Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_483

Experiment: RCH2 defined with Thallium(I) acetate 0.002 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_481 and Pf6N2E2_482 are separated by 192 nucleotidesPf6N2E2_482 and Pf6N2E2_483 are separated by 151 nucleotidesPf6N2E2_483 and Pf6N2E2_484 are separated by 320 nucleotides Pf6N2E2_481 - branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit (EC 1.2.4.4) (from data), at 547,221 to 548,456 _481 Pf6N2E2_482 - Transcriptional regulator BkdR of isoleucine and valine catabolism operon, at 548,649 to 549,137 _482 Pf6N2E2_483 - FIG146518: Zn-dependent hydrolases, including glyoxylases, at 549,289 to 550,152 _483 Pf6N2E2_484 - Aldose 1-epimerase, at 550,473 to 551,342 _484 Position (kb) 549 550 551Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 548.345 kb on + strandat 548.377 kb on + strandat 548.378 kb on - strandat 548.464 kb on + strandat 548.465 kb on - strandat 548.465 kb on - strandat 548.465 kb on - strandat 548.489 kb on + strandat 548.502 kb on - strandat 548.537 kb on + strandat 548.538 kb on - strandat 548.548 kb on + strandat 548.549 kb on - strandat 548.549 kb on - strandat 548.829 kb on - strand, within Pf6N2E2_482at 548.943 kb on + strand, within Pf6N2E2_482at 548.943 kb on + strand, within Pf6N2E2_482at 549.290 kb on + strandat 549.291 kb on - strandat 549.291 kb on - strandat 549.291 kb on - strandat 549.317 kb on + strandat 549.317 kb on + strandat 549.318 kb on - strandat 549.533 kb on + strand, within Pf6N2E2_483at 549.548 kb on + strand, within Pf6N2E2_483at 549.548 kb on + strand, within Pf6N2E2_483at 549.548 kb on + strand, within Pf6N2E2_483at 549.548 kb on + strand, within Pf6N2E2_483at 549.549 kb on - strand, within Pf6N2E2_483at 549.549 kb on - strand, within Pf6N2E2_483at 549.549 kb on - strand, within Pf6N2E2_483at 549.596 kb on + strand, within Pf6N2E2_483at 549.597 kb on - strand, within Pf6N2E2_483at 549.597 kb on - strand, within Pf6N2E2_483at 549.698 kb on + strand, within Pf6N2E2_483at 549.911 kb on + strand, within Pf6N2E2_483at 549.912 kb on - strandat 549.912 kb on - strand, within Pf6N2E2_483at 550.100 kb on + strandat 550.100 kb on + strandat 550.100 kb on + strandat 550.100 kb on + strandat 550.100 kb on + strandat 550.101 kb on - strandat 550.101 kb on - strandat 550.101 kb on - strandat 550.106 kb on + strandat 550.106 kb on + strandat 550.106 kb on + strandat 550.106 kb on + strandat 550.106 kb on + strandat 550.107 kb on - strandat 550.107 kb on - strandat 550.107 kb on - strandat 550.107 kb on - strandat 550.237 kb on + strandat 550.243 kb on - strandat 550.266 kb on + strandat 550.267 kb on - strandat 550.267 kb on - strandat 550.267 kb on - strandat 550.267 kb on - strandat 550.288 kb on - strandat 550.288 kb on - strandat 550.474 kb on + strandat 550.621 kb on + strand, within Pf6N2E2_484at 550.622 kb on - strand, within Pf6N2E2_484at 550.633 kb on + strand, within Pf6N2E2_484at 550.633 kb on + strand, within Pf6N2E2_484at 550.634 kb on - strand, within Pf6N2E2_484at 550.659 kb on + strand, within Pf6N2E2_484at 550.659 kb on + strand, within Pf6N2E2_484at 550.659 kb on + strand, within Pf6N2E2_484at 550.659 kb on + strand, within Pf6N2E2_484at 550.660 kb on - strand, within Pf6N2E2_484at 550.660 kb on - strand, within Pf6N2E2_484at 550.660 kb on - strand, within Pf6N2E2_484at 550.671 kb on + strand, within Pf6N2E2_484at 550.671 kb on + strand, within Pf6N2E2_484at 550.671 kb on + strand, within Pf6N2E2_484at 550.672 kb on - strand, within Pf6N2E2_484at 550.672 kb on - strand, within Pf6N2E2_484at 550.672 kb on - strand, within Pf6N2E2_484at 550.672 kb on - strand, within Pf6N2E2_484at 550.672 kb on - strand, within Pf6N2E2_484at 550.672 kb on - strand, within Pf6N2E2_484at 550.672 kb on - strand, within Pf6N2E2_484at 550.672 kb on - strand, within Pf6N2E2_484at 550.743 kb on + strand, within Pf6N2E2_484at 550.870 kb on + strand, within Pf6N2E2_484at 550.871 kb on - strand, within Pf6N2E2_484

