Psyr_2725 at 3307.754 kb on - strand, within Psyr_2725 at 3307.792 kb on - strand, within Psyr_2725 at 3307.952 kb on - strand at 3308.013 kb on + strand at 3308.014 kb on - strand at 3308.216 kb on + strand, within Psyr_2726 at 3308.338 kb on - strand, within Psyr_2726 at 3308.338 kb on - strand, within Psyr_2726 at 3308.493 kb on + strand, within Psyr_2726 at 3308.493 kb on + strand, within Psyr_2726 at 3308.493 kb on + strand, within Psyr_2726 at 3308.494 kb on - strand, within Psyr_2726 at 3308.494 kb on - strand, within Psyr_2726 at 3308.812 kb on + strand, within Psyr_2726 at 3308.895 kb on + strand, within Psyr_2726
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Khayasin C 10 mM remove 3,305,761 + Psyr_2723 0.63 -1.1 3,305,761 + Psyr_2723 0.63 +0.6 3,305,761 + Psyr_2723 0.63 -0.2 3,305,762 - Psyr_2723 0.63 -0.5 3,305,762 - Psyr_2723 0.63 -0.3 3,305,762 - Psyr_2723 0.63 +0.9 3,305,762 - Psyr_2723 0.63 -0.4 3,305,762 - Psyr_2723 0.63 +0.8 3,305,763 + Psyr_2723 0.63 +1.2 3,306,053 - +1.5 3,306,117 - -1.0 3,306,155 + -1.1 3,306,155 + -1.4 3,306,155 + -0.5 3,306,575 + Psyr_2724 0.75 +0.9 3,306,575 + Psyr_2724 0.75 -0.5 3,306,709 + +0.1 3,306,727 + +0.7 3,306,727 + -0.4 3,306,728 - -0.3 3,306,728 - -1.1 3,306,728 - -1.1 3,306,793 + +0.7 3,306,843 + Psyr_2725 0.11 +1.4 3,306,843 + Psyr_2725 0.11 -1.4 3,306,843 + Psyr_2725 0.11 -1.0 3,306,844 - Psyr_2725 0.11 +0.8 3,306,931 - Psyr_2725 0.18 -0.4 3,307,175 + Psyr_2725 0.37 -0.4 3,307,175 + Psyr_2725 0.37 +0.3 3,307,175 + Psyr_2725 0.37 -0.8 3,307,176 - Psyr_2725 0.37 -1.1 3,307,298 + Psyr_2725 0.47 +0.9 3,307,299 - Psyr_2725 0.47 +1.3 3,307,299 - Psyr_2725 0.47 -0.3 3,307,499 - Psyr_2725 0.63 +1.5 3,307,565 + Psyr_2725 0.68 -0.9 3,307,567 + Psyr_2725 0.68 +1.0 3,307,568 - Psyr_2725 0.68 +0.2 3,307,682 + Psyr_2725 0.77 +0.2 3,307,682 + Psyr_2725 0.77 -0.8 3,307,683 - Psyr_2725 0.77 +0.5 3,307,683 - Psyr_2725 0.77 +0.8 3,307,727 - Psyr_2725 0.81 -2.1 3,307,753 + Psyr_2725 0.83 +0.1 3,307,754 - Psyr_2725 0.83 -0.1 3,307,792 - Psyr_2725 0.86 +1.6 3,307,952 - -1.5 3,308,013 + -0.7 3,308,014 - -0.4 3,308,216 + Psyr_2726 0.13 -0.3 3,308,338 - Psyr_2726 0.20 +1.4 3,308,338 - Psyr_2726 0.20 +0.4 3,308,493 + Psyr_2726 0.28 +0.4 3,308,493 + Psyr_2726 0.28 -1.4 3,308,493 + Psyr_2726 0.28 +0.8 3,308,494 - Psyr_2726 0.28 +0.3 3,308,494 - Psyr_2726 0.28 +1.3 3,308,812 + Psyr_2726 0.44 +0.3 3,308,895 + Psyr_2726 0.49 +0.1
Or see this region's nucleotide sequence