Experiment: Phaeo_ML1_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c24480 and PGA1_c24490 are separated by 57 nucleotides PGA1_c24490 and PGA1_c24500 are separated by 222 nucleotides PGA1_c24500 and PGA1_c24510 are separated by 296 nucleotides
PGA1_c24480: PGA1_c24480 - Predicted secreted protein, at 2,550,950 to 2,551,225
_c24480
PGA1_c24490: PGA1_c24490 - methylmalonyl-CoA epimerase, at 2,551,283 to 2,551,687
_c24490
PGA1_c24500: PGA1_c24500 - putative signal transduction response regulator receiver, at 2,551,910 to 2,552,629
_c24500
PGA1_c24510: PGA1_c24510 - Uncharacterized protein conserved in bacteria, at 2,552,926 to 2,554,149
_c24510
Position (kb)
2551
2552
2553 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2550.912 kb on - strand at 2550.934 kb on + strand at 2550.970 kb on + strand at 2550.978 kb on - strand, within PGA1_c24480 at 2551.281 kb on + strand at 2551.281 kb on + strand at 2551.696 kb on + strand at 2551.746 kb on - strand at 2551.748 kb on - strand at 2551.752 kb on + strand at 2551.835 kb on - strand at 2551.846 kb on + strand at 2551.884 kb on + strand at 2551.885 kb on - strand at 2551.909 kb on - strand at 2551.909 kb on - strand at 2552.018 kb on + strand, within PGA1_c24500 at 2552.024 kb on + strand, within PGA1_c24500 at 2552.109 kb on + strand, within PGA1_c24500 at 2552.164 kb on - strand, within PGA1_c24500 at 2552.195 kb on + strand, within PGA1_c24500 at 2552.228 kb on + strand, within PGA1_c24500 at 2552.320 kb on + strand, within PGA1_c24500 at 2552.369 kb on - strand, within PGA1_c24500 at 2552.415 kb on + strand, within PGA1_c24500 at 2552.415 kb on + strand, within PGA1_c24500 at 2552.431 kb on - strand, within PGA1_c24500 at 2552.433 kb on + strand, within PGA1_c24500 at 2552.441 kb on - strand, within PGA1_c24500 at 2552.450 kb on + strand, within PGA1_c24500 at 2552.455 kb on + strand, within PGA1_c24500 at 2552.468 kb on - strand, within PGA1_c24500 at 2552.478 kb on - strand, within PGA1_c24500 at 2552.501 kb on - strand, within PGA1_c24500 at 2552.593 kb on + strand at 2552.602 kb on - strand at 2552.656 kb on - strand at 2552.739 kb on + strand at 2552.763 kb on - strand at 2552.766 kb on - strand at 2552.806 kb on - strand at 2552.864 kb on + strand at 2552.872 kb on - strand at 2552.872 kb on - strand at 2552.890 kb on - strand at 2552.941 kb on + strand at 2552.949 kb on - strand at 2552.949 kb on - strand at 2552.958 kb on + strand at 2553.018 kb on + strand at 2553.047 kb on + strand at 2553.047 kb on + strand at 2553.047 kb on + strand at 2553.104 kb on - strand, within PGA1_c24510 at 2553.141 kb on - strand, within PGA1_c24510 at 2553.284 kb on + strand, within PGA1_c24510 at 2553.433 kb on + strand, within PGA1_c24510 at 2553.438 kb on + strand, within PGA1_c24510 at 2553.441 kb on - strand, within PGA1_c24510 at 2553.457 kb on + strand, within PGA1_c24510 at 2553.541 kb on - strand, within PGA1_c24510 at 2553.607 kb on + strand, within PGA1_c24510
Per-strain Table
Position Strand Gene LocusTag Fraction Phaeo_ML1_MIT diversity barseq remove 2,550,912 - -0.1 2,550,934 + +1.9 2,550,970 + +1.7 2,550,978 - PGA1_c24480 0.10 -0.3 2,551,281 + +0.7 2,551,281 + -0.4 2,551,696 + -0.1 2,551,746 - -0.9 2,551,748 - -0.2 2,551,752 + +1.6 2,551,835 - +0.4 2,551,846 + +1.2 2,551,884 + +0.9 2,551,885 - -0.3 2,551,909 - +0.5 2,551,909 - +0.2 2,552,018 + PGA1_c24500 0.15 -0.4 2,552,024 + PGA1_c24500 0.16 +0.4 2,552,109 + PGA1_c24500 0.28 -0.7 2,552,164 - PGA1_c24500 0.35 -0.9 2,552,195 + PGA1_c24500 0.40 -1.2 2,552,228 + PGA1_c24500 0.44 -1.6 2,552,320 + PGA1_c24500 0.57 -2.1 2,552,369 - PGA1_c24500 0.64 +0.0 2,552,415 + PGA1_c24500 0.70 -0.1 2,552,415 + PGA1_c24500 0.70 +0.2 2,552,431 - PGA1_c24500 0.72 +0.2 2,552,433 + PGA1_c24500 0.73 -2.1 2,552,441 - PGA1_c24500 0.74 -0.4 2,552,450 + PGA1_c24500 0.75 +1.7 2,552,455 + PGA1_c24500 0.76 -0.7 2,552,468 - PGA1_c24500 0.78 +0.4 2,552,478 - PGA1_c24500 0.79 +0.1 2,552,501 - PGA1_c24500 0.82 -2.2 2,552,593 + -1.2 2,552,602 - -0.2 2,552,656 - +0.1 2,552,739 + -0.5 2,552,763 - -0.4 2,552,766 - +0.9 2,552,806 - +0.8 2,552,864 + +2.9 2,552,872 - +0.4 2,552,872 - +0.6 2,552,890 - -0.5 2,552,941 + +0.2 2,552,949 - -0.9 2,552,949 - -0.1 2,552,958 + -0.5 2,553,018 + -1.4 2,553,047 + +0.6 2,553,047 + +0.3 2,553,047 + +0.4 2,553,104 - PGA1_c24510 0.15 +1.6 2,553,141 - PGA1_c24510 0.18 -0.2 2,553,284 + PGA1_c24510 0.29 +0.4 2,553,433 + PGA1_c24510 0.41 -1.2 2,553,438 + PGA1_c24510 0.42 -1.0 2,553,441 - PGA1_c24510 0.42 +1.6 2,553,457 + PGA1_c24510 0.43 -0.1 2,553,541 - PGA1_c24510 0.50 -0.2 2,553,607 + PGA1_c24510 0.56 +1.7
Or see this region's nucleotide sequence