Experiment: RCH2 defined with copper (II) chloride 0.03 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1655 and Pf6N2E2_1656 are separated by 155 nucleotides Pf6N2E2_1656 and Pf6N2E2_1657 are separated by 141 nucleotides Pf6N2E2_1657 and Pf6N2E2_1658 are separated by 78 nucleotides Pf6N2E2_1658 and Pf6N2E2_1659 are separated by 18 nucleotides Pf6N2E2_1659 and Pf6N2E2_1660 are separated by 113 nucleotides
Pf6N2E2_1655 - Bifunctional protein: zinc-containing alcohol dehydrogenase; quinone oxidoreductase ( NADPH:quinone reductase) (EC 1.1.1.-); Similar to arginate lyase, at 1,961,277 to 1,962,293
_1655
Pf6N2E2_1656 - hypothetical protein, at 1,962,449 to 1,962,619
_1656
Pf6N2E2_1657 - NAD glycohydrolase, hvnA; Halovibrin, at 1,962,761 to 1,963,996
_1657
Pf6N2E2_1658 - hypothetical protein, at 1,964,075 to 1,964,323
_1658
Pf6N2E2_1659 - Inositol transport system sugar-binding protein, at 1,964,342 to 1,964,455
_1659
Pf6N2E2_1660 - RND efflux system, membrane fusion protein CmeA, at 1,964,569 to 1,965,738
_1660
Position (kb)
1962
1963
1964 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1961.857 kb on - strand, within Pf6N2E2_1655 at 1961.893 kb on - strand, within Pf6N2E2_1655 at 1961.893 kb on - strand, within Pf6N2E2_1655 at 1961.893 kb on - strand, within Pf6N2E2_1655 at 1962.065 kb on - strand, within Pf6N2E2_1655 at 1962.065 kb on - strand, within Pf6N2E2_1655 at 1962.096 kb on + strand, within Pf6N2E2_1655 at 1962.097 kb on - strand, within Pf6N2E2_1655 at 1962.108 kb on + strand, within Pf6N2E2_1655 at 1962.108 kb on + strand, within Pf6N2E2_1655 at 1962.109 kb on - strand, within Pf6N2E2_1655 at 1962.109 kb on - strand, within Pf6N2E2_1655 at 1962.109 kb on - strand, within Pf6N2E2_1655 at 1962.225 kb on + strand at 1962.225 kb on + strand at 1962.225 kb on + strand at 1962.226 kb on - strand at 1962.226 kb on - strand at 1962.551 kb on + strand, within Pf6N2E2_1656 at 1962.551 kb on + strand, within Pf6N2E2_1656 at 1962.552 kb on - strand, within Pf6N2E2_1656 at 1962.876 kb on - strand at 1963.058 kb on + strand, within Pf6N2E2_1657 at 1963.091 kb on + strand, within Pf6N2E2_1657 at 1963.116 kb on + strand, within Pf6N2E2_1657 at 1963.117 kb on - strand, within Pf6N2E2_1657 at 1963.145 kb on + strand, within Pf6N2E2_1657 at 1963.145 kb on + strand, within Pf6N2E2_1657 at 1963.146 kb on - strand, within Pf6N2E2_1657 at 1963.541 kb on + strand, within Pf6N2E2_1657 at 1963.542 kb on - strand, within Pf6N2E2_1657 at 1963.664 kb on + strand, within Pf6N2E2_1657 at 1963.665 kb on - strand, within Pf6N2E2_1657 at 1963.678 kb on + strand, within Pf6N2E2_1657 at 1963.679 kb on - strand, within Pf6N2E2_1657 at 1963.679 kb on - strand, within Pf6N2E2_1657 at 1963.799 kb on + strand, within Pf6N2E2_1657 at 1963.799 kb on + strand, within Pf6N2E2_1657 at 1963.799 kb on + strand, within Pf6N2E2_1657 at 1963.835 kb on + strand, within Pf6N2E2_1657 at 1963.869 kb on + strand, within Pf6N2E2_1657 at 1963.869 kb on + strand, within Pf6N2E2_1657 at 1963.869 kb on + strand, within Pf6N2E2_1657 at 1963.869 kb on + strand, within Pf6N2E2_1657 at 1963.869 kb on + strand, within Pf6N2E2_1657 at 1963.869 kb on + strand, within Pf6N2E2_1657 at 1963.869 kb on + strand, within Pf6N2E2_1657 at 1963.870 kb on - strand, within Pf6N2E2_1657 at 1963.870 kb on - strand, within Pf6N2E2_1657 at 1963.870 