Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_07095 and AO353_07100 are separated by 7 nucleotides AO353_07100 and AO353_07105 overlap by 11 nucleotides AO353_07105 and AO353_07110 are separated by 140 nucleotides AO353_07110 and AO353_07115 are separated by 140 nucleotides
AO353_07095: AO353_07095 - hypothetical protein, at 1,559,813 to 1,560,259
_07095
AO353_07100: AO353_07100 - type III pantothenate kinase, at 1,560,267 to 1,561,016
_07100
AO353_07105: AO353_07105 - biotin--protein ligase, at 1,561,006 to 1,561,965
_07105
AO353_07110: AO353_07110 - 5S ribosomal RNA, at 1,562,106 to 1,562,221
_07110
AO353_07115: AO353_07115 - 23S ribosomal RNA, at 1,562,362 to 1,565,277
_07115
Position (kb)
1561
1562 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1560.051 kb on + strand, within AO353_07095 at 1560.051 kb on + strand, within AO353_07095 at 1560.052 kb on - strand, within AO353_07095 at 1560.082 kb on + strand, within AO353_07095 at 1560.082 kb on + strand, within AO353_07095 at 1560.082 kb on + strand, within AO353_07095 at 1560.083 kb on - strand, within AO353_07095 at 1560.083 kb on - strand, within AO353_07095 at 1560.083 kb on - strand, within AO353_07095 at 1560.083 kb on - strand, within AO353_07095 at 1560.101 kb on + strand, within AO353_07095 at 1560.101 kb on + strand, within AO353_07095 at 1560.101 kb on + strand, within AO353_07095 at 1560.101 kb on + strand, within AO353_07095 at 1560.101 kb on + strand, within AO353_07095 at 1560.101 kb on + strand, within AO353_07095 at 1560.101 kb on + strand, within AO353_07095 at 1560.101 kb on + strand, within AO353_07095 at 1560.102 kb on - strand, within AO353_07095 at 1560.102 kb on - strand, within AO353_07095 at 1560.102 kb on - strand, within AO353_07095 at 1560.102 kb on - strand, within AO353_07095 at 1560.151 kb on + strand, within AO353_07095 at 1560.151 kb on + strand, within AO353_07095 at 1560.151 kb on + strand, within AO353_07095 at 1560.152 kb on - strand, within AO353_07095 at 1560.152 kb on - strand, within AO353_07095 at 1560.153 kb on + strand, within AO353_07095 at 1560.153 kb on + strand, within AO353_07095 at 1560.153 kb on + strand, within AO353_07095 at 1560.153 kb on + strand, within AO353_07095 at 1560.153 kb on + strand, within AO353_07095 at 1560.153 kb on + strand, within AO353_07095 at 1560.153 kb on + strand, within AO353_07095 at 1560.153 kb on + strand, within AO353_07095 at 1560.154 kb on - strand, within AO353_07095 at 1560.154 kb on - strand, within AO353_07095 at 1560.154 kb on - strand, within AO353_07095 at 1560.154 kb on - strand, within AO353_07095 at 1560.154 kb on - strand, within AO353_07095 at 1560.154 kb on - strand, within AO353_07095 at 1560.154 kb on - strand, within AO353_07095 at 1560.154 kb on - strand, within AO353_07095 at 1560.213 kb on + strand, within AO353_07095 at 1560.213 kb on + strand, within AO353_07095 at 1560.213 kb on + strand, within AO353_07095 at 1560.214 kb on - strand, within AO353_07095 at 1560.216 kb on + strand at 1560.216 kb on + strand at 1560.217 kb on - strand at 1560.217 kb on - strand at 1560.217 kb on - strand at 1560.217 kb on - strand at 1560.259 kb on + strand at 1560.259 kb on + strand at 1560.259 kb on + strand at 1560.259 kb on + strand at 1560.260 kb on - strand at 1560.260 kb on - strand at 1560.260 kb on - strand at 1560.260 kb on - strand at 1560.304 kb on - strand at 1561.034 kb on - strand at 1561.034 kb on - strand at 1561.034 kb on - strand at 1561.834 kb on - strand, within AO353_07105 at 1561.891 kb on - strand at 1561.894 kb on - strand at 1561.894 kb on - strand at 1561.894 kb on - strand at 1561.894 kb on - strand at 1561.894 kb on - strand at 1561.894 kb on - strand at 1561.898 kb on - strand at 1561.898 kb on - strand at 1561.898 kb on - strand at 1561.898 kb on - strand at 1561.898 kb on - strand at 1561.898 kb on - strand at 1561.952 kb on - strand at 1561.952 kb on - strand at 1561.952 kb on - strand at 1561.952 kb on - strand at 1561.952 kb on - strand at 1561.972 kb on - strand at 1561.998 kb on + strand at 1561.999 kb on - strand at 1561.999 kb on - strand at 1562.021 kb on + strand at 1562.021 kb on + strand at 1562.021 kb on + strand at 1562.021 kb on + strand at 1562.022 kb on - strand at 1562.022 kb on - strand at 1562.022 kb on - strand at 1562.022 kb on - strand at 1562.022 kb on - strand at 1562.030 kb on + strand at 1562.030 kb on + strand at 1562.030 kb on + strand at 1562.031 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 1,560,051 + AO353_07095 0.53 -0.7 1,560,051 + AO353_07095 0.53 -0.7 1,560,052 - AO353_07095 0.53 +2.8 1,560,082 + AO353_07095 0.60 +1.3 1,560,082 + AO353_07095 0.60 +0.8 1,560,082 + AO353_07095 0.60 +1.8 1,560,083 - AO353_07095 0.60 -0.2 1,560,083 - AO353_07095 0.60 -2.2 1,560,083 - AO353_07095 0.60 +0.8 1,560,083 - AO353_07095 0.60 +1.8 1,560,101 + AO353_07095 0.64 -0.5 1,560,101 + AO353_07095 0.64 +0.3 1,560,101 + AO353_07095 0.64 +0.3 1,560,101 + AO353_07095 0.64 +1.8 1,560,101 + AO353_07095 0.64 -1.7 1,560,101 + AO353_07095 0.64 -1.0 1,560,101 + AO353_07095 0.64 +0.8 1,560,101 + AO353_07095 0.64 -0.9 1,560,102 - AO353_07095 0.65 +0.0 1,560,102 - AO353_07095 0.65 -1.3 1,560,102 - AO353_07095 0.65 -0.9 1,560,102 - AO353_07095 0.65 -1.7 1,560,151 + AO353_07095 0.76 +1.8 1,560,151 + AO353_07095 0.76 -0.5 1,560,151 + AO353_07095 0.76 +0.3 1,560,152 - AO353_07095 0.76 +1.3 1,560,152 - AO353_07095 0.76 -0.3 1,560,153 + AO353_07095 0.76 -0.0 1,560,153 + AO353_07095 0.76 -0.2 1,560,153 + AO353_07095 0.76 -2.8 1,560,153 + AO353_07095 0.76 +0.5 1,560,153 + AO353_07095 0.76 +0.8 1,560,153 + AO353_07095 0.76 -0.5 1,560,153 + AO353_07095 0.76 +0.3 1,560,153 + AO353_07095 0.76 +1.3 1,560,154 - AO353_07095 0.76 +0.3 1,560,154 - AO353_07095 0.76 +2.3 1,560,154 - AO353_07095 0.76 +0.8 1,560,154 - AO353_07095 0.76 -1.7 1,560,154 - AO353_07095 0.76 -0.3 1,560,154 - AO353_07095 0.76 -1.3 1,560,154 - AO353_07095 0.76 -0.0 1,560,154 - AO353_07095 0.76 -1.7 1,560,213 + AO353_07095 0.89 +0.8 1,560,213 + AO353_07095 0.89 +1.1 1,560,213 + AO353_07095 0.89 -1.4 1,560,214 - AO353_07095 0.90 +0.3 1,560,216 + +0.3 1,560,216 + +0.3 1,560,217 - +1.2 1,560,217 - -1.6 1,560,217 - +2.4 1,560,217 - -1.2 1,560,259 + +0.0 1,560,259 + -1.3 1,560,259 + -0.7 1,560,259 + +1.4 1,560,260 - -0.7 1,560,260 - +0.8 1,560,260 - -0.9 1,560,260 - +1.8 1,560,304 - +0.3 1,561,034 - +0.3 1,561,034 - +1.8 1,561,034 - +0.3 1,561,834 - AO353_07105 0.86 -1.4 1,561,891 - -1.0 1,561,894 - +0.8 1,561,894 - -0.3 1,561,894 - -2.0 1,561,894 - -1.0 1,561,894 - -2.2 1,561,894 - -1.2 1,561,898 - -2.6 1,561,898 - -2.7 1,561,898 - -1.9 1,561,898 - -1.3 1,561,898 - -0.7 1,561,898 - -1.4 1,561,952 - +0.8 1,561,952 - -3.3 1,561,952 - +1.8 1,561,952 - +0.4 1,561,952 - +0.3 1,561,972 - -0.8 1,561,998 + -0.5 1,561,999 - -0.2 1,561,999 - -0.1 1,562,021 + +0.8 1,562,021 + +0.3 1,562,021 + -0.5 1,562,021 + +0.3 1,562,022 - -0.2 1,562,022 - +1.8 1,562,022 - -1.2 1,562,022 - +1.2 1,562,022 - +1.0 1,562,030 + -2.6 1,562,030 + -0.3 1,562,030 + -0.9 1,562,031 - -2.0
Or see this region's nucleotide sequence