Experiment: nitrogen source Ammonium chloride 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_567 and Pf1N1B4_568 are separated by 133 nucleotides Pf1N1B4_568 and Pf1N1B4_569 overlap by 4 nucleotides Pf1N1B4_569 and Pf1N1B4_570 are separated by 172 nucleotides Pf1N1B4_570 and Pf1N1B4_571 are separated by 191 nucleotides
Pf1N1B4_567 - Histone acetyltransferase HPA2 and related acetyltransferases, at 662,801 to 663,220
_567
Pf1N1B4_568 - Peptide deformylase (EC 3.5.1.88), at 663,354 to 663,893
_568
Pf1N1B4_569 - Ribonuclease BN (EC 3.1.-.-), at 663,890 to 664,834
_569
Pf1N1B4_570 - hypothetical protein, at 665,007 to 665,198
_570
Pf1N1B4_571 - FIG00954271: hypothetical protein, at 665,390 to 665,767
_571
Position (kb) 663
664
665 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 662.943 kb on + strand at 662.943 kb on + strand, within Pf1N1B4_567 at 662.943 kb on + strand, within Pf1N1B4_567 at 662.943 kb on + strand, within Pf1N1B4_567 at 663.102 kb on - strand at 663.102 kb on - strand, within Pf1N1B4_567 at 663.102 kb on - strand, within Pf1N1B4_567 at 663.118 kb on + strand, within Pf1N1B4_567 at 663.119 kb on - strand, within Pf1N1B4_567 at 663.277 kb on - strand at 663.439 kb on - strand, within Pf1N1B4_568 at 664.060 kb on + strand, within Pf1N1B4_569 at 664.061 kb on - strand at 664.061 kb on - strand, within Pf1N1B4_569 at 664.062 kb on + strand, within Pf1N1B4_569 at 664.063 kb on - strand, within Pf1N1B4_569 at 664.063 kb on - strand, within Pf1N1B4_569 at 664.063 kb on - strand, within Pf1N1B4_569 at 664.063 kb on - strand at 664.063 kb on - strand, within Pf1N1B4_569 at 664.069 kb on - strand, within Pf1N1B4_569 at 664.104 kb on + strand, within Pf1N1B4_569 at 664.104 kb on + strand at 664.105 kb on - strand, within Pf1N1B4_569 at 664.105 kb on - strand, within Pf1N1B4_569 at 664.117 kb on - strand, within Pf1N1B4_569 at 664.117 kb on - strand at 664.137 kb on + strand, within Pf1N1B4_569 at 664.137 kb on + strand at 664.138 kb on - strand, within Pf1N1B4_569 at 664.202 kb on - strand, within Pf1N1B4_569 at 664.268 kb on + strand, within Pf1N1B4_569 at 664.416 kb on + strand at 664.470 kb on + strand, within Pf1N1B4_569 at 664.471 kb on - strand, within Pf1N1B4_569 at 664.471 kb on - strand, within Pf1N1B4_569 at 664.471 kb on - strand, within Pf1N1B4_569 at 664.746 kb on + strand at 664.746 kb on + strand at 664.747 kb on - strand at 664.747 kb on - strand at 664.747 kb on - strand at 664.747 kb on - strand at 664.747 kb on - strand at 664.747 kb on - strand at 664.747 kb on - strand at 664.747 kb on - strand at 664.783 kb on + strand at 664.784 kb on - strand at 664.892 kb on + strand at 664.892 kb on + strand at 664.907 kb on + strand at 664.907 kb on + strand at 664.907 kb on + strand at 664.907 kb on + strand at 664.907 kb on + strand at 664.936 kb on + strand at 664.936 kb on + strand at 664.936 kb on + strand at 664.938 kb on + strand at 664.938 kb on + strand at 664.938 kb on + strand at 664.938 kb on + strand at 664.938 kb on + strand at 664.938 kb on + strand at 664.938 kb on + strand at 665.009 kb on - strand at 665.180 kb on + strand at 665.201 kb on + strand at 665.201 kb on + strand at 665.202 kb on - strand at 665.278 kb on + strand at 665.282 kb on + strand at 665.282 kb on + strand at 665.283 kb on - strand at 665.305 kb on + strand at 665.312 kb on + strand at 665.313 kb on - strand at 665.347 