Experiment: Phaeo_ML1_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c22360 and PGA1_c22370 are separated by 64 nucleotides PGA1_c22370 and PGA1_c22380 are separated by 399 nucleotides PGA1_c22380 and PGA1_c22390 are separated by 16 nucleotides PGA1_c22390 and PGA1_c22400 overlap by 4 nucleotides
PGA1_c22360: PGA1_c22360 - hypothetical protein, at 2,321,941 to 2,322,387
_c22360
PGA1_c22370: PGA1_c22370 - hypothetical protein, at 2,322,452 to 2,322,787
_c22370
PGA1_c22380: PGA1_c22380 - Bacteriophage protein of unknown function (DUF646)., at 2,323,187 to 2,323,642
_c22380
PGA1_c22390: PGA1_c22390 - Phage head-tail joining protein., at 2,323,659 to 2,324,015
_c22390
PGA1_c22400: PGA1_c22400 - phage conserved hypothetical protein, phiE125 gp8 family, at 2,324,012 to 2,324,617
_c22400
Position (kb)
2323
2324 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2322.204 kb on + strand, within PGA1_c22360 at 2322.244 kb on + strand, within PGA1_c22360 at 2322.252 kb on - strand, within PGA1_c22360 at 2322.272 kb on - strand, within PGA1_c22360 at 2322.292 kb on + strand, within PGA1_c22360 at 2322.363 kb on + strand at 2322.363 kb on + strand at 2322.363 kb on + strand at 2322.363 kb on + strand at 2322.363 kb on + strand at 2322.363 kb on + strand at 2322.363 kb on + strand at 2322.363 kb on + strand at 2322.363 kb on + strand at 2322.363 kb on + strand at 2322.363 kb on + strand at 2322.363 kb on + strand at 2322.363 kb on + strand at 2322.363 kb on + strand at 2322.363 kb on + strand at 2322.363 kb on + strand at 2322.363 kb on + strand at 2322.363 kb on + strand at 2322.365 kb on - strand at 2322.371 kb on - strand at 2322.371 kb on - strand at 2322.371 kb on - strand at 2322.371 kb on - strand at 2322.371 kb on - strand at 2322.371 kb on - strand at 2322.371 kb on - strand at 2322.371 kb on - strand at 2322.371 kb on - strand at 2322.371 kb on - strand at 2322.371 kb on - strand at 2322.371 kb on - strand at 2322.371 kb on - strand at 2322.390 kb on + strand at 2322.407 kb on - strand at 2322.431 kb on + strand at 2322.437 kb on - strand at 2322.459 kb on + strand at 2322.464 kb on + strand at 2322.503 kb on + strand, within PGA1_c22370 at 2322.597 kb on + strand, within PGA1_c22370 at 2322.597 kb on + strand, within PGA1_c22370 at 2322.597 kb on - strand, within PGA1_c22370 at 2322.597 kb on - strand, within PGA1_c22370 at 2322.605 kb on - strand, within PGA1_c22370 at 2322.605 kb on - strand, within PGA1_c22370 at 2322.624 kb on - strand, within PGA1_c22370 at 2322.643 kb on + strand, within PGA1_c22370 at 2322.643 kb on + strand, within PGA1_c22370 at 2322.682 kb on + strand, within PGA1_c22370 at 2322.682 kb on - strand, within PGA1_c22370 at 2322.748 kb on + strand, within PGA1_c22370 at 2322.756 kb on - strand at 2322.758 kb on - strand at 2322.790 kb on + strand at 2322.797 kb on + strand at 2322.806 kb on - strand at 2322.815 kb on + strand at 2322.815 kb on + strand at 2322.815 kb on - strand at 2322.841 kb on + strand at 2322.841 kb on + strand at 2322.858 kb on + strand at 2322.859 kb on - strand at 2322.876 kb on - strand at 2322.932 kb on + strand at 2322.936 kb on - strand at 2322.942 kb on + strand at 2322.950 kb on - strand at 2322.995 kb on + strand at 2323.028 kb on - strand at 2323.107 kb on + strand at 2323.109 kb on - strand at 2323.115 kb on - strand at 2323.146 kb on + strand at 2323.146 kb on + strand at 2323.146 kb on + strand at 2323.146 kb on + strand at 2323.146 kb on + strand at 2323.151 kb on + strand at 2323.154 kb on - strand at 2323.154 kb on - strand at 2323.154 kb on - strand at 2323.156 kb on + strand at 2323.174 kb on + strand at 2323.219 kb on - strand at 2323.289 kb on - strand, within PGA1_c22380 at 2323.289 kb on - strand, within PGA1_c22380 at 2323.289 kb on - strand, within PGA1_c22380 at 2323.298 kb on + strand, within PGA1_c22380 at 2323.298 kb on - strand, within PGA1_c22380 at 2323.372 kb on + strand, within PGA1_c22380 at 2323.380 kb on - strand, within PGA1_c22380 at 2323.380 kb on - strand, within PGA1_c22380 at 2323.403 kb on + strand, within PGA1_c22380 at 2323.404 kb on + strand, within PGA1_c22380 at 2323.445 kb on - strand, within PGA1_c22380 at 2323.471 kb on + strand, within PGA1_c22380 at 2323.471 kb on + strand, within PGA1_c22380 at 2323.492 kb on + strand, within PGA1_c22380 at 2323.527 kb on + strand, within PGA1_c22380 at 2323.535 kb on - strand, within PGA1_c22380 at 2323.567 kb on + strand, within PGA1_c22380 at 2323.588 kb on - strand, within PGA1_c22380 at 2323.650 kb on + strand at 2323.672 kb on + strand at 2323.718 kb on - strand, within PGA1_c22390 at 2323.728 kb on + strand, within PGA1_c22390 at 2323.773 kb on + strand, within PGA1_c22390 at 2323.782 kb on + strand, within PGA1_c22390 at 2323.815 kb on + strand, within PGA1_c22390 at 2323.828 kb on + strand, within PGA1_c22390 at 2323.828 kb on + strand, within PGA1_c22390 at 2323.874 kb on - strand, within PGA1_c22390 at 2323.892 kb on + strand, within PGA1_c22390 at 2323.916 kb on + strand, within PGA1_c22390 at 2323.924 kb on - strand, within PGA1_c22390 at 2323.926 kb on + strand, within PGA1_c22390 at 2323.992 kb on - strand at 2323.993 kb on - strand at 2323.993 kb on - strand at 2324.003 kb on - strand at 2324.010 kb on + strand at 2324.086 kb on - strand, within PGA1_c22400 at 2324.147 kb on - strand, within PGA1_c22400 at 2324.168 kb on - strand, within PGA1_c22400 at 2324.202 kb on + strand, within PGA1_c22400 at 2324.271 kb on + strand, within PGA1_c22400 at 2324.271 kb on + strand, within PGA1_c22400 at 2324.271 kb on + strand, within PGA1_c22400 at 2324.271 kb on + strand, within PGA1_c22400 at 2324.279 kb on - strand, within PGA1_c22400 at 2324.279 kb on - strand, within PGA1_c22400 at 2324.394 kb on - strand, within PGA1_c22400 at 2324.408 kb on - strand, within PGA1_c22400 at 2324.452 kb on - strand, within PGA1_c22400 at 2324.515 kb on + strand, within PGA1_c22400 at 2324.523 kb on - strand, within PGA1_c22400 at 2324.574 kb on - strand at 2324.596 kb on + strand at 2324.596 kb on + strand at 2324.604 kb on + strand at 2324.604 kb on - strand at 2324.638 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phaeo_ML1_MIT diversity barseq remove 2,322,204 + PGA1_c22360 0.59 +0.1 2,322,244 + PGA1_c22360 0.68 +0.9 2,322,252 - PGA1_c22360 0.70 -1.2 2,322,272 - PGA1_c22360 0.74 +0.9 2,322,292 + PGA1_c22360 0.79 -0.3 2,322,363 + -0.3 2,322,363 + +0.6 2,322,363 + +1.3 2,322,363 + +0.2 2,322,363 + -0.2 2,322,363 + -0.9 2,322,363 + -1.5 2,322,363 + +0.6 2,322,363 + -1.7 2,322,363 + +0.8 2,322,363 + -0.2 2,322,363 + +0.1 2,322,363 + +3.4 2,322,363 + -0.6 2,322,363 + +1.0 2,322,363 + -1.4 2,322,363 + +0.3 2,322,363 + +0.7 2,322,365 - +0.8 2,322,371 - +0.2 2,322,371 - +0.1 2,322,371 - -0.9 2,322,371 - -0.7 2,322,371 - -0.0 2,322,371 - +1.0 2,322,371 - -0.2 2,322,371 - -0.1 2,322,371 - +0.0 2,322,371 - -0.0 2,322,371 - -0.2 2,322,371 - -0.6 2,322,371 - +0.1 2,322,390 + +0.1 2,322,407 - -0.3 2,322,431 + +1.7 2,322,437 - -2.2 2,322,459 + -0.2 2,322,464 + -2.1 2,322,503 + PGA1_c22370 0.15 +0.2 2,322,597 + PGA1_c22370 0.43 +0.1 2,322,597 + PGA1_c22370 0.43 +0.1 2,322,597 - PGA1_c22370 0.43 -0.8 2,322,597 - PGA1_c22370 0.43 -0.1 2,322,605 - PGA1_c22370 0.46 +0.9 2,322,605 - PGA1_c22370 0.46 +0.4 2,322,624 - PGA1_c22370 0.51 -0.2 2,322,643 + PGA1_c22370 0.57 -0.6 2,322,643 + PGA1_c22370 0.57 -0.6 2,322,682 + PGA1_c22370 0.68 -0.1 2,322,682 - PGA1_c22370 0.68 +0.6 2,322,748 + PGA1_c22370 0.88 +1.9 2,322,756 - +0.2 2,322,758 - -0.4 2,322,790 + -0.3 2,322,797 + +0.5 2,322,806 - -1.1 2,322,815 + -0.3 2,322,815 + +2.1 2,322,815 - +1.3 2,322,841 + -0.5 2,322,841 + +1.6 2,322,858 + +0.4 2,322,859 - -0.6 2,322,876 - -0.4 2,322,932 + -0.0 2,322,936 - +0.8 2,322,942 + -0.0 2,322,950 - +1.4 2,322,995 + -0.4 2,323,028 - +0.0 2,323,107 + -1.2 2,323,109 - -2.2 2,323,115 - +0.6 2,323,146 + +0.6 2,323,146 + +0.3 2,323,146 + -1.6 2,323,146 + -0.4 2,323,146 + +0.6 2,323,151 + +0.9 2,323,154 - -0.3 2,323,154 - -0.4 2,323,154 - -0.3 2,323,156 + -0.2 2,323,174 + +0.3 2,323,219 - +2.0 2,323,289 - PGA1_c22380 0.22 +0.1 2,323,289 - PGA1_c22380 0.22 -1.6 2,323,289 - PGA1_c22380 0.22 +0.1 2,323,298 + PGA1_c22380 0.24 +0.4 2,323,298 - PGA1_c22380 0.24 +0.1 2,323,372 + PGA1_c22380 0.41 -2.0 2,323,380 - PGA1_c22380 0.42 -0.1 2,323,380 - PGA1_c22380 0.42 +0.3 2,323,403 + PGA1_c22380 0.47 +0.3 2,323,404 + PGA1_c22380 0.48 -0.2 2,323,445 - PGA1_c22380 0.57 -0.3 2,323,471 + PGA1_c22380 0.62 -0.9 2,323,471 + PGA1_c22380 0.62 -1.3 2,323,492 + PGA1_c22380 0.67 +0.8 2,323,527 + PGA1_c22380 0.75 -1.9 2,323,535 - PGA1_c22380 0.76 -1.0 2,323,567 + PGA1_c22380 0.83 -0.6 2,323,588 - PGA1_c22380 0.88 -0.6 2,323,650 + +2.4 2,323,672 + +0.3 2,323,718 - PGA1_c22390 0.17 -1.1 2,323,728 + PGA1_c22390 0.19 +0.6 2,323,773 + PGA1_c22390 0.32 +0.1 2,323,782 + PGA1_c22390 0.34 -0.1 2,323,815 + PGA1_c22390 0.44 +1.1 2,323,828 + PGA1_c22390 0.47 -0.3 2,323,828 + PGA1_c22390 0.47 +2.0 2,323,874 - PGA1_c22390 0.60 +1.1 2,323,892 + PGA1_c22390 0.65 +0.0 2,323,916 + PGA1_c22390 0.72 -0.9 2,323,924 - PGA1_c22390 0.74 -0.1 2,323,926 + PGA1_c22390 0.75 +0.9 2,323,992 - +0.2 2,323,993 - -0.5 2,323,993 - +2.0 2,324,003 - +0.0 2,324,010 + -1.0 2,324,086 - PGA1_c22400 0.12 -0.4 2,324,147 - PGA1_c22400 0.22 +1.0 2,324,168 - PGA1_c22400 0.26 -1.2 2,324,202 + PGA1_c22400 0.31 +1.5 2,324,271 + PGA1_c22400 0.43 -0.4 2,324,271 + PGA1_c22400 0.43 +0.5 2,324,271 + PGA1_c22400 0.43 -0.4 2,324,271 + PGA1_c22400 0.43 +0.4 2,324,279 - PGA1_c22400 0.44 -0.3 2,324,279 - PGA1_c22400 0.44 -0.5 2,324,394 - PGA1_c22400 0.63 +0.1 2,324,408 - PGA1_c22400 0.65 +0.3 2,324,452 - PGA1_c22400 0.73 +0.2 2,324,515 + PGA1_c22400 0.83 -0.9 2,324,523 - PGA1_c22400 0.84 -0.4 2,324,574 - +0.0 2,324,596 + -0.6 2,324,596 + +0.6 2,324,604 + -0.4 2,324,604 - +0.1 2,324,638 - +0.6
Or see this region's nucleotide sequence