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2 defined with Thallium(I) acetate 0.002 mg/ml
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548,345 + +1.9
548,377 + +0.5
548,378 - +1.1
548,464 + +2.4
548,465 - -1.5
548,465 - +0.8
548,465 - -2.2
548,489 + +0.5
548,502 - -0.6
548,537 + +1.4
548,538 - -0.8
548,548 + +0.5
548,549 - -0.0
548,549 - +0.5
548,829 - Pf6N2E2_482 0.37 -2.0
548,943 + Pf6N2E2_482 0.60 +1.0
548,943 + Pf6N2E2_482 0.60 +1.6
549,290 + -1.4
549,291 - -0.3
549,291 - +0.9
549,291 - -0.8
549,317 + -0.6
549,317 + -2.9
549,318 - +0.7
549,533 + Pf6N2E2_483 0.28 +1.0
549,548 + Pf6N2E2_483 0.30 -1.0
549,548 + Pf6N2E2_483 0.30 -0.4
549,548 + Pf6N2E2_483 0.30 -1.3
549,548 + Pf6N2E2_483 0.30 -0.2
549,549 - Pf6N2E2_483 0.30 -0.6
549,549 - Pf6N2E2_483 0.30 +0.1
549,549 - Pf6N2E2_483 0.30 +0.0
549,596 + Pf6N2E2_483 0.36 +0.1
549,597 - Pf6N2E2_483 0.36 -0.4
549,597 - Pf6N2E2_483 0.36 -0.8
549,698 + Pf6N2E2_483 0.47 -0.1
549,911 + Pf6N2E2_483 0.72 -1.6
549,912 - +0.6
549,912 - Pf6N2E2_483 0.72 -1.0
550,100 + -0.3
550,100 + -0.3
550,100 + +0.3
550,100 + +0.1
550,100 + +0.2
550,101 - -0.1
550,101 - +0.0
550,101 - -0.4
550,106 + -1.4
550,106 + +0.8
550,106 + +1.6
550,106 + -0.1
550,106 + +1.0
550,107 - +1.9
550,107 - -1.0
550,107 - +2.6
550,107 - +0.7
550,237 + -2.3
550,243 - -0.4
550,266 + -0.1
550,267 - -1.0
550,267 - -2.6
550,267 - -1.2
550,267 - +0.9
550,288 - -0.4
550,288 - +1.6
550,474 + -1.3
550,621 + Pf6N2E2_484 0.17 -0.5
550,622 - Pf6N2E2_484 0.17 -1.6
550,633 + Pf6N2E2_484 0.18 -0.5
550,633 + Pf6N2E2_484 0.18 +1.5
550,634 - Pf6N2E2_484 0.19 +0.0
550,659 + Pf6N2E2_484 0.21 +0.1
550,659 + Pf6N2E2_484 0.21 -1.0
550,659 + Pf6N2E2_484 0.21 +2.5
550,659 + Pf6N2E2_484 0.21 -0.6
550,660 - Pf6N2E2_484 0.21 +0.2
550,660 - Pf6N2E2_484 0.21 +0.5
550,660 - Pf6N2E2_484 0.21 +0.7
550,671 + Pf6N2E2_484 0.23 +0.3
550,671 + Pf6N2E2_484 0.23 +1.0
550,671 + Pf6N2E2_484 0.23 +1.7
550,672 - Pf6N2E2_484 0.23 -0.1
550,672 - Pf6N2E2_484 0.23 +0.5
550,672 - Pf6N2E2_484 0.23 +1.1
550,672 - Pf6N2E2_484 0.23 -1.0
550,672 - Pf6N2E2_484 0.23 -0.5
550,672 - Pf6N2E2_484 0.23 -2.0
550,672 - Pf6N2E2_484 0.23 -1.5
550,672 - Pf6N2E2_484 0.23 +1.0
550,743 + Pf6N2E2_484 0.31 +0.1
550,870 + Pf6N2E2_484 0.46 -0.5
550,871 - Pf6N2E2_484 0.46 +2.5

Or see this region's nucleotide sequence