kb on + strand at 665.347 kb on + strand at 665.347 kb on + strand at 665.347 kb on + strand at 665.347 kb on + strand at 665.348 kb on - strand at 665.497 kb on + strand, within Pf1N1B4_571 at 665.498 kb on - strand, within Pf1N1B4_571 at 665.498 kb on - strand, within Pf1N1B4_571 at 665.599 kb on + strand, within Pf1N1B4_571 at 665.599 kb on + strand, within Pf1N1B4_571 at 665.599 kb on + strand, within Pf1N1B4_571 at 665.599 kb on + strand, within Pf1N1B4_571 at 665.599 kb on + strand, within Pf1N1B4_571 at 665.599 kb on + strand, within Pf1N1B4_571 at 665.599 kb on + strand, within Pf1N1B4_571 at 665.599 kb on + strand, within Pf1N1B4_571 at 665.600 kb on - strand at 665.600 kb on - strand, within Pf1N1B4_571 at 665.600 kb on - strand, within Pf1N1B4_571 at 665.600 kb on - strand, within Pf1N1B4_571 at 665.708 kb on + strand, within Pf1N1B4_571 at 665.709 kb on - strand, within Pf1N1B4_571 at 665.709 kb on - strand, within Pf1N1B4_571
Per-strain Table
Position Strand Gene LocusTag Fraction nitrogen source Ammonium chloride 20mM remove 662,943 + -1.8 662,943 + Pf1N1B4_567 0.34 -0.8 662,943 + Pf1N1B4_567 0.34 -1.6 662,943 + Pf1N1B4_567 0.34 -0.9 663,102 - -2.6 663,102 - Pf1N1B4_567 0.72 -0.6 663,102 - Pf1N1B4_567 0.72 -0.2 663,118 + Pf1N1B4_567 0.75 -0.2 663,119 - Pf1N1B4_567 0.76 -0.3 663,277 - -0.3 663,439 - Pf1N1B4_568 0.16 +1.5 664,060 + Pf1N1B4_569 0.18 -0.4 664,061 - +0.4 664,061 - Pf1N1B4_569 0.18 +0.5 664,062 + Pf1N1B4_569 0.18 +0.6 664,063 - Pf1N1B4_569 0.18 +1.1 664,063 - Pf1N1B4_569 0.18 +0.8 664,063 - Pf1N1B4_569 0.18 +1.0 664,063 - +0.6 664,063 - Pf1N1B4_569 0.18 -1.0 664,069 - Pf1N1B4_569 0.19 +0.8 664,104 + Pf1N1B4_569 0.23 -0.0 664,104 + +3.3 664,105 - Pf1N1B4_569 0.23 +1.5 664,105 - Pf1N1B4_569 0.23 +0.7 664,117 - Pf1N1B4_569 0.24 +1.4 664,117 - -0.7 664,137 + Pf1N1B4_569 0.26 +1.2 664,137 + -0.0 664,138 - Pf1N1B4_569 0.26 +0.3 664,202 - Pf1N1B4_569 0.33 +0.7 664,268 + Pf1N1B4_569 0.40 +0.2 664,416 + +2.4 664,470 + Pf1N1B4_569 0.61 +0.4 664,471 - Pf1N1B4_569 0.61 -0.8 664,471 - Pf1N1B4_569 0.61 +0.6 664,471 - Pf1N1B4_569 0.61 -0.0 664,746 + +1.1 664,746 + -0.1 664,747 - -0.4 664,747 - +0.2 664,747 - +0.4 664,747 - +0.2 664,747 - +1.8 664,747 - +1.4 664,747 - -1.2 664,747 - +1.6 664,783 + +0.1 664,784 - -1.0 664,892 + +0.3 664,892 + +0.4 664,907 + +0.2 664,907 + -0.4 664,907 + -1.3 664,907 + +1.1 664,907 + +1.8 664,936 + -1.2 664,936 + +1.1 664,936 + -0.2 664,938 + -0.0 664,938 + -1.3 664,938 + -0.8 664,938 + -0.3 664,938 + +1.2 664,938 + -0.7 664,938 + -0.3 665,009 - +0.7 665,180 + -1.7 665,201 + -0.3 665,201 + -0.6 665,202 - -0.7 665,278 + -0.0 665,282 + -1.6 665,282 + +1.4 665,283 - -1.5 665,305 + +2.0 665,312 + +0.4 665,313 - -0.3 665,347 + +0.8 665,347 + -0.9 665,347 + +0.4 665,347 + -0.8 665,347 + +1.0 665,348 - -1.6 665,497 + Pf1N1B4_571 0.28 -1.0 665,498 - Pf1N1B4_571 0.29 +1.3 665,498 - Pf1N1B4_571 0.29 -0.5 665,599 + Pf1N1B4_571 0.55 -0.6 665,599 + Pf1N1B4_571 0.55 +0.5 665,599 + Pf1N1B4_571 0.55 -0.1 665,599 + Pf1N1B4_571 0.55 +0.1 665,599 + Pf1N1B4_571 0.55 +1.3 665,599 + Pf1N1B4_571 0.55 -1.3 665,599 + Pf1N1B4_571 0.55 -1.3 665,599 + Pf1N1B4_571 0.55 +0.4 665,600 - +0.2 665,600 - Pf1N1B4_571 0.56 -0.0 665,600 - Pf1N1B4_571 0.56 -0.1 665,600 - Pf1N1B4_571 0.56 +0.1 665,708 + Pf1N1B4_571 0.84 -1.2 665,709 - Pf1N1B4_571 0.84 -0.2 665,709 - Pf1N1B4_571 0.84 +0.6
Or see this region's nucleotide sequence