Experiment: Phaeo_ML1_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c10090 and PGA1_c10100 are separated by 284 nucleotides PGA1_c10100 and PGA1_c10110 overlap by 8 nucleotides
PGA1_c10090: PGA1_c10090 - YeeE/YedE family (DUF395)., at 1,041,517 to 1,042,590
_c10090
PGA1_c10100: PGA1_c10100 - putative glutathione transport system permease protein GsiD, at 1,042,875 to 1,043,846
_c10100
PGA1_c10110: PGA1_c10110 - glutathione transport system permease protein GsiC, at 1,043,839 to 1,044,846
_c10110
Position (kb)
1042
1043
1044 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1041.880 kb on - strand, within PGA1_c10090 at 1041.880 kb on - strand, within PGA1_c10090 at 1042.119 kb on - strand, within PGA1_c10090 at 1042.172 kb on + strand, within PGA1_c10090 at 1042.172 kb on + strand, within PGA1_c10090 at 1042.243 kb on + strand, within PGA1_c10090 at 1042.388 kb on - strand, within PGA1_c10090 at 1042.436 kb on + strand, within PGA1_c10090 at 1042.436 kb on + strand, within PGA1_c10090 at 1042.801 kb on - strand at 1042.840 kb on - strand at 1043.283 kb on + strand, within PGA1_c10100 at 1043.309 kb on + strand, within PGA1_c10100 at 1043.427 kb on - strand, within PGA1_c10100 at 1043.483 kb on + strand, within PGA1_c10100 at 1043.581 kb on - strand, within PGA1_c10100 at 1043.737 kb on - strand, within PGA1_c10100 at 1043.779 kb on + strand at 1043.797 kb on - strand at 1043.832 kb on + strand at 1043.971 kb on + strand, within PGA1_c10110 at 1044.009 kb on + strand, within PGA1_c10110 at 1044.046 kb on + strand, within PGA1_c10110 at 1044.171 kb on + strand, within PGA1_c10110 at 1044.199 kb on - strand, within PGA1_c10110 at 1044.201 kb on - strand, within PGA1_c10110 at 1044.267 kb on + strand, within PGA1_c10110 at 1044.275 kb on - strand, within PGA1_c10110 at 1044.314 kb on + strand, within PGA1_c10110 at 1044.353 kb on - strand, within PGA1_c10110 at 1044.390 kb on + strand, within PGA1_c10110 at 1044.406 kb on - strand, within PGA1_c10110 at 1044.416 kb on - strand, within PGA1_c10110 at 1044.485 kb on - strand, within PGA1_c10110 at 1044.733 kb on + strand, within PGA1_c10110 at 1044.741 kb on - strand, within PGA1_c10110 at 1044.802 kb on + strand at 1044.811 kb on + strand at 1044.816 kb on + strand at 1044.824 kb on - strand at 1044.846 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phaeo_ML1_MIT diversity barseq remove 1,041,880 - PGA1_c10090 0.34 -0.4 1,041,880 - PGA1_c10090 0.34 -0.1 1,042,119 - PGA1_c10090 0.56 +1.0 1,042,172 + PGA1_c10090 0.61 +0.6 1,042,172 + PGA1_c10090 0.61 -1.2 1,042,243 + PGA1_c10090 0.68 -1.1 1,042,388 - PGA1_c10090 0.81 -0.1 1,042,436 + PGA1_c10090 0.86 +0.6 1,042,436 + PGA1_c10090 0.86 +2.1 1,042,801 - +0.6 1,042,840 - +0.6 1,043,283 + PGA1_c10100 0.42 -1.0 1,043,309 + PGA1_c10100 0.45 -0.1 1,043,427 - PGA1_c10100 0.57 -0.6 1,043,483 + PGA1_c10100 0.63 -1.0 1,043,581 - PGA1_c10100 0.73 +0.7 1,043,737 - PGA1_c10100 0.89 -0.6 1,043,779 + -0.4 1,043,797 - +0.1 1,043,832 + +1.3 1,043,971 + PGA1_c10110 0.13 +0.4 1,044,009 + PGA1_c10110 0.17 -0.8 1,044,046 + PGA1_c10110 0.21 +0.6 1,044,171 + PGA1_c10110 0.33 -0.4 1,044,199 - PGA1_c10110 0.36 +0.7 1,044,201 - PGA1_c10110 0.36 -1.9 1,044,267 + PGA1_c10110 0.42 +0.4 1,044,275 - PGA1_c10110 0.43 +0.1 1,044,314 + PGA1_c10110 0.47 -0.2 1,044,353 - PGA1_c10110 0.51 -0.6 1,044,390 + PGA1_c10110 0.55 +0.4 1,044,406 - PGA1_c10110 0.56 +2.0 1,044,416 - PGA1_c10110 0.57 -0.0 1,044,485 - PGA1_c10110 0.64 +1.8 1,044,733 + PGA1_c10110 0.89 -0.4 1,044,741 - PGA1_c10110 0.89 -1.1 1,044,802 + +0.9 1,044,811 + +1.8 1,044,816 + -0.1 1,044,824 - -0.2 1,044,846 - +0.7
Or see this region's nucleotide sequence