Experiment: Phaeo_ML1_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c11050 and PGA1_c11060 are separated by 215 nucleotides PGA1_c11060 and PGA1_c11070 are separated by 206 nucleotides
PGA1_c11050: PGA1_c11050 - FAD dependent oxidoreductase, at 1,143,458 to 1,144,684
_c11050
PGA1_c11060: PGA1_c11060 - cold shock-like protein CspE, at 1,144,900 to 1,145,106
_c11060
PGA1_c11070: PGA1_c11070 - exodeoxyribonuclease III, at 1,145,313 to 1,146,119
_c11070
Position (kb)
1144
1145
1146 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1144.634 kb on - strand at 1144.678 kb on + strand at 1144.678 kb on + strand at 1144.678 kb on + strand at 1144.678 kb on + strand at 1144.678 kb on + strand at 1144.678 kb on + strand at 1144.678 kb on + strand at 1144.678 kb on + strand at 1144.678 kb on + strand at 1144.686 kb on - strand at 1144.686 kb on - strand at 1144.765 kb on + strand at 1144.765 kb on + strand at 1144.819 kb on + strand at 1144.827 kb on - strand at 1144.839 kb on + strand at 1144.892 kb on + strand at 1144.963 kb on + strand, within PGA1_c11060 at 1144.970 kb on + strand, within PGA1_c11060 at 1145.011 kb on + strand, within PGA1_c11060 at 1145.011 kb on + strand, within PGA1_c11060 at 1145.011 kb on + strand, within PGA1_c11060 at 1145.011 kb on + strand, within PGA1_c11060 at 1145.011 kb on + strand, within PGA1_c11060 at 1145.011 kb on + strand, within PGA1_c11060 at 1145.011 kb on + strand, within PGA1_c11060 at 1145.011 kb on + strand, within PGA1_c11060 at 1145.011 kb on + strand, within PGA1_c11060 at 1145.011 kb on + strand, within PGA1_c11060 at 1145.011 kb on + strand, within PGA1_c11060 at 1145.011 kb on + strand, within PGA1_c11060 at 1145.011 kb on + strand, within PGA1_c11060 at 1145.019 kb on - strand, within PGA1_c11060 at 1145.019 kb on - strand, within PGA1_c11060 at 1145.019 kb on - strand, within PGA1_c11060 at 1145.019 kb on - strand, within PGA1_c11060 at 1145.019 kb on - strand, within PGA1_c11060 at 1145.019 kb on - strand, within PGA1_c11060 at 1145.019 kb on - strand, within PGA1_c11060 at 1145.136 kb on + strand at 1145.203 kb on - strand at 1145.219 kb on + strand at 1145.219 kb on + strand at 1145.227 kb on - strand at 1145.227 kb on - strand at 1145.227 kb on - strand at 1145.332 kb on - strand at 1145.503 kb on - strand, within PGA1_c11070 at 1145.620 kb on + strand, within PGA1_c11070 at 1145.716 kb on - strand, within PGA1_c11070 at 1145.721 kb on - strand, within PGA1_c11070 at 1145.858 kb on + strand, within PGA1_c11070 at 1145.930 kb on + strand, within PGA1_c11070 at 1145.930 kb on + strand, within PGA1_c11070 at 1145.952 kb on + strand, within PGA1_c11070 at 1145.960 kb on - strand, within PGA1_c11070 at 1146.010 kb on + strand, within PGA1_c11070 at 1146.035 kb on + strand, within PGA1_c11070
Per-strain Table
Position Strand Gene LocusTag Fraction Phaeo_ML1_MIT diversity barseq remove 1,144,634 - -1.8 1,144,678 + +0.6 1,144,678 + +1.1 1,144,678 + +0.3 1,144,678 + -0.4 1,144,678 + +0.9 1,144,678 + +0.6 1,144,678 + -0.5 1,144,678 + -0.0 1,144,678 + +2.1 1,144,686 - +1.2 1,144,686 - +0.1 1,144,765 + -1.0 1,144,765 + -0.0 1,144,819 + -0.0 1,144,827 - +0.1 1,144,839 + -0.9 1,144,892 + -0.0 1,144,963 + PGA1_c11060 0.30 -0.2 1,144,970 + PGA1_c11060 0.34 -1.0 1,145,011 + PGA1_c11060 0.54 +0.7 1,145,011 + PGA1_c11060 0.54 -1.0 1,145,011 + PGA1_c11060 0.54 -0.8 1,145,011 + PGA1_c11060 0.54 +0.1 1,145,011 + PGA1_c11060 0.54 -0.3 1,145,011 + PGA1_c11060 0.54 -0.0 1,145,011 + PGA1_c11060 0.54 -0.3 1,145,011 + PGA1_c11060 0.54 -0.3 1,145,011 + PGA1_c11060 0.54 -2.7 1,145,011 + PGA1_c11060 0.54 -0.8 1,145,011 + PGA1_c11060 0.54 -0.2 1,145,011 + PGA1_c11060 0.54 +0.1 1,145,011 + PGA1_c11060 0.54 +0.4 1,145,019 - PGA1_c11060 0.57 -0.1 1,145,019 - PGA1_c11060 0.57 -1.4 1,145,019 - PGA1_c11060 0.57 -1.0 1,145,019 - PGA1_c11060 0.57 +1.1 1,145,019 - PGA1_c11060 0.57 -1.1 1,145,019 - PGA1_c11060 0.57 -0.9 1,145,019 - PGA1_c11060 0.57 -2.4 1,145,136 + -1.6 1,145,203 - -0.5 1,145,219 + -1.0 1,145,219 + +1.6 1,145,227 - -0.6 1,145,227 - -0.0 1,145,227 - +1.1 1,145,332 - +1.0 1,145,503 - PGA1_c11070 0.24 +0.1 1,145,620 + PGA1_c11070 0.38 -0.0 1,145,716 - PGA1_c11070 0.50 +0.4 1,145,721 - PGA1_c11070 0.51 -0.4 1,145,858 + PGA1_c11070 0.68 -1.0 1,145,930 + PGA1_c11070 0.76 -1.8 1,145,930 + PGA1_c11070 0.76 +0.1 1,145,952 + PGA1_c11070 0.79 -2.8 1,145,960 - PGA1_c11070 0.80 +1.4 1,146,010 + PGA1_c11070 0.86 -0.3 1,146,035 + PGA1_c11070 0.89 -2.6
Or see this region's nucleotide sequence