kb on - strand, within Pf6N2E2_1657 at 1963.870 kb on - strand, within Pf6N2E2_1657 at 1963.870 kb on - strand, within Pf6N2E2_1657 at 1963.870 kb on - strand, within Pf6N2E2_1657 at 1963.870 kb on - strand, within Pf6N2E2_1657 at 1963.870 kb on - strand, within Pf6N2E2_1657 at 1963.870 kb on - strand, within Pf6N2E2_1657 at 1963.870 kb on - strand, within Pf6N2E2_1657 at 1963.949 kb on + strand at 1963.949 kb on + strand at 1963.950 kb on - strand at 1963.979 kb on + strand at 1963.980 kb on - strand at 1963.980 kb on - strand at 1964.001 kb on + strand at 1964.097 kb on - strand at 1964.302 kb on - strand at 1964.313 kb on - strand at 1964.313 kb on - strand at 1964.366 kb on - strand, within Pf6N2E2_1659 at 1964.481 kb on - strand at 1964.481 kb on - strand at 1964.502 kb on - strand at 1964.723 kb on + strand, within Pf6N2E2_1660 at 1964.723 kb on + strand, within Pf6N2E2_1660 at 1964.723 kb on + strand, within Pf6N2E2_1660
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with copper (II) chloride 0.03 mM remove 1,961,857 - Pf6N2E2_1655 0.57 -0.0 1,961,893 - Pf6N2E2_1655 0.61 -0.1 1,961,893 - Pf6N2E2_1655 0.61 +1.7 1,961,893 - Pf6N2E2_1655 0.61 -0.4 1,962,065 - Pf6N2E2_1655 0.77 +0.6 1,962,065 - Pf6N2E2_1655 0.77 +0.5 1,962,096 + Pf6N2E2_1655 0.81 +1.0 1,962,097 - Pf6N2E2_1655 0.81 -0.1 1,962,108 + Pf6N2E2_1655 0.82 +2.2 1,962,108 + Pf6N2E2_1655 0.82 +0.6 1,962,109 - Pf6N2E2_1655 0.82 +0.1 1,962,109 - Pf6N2E2_1655 0.82 -0.0 1,962,109 - Pf6N2E2_1655 0.82 +0.9 1,962,225 + +0.2 1,962,225 + -2.5 1,962,225 + -2.1 1,962,226 - +1.1 1,962,226 - -1.1 1,962,551 + Pf6N2E2_1656 0.60 +0.0 1,962,551 + Pf6N2E2_1656 0.60 -1.7 1,962,552 - Pf6N2E2_1656 0.60 -1.4 1,962,876 - +0.4 1,963,058 + Pf6N2E2_1657 0.24 +0.4 1,963,091 + Pf6N2E2_1657 0.27 -1.4 1,963,116 + Pf6N2E2_1657 0.29 -1.0 1,963,117 - Pf6N2E2_1657 0.29 -0.0 1,963,145 + Pf6N2E2_1657 0.31 -0.1 1,963,145 + Pf6N2E2_1657 0.31 -1.4 1,963,146 - Pf6N2E2_1657 0.31 +0.5 1,963,541 + Pf6N2E2_1657 0.63 +2.2 1,963,542 - Pf6N2E2_1657 0.63 -2.2 1,963,664 + Pf6N2E2_1657 0.73 +0.6 1,963,665 - Pf6N2E2_1657 0.73 -1.2 1,963,678 + Pf6N2E2_1657 0.74 +0.3 1,963,679 - Pf6N2E2_1657 0.74 +0.6 1,963,679 - Pf6N2E2_1657 0.74 -0.5 1,963,799 + Pf6N2E2_1657 0.84 -1.0 1,963,799 + Pf6N2E2_1657 0.84 -0.9 1,963,799 + Pf6N2E2_1657 0.84 +0.6 1,963,835 + Pf6N2E2_1657 0.87 +0.0 1,963,869 + Pf6N2E2_1657 0.90 -1.2 1,963,869 + Pf6N2E2_1657 0.90 +1.0 1,963,869 + Pf6N2E2_1657 0.90 +0.6 1,963,869 + Pf6N2E2_1657 0.90 -1.5 1,963,869 + Pf6N2E2_1657 0.90 +0.5 1,963,869 + Pf6N2E2_1657 0.90 -2.2 1,963,869 + Pf6N2E2_1657 0.90 -0.7 1,963,870 - Pf6N2E2_1657 0.90 -2.0 1,963,870 - Pf6N2E2_1657 0.90 +0.3 1,963,870 - Pf6N2E2_1657 0.90 -0.4 1,963,870 - Pf6N2E2_1657 0.90 +0.8 1,963,870 - Pf6N2E2_1657 0.90 -1.8 1,963,870 - Pf6N2E2_1657 0.90 +1.0 1,963,870 - Pf6N2E2_1657 0.90 +0.8 1,963,870 - Pf6N2E2_1657 0.90 -0.3 1,963,870 - Pf6N2E2_1657 0.90 -2.4 1,963,870 - Pf6N2E2_1657 0.90 -3.1 1,963,949 + -0.7 1,963,949 + -2.8 1,963,950 - -1.0 1,963,979 + -1.0 1,963,980 - +0.7 1,963,980 - -0.4 1,964,001 + +0.8 1,964,097 - +0.6 1,964,302 - +1.2 1,964,313 - +0.7 1,964,313 - +1.5 1,964,366 - Pf6N2E2_1659 0.21 +0.8 1,964,481 - -0.2 1,964,481 - +0.1 1,964,502 - +0.5 1,964,723 + Pf6N2E2_1660 0.13 -0.4 1,964,723 + Pf6N2E2_1660 0.13 -0.4 1,964,723 + Pf6N2E2_1660 0.13 +0.6
Or see this region's nucleotide sequence