Experiment: RCH2 defined with copper (II) chloride 0.03 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4383 and Pf6N2E2_4384 are separated by 183 nucleotides Pf6N2E2_4384 and Pf6N2E2_4385 are separated by 121 nucleotides
Pf6N2E2_4383 - gamma-glutamyl-gamma-aminobutyraldehyde dehydrogenase (EC 1.2.1.99); 4-guanidinobutyraldehyde dehydrogenase (EC 1.2.1.54) (from data) , at 5,110,461 to 5,111,954
_4383
Pf6N2E2_4384 - Major facilitator family transporter, at 5,112,138 to 5,113,280
_4384
Pf6N2E2_4385 - Cardiolipin synthetase (EC 2.7.8.-), at 5,113,402 to 5,114,967
_4385
Position (kb)
5112
5113
5114 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5111.162 kb on + strand, within Pf6N2E2_4383 at 5111.162 kb on + strand, within Pf6N2E2_4383 at 5111.163 kb on - strand, within Pf6N2E2_4383 at 5111.163 kb on - strand, within Pf6N2E2_4383 at 5111.163 kb on - strand, within Pf6N2E2_4383 at 5111.174 kb on + strand, within Pf6N2E2_4383 at 5111.223 kb on + strand, within Pf6N2E2_4383 at 5111.224 kb on - strand, within Pf6N2E2_4383 at 5111.224 kb on - strand, within Pf6N2E2_4383 at 5111.224 kb on - strand, within Pf6N2E2_4383 at 5111.224 kb on - strand, within Pf6N2E2_4383 at 5111.224 kb on - strand, within Pf6N2E2_4383 at 5111.249 kb on + strand, within Pf6N2E2_4383 at 5111.249 kb on + strand, within Pf6N2E2_4383 at 5111.249 kb on + strand, within Pf6N2E2_4383 at 5111.249 kb on + strand, within Pf6N2E2_4383 at 5111.250 kb on - strand, within Pf6N2E2_4383 at 5111.526 kb on + strand, within Pf6N2E2_4383 at 5111.526 kb on + strand, within Pf6N2E2_4383 at 5111.527 kb on - strand, within Pf6N2E2_4383 at 5111.600 kb on + strand, within Pf6N2E2_4383 at 5111.600 kb on + strand, within Pf6N2E2_4383 at 5111.601 kb on - strand, within Pf6N2E2_4383 at 5111.601 kb on - strand, within Pf6N2E2_4383 at 5111.604 kb on + strand, within Pf6N2E2_4383 at 5111.604 kb on + strand, within Pf6N2E2_4383 at 5111.604 kb on + strand, within Pf6N2E2_4383 at 5111.604 kb on + strand, within Pf6N2E2_4383 at 5111.604 kb on + strand, within Pf6N2E2_4383 at 5111.604 kb on + strand, within Pf6N2E2_4383 at 5111.604 kb on + strand, within Pf6N2E2_4383 at 5111.604 kb on + strand, within Pf6N2E2_4383 at 5111.605 kb on - strand, within Pf6N2E2_4383 at 5111.605 kb on - strand, within Pf6N2E2_4383 at 5111.605 kb on - strand, within Pf6N2E2_4383 at 5111.605 kb on - strand, within Pf6N2E2_4383 at 5111.605 kb on - strand, within Pf6N2E2_4383 at 5111.605 kb on - strand, within Pf6N2E2_4383 at 5111.771 kb on + strand, within Pf6N2E2_4383 at 5111.771 kb on + strand, within Pf6N2E2_4383 at 5111.772 kb on - strand, within Pf6N2E2_4383 at 5111.832 kb on + strand at 5111.832 kb on + strand at 5111.832 kb on + strand at 5111.920 kb on - strand at 5111.920 kb on - strand at 5111.920 kb on - strand at 5111.920 kb on - strand at 5111.920 kb on - strand at 5111.920 kb on - strand at 5111.920 kb on - strand at 5111.984 kb on + strand at 5112.028 kb on + strand at 5112.029 kb on - strand at 5112.115 kb on + strand at 5112.115 kb on + strand at 5112.115 kb on + strand at 5112.115 kb on + strand at 5112.115 kb on + strand at 5112.116 kb on - strand at 5112.116 kb on - strand at 5112.116 kb on - strand at 5112.116 kb on - strand at 5112.116 kb on - strand at 5112.116 kb on - strand at 5112.153 kb on + strand at 5112.153 kb on + strand at 5112.154 kb on - strand at 5112.154 kb on - strand at 5112.244 kb on - strand at 5112.364 kb on + strand, within Pf6N2E2_4384 at 5112.365 kb on - strand, within Pf6N2E2_4384 at 5112.546 kb on + strand, within Pf6N2E2_4384 at 5112.547 kb on - strand, within Pf6N2E2_4384 at 5112.547 kb on - strand, within Pf6N2E2_4384 at 5112.607 kb on - strand, within Pf6N2E2_4384 at 5112.649 kb on + strand, within Pf6N2E2_4384 at 5112.649 kb on + strand, within Pf6N2E2_4384 at 5112.650 kb on - strand, within Pf6N2E2_4384 at 5112.698 kb on + strand, within Pf6N2E2_4384 at 5112.699 kb on - strand, within Pf6N2E2_4384 at 5112.699 kb on - strand, within Pf6N2E2_4384 at 5112.699 kb on - strand, within Pf6N2E2_4384 at 5112.804 kb on + strand, within Pf6N2E2_4384 at 5112.804 kb on + strand, within Pf6N2E2_4384 at 5112.805 kb on - strand, within Pf6N2E2_4384 at 5112.805 kb on - strand, within Pf6N2E2_4384 at 5112.835 kb on + strand, within Pf6N2E2_4384 at 5112.835 kb on + strand, within Pf6N2E2_4384 at 5112.835 kb on + strand, within Pf6N2E2_4384 at 5112.835 kb on + strand, within Pf6N2E2_4384 at 5112.836 kb on - strand, within Pf6N2E2_4384 at 5112.836 kb on - strand, within Pf6N2E2_4384 at 5112.958 kb on + strand, within Pf6N2E2_4384 at 5113.015 kb on + strand, within Pf6N2E2_4384 at 5113.016 kb on - strand, within Pf6N2E2_4384 at 5113.404 kb on - strand at 5113.404 kb on - strand at 5113.547 kb on + strand at 5113.547 kb on + strand at 5113.548 kb on - strand at 5113.548 kb on - strand at 5113.548 kb on - strand at 5113.548 kb on - strand at 5113.751 kb on + strand, within Pf6N2E2_4385 at 5113.751 kb on + strand, within Pf6N2E2_4385 at 5113.751 kb on + strand, within Pf6N2E2_4385 at 5113.751 kb on + strand, within Pf6N2E2_4385 at 5113.751 kb on + strand, within Pf6N2E2_4385 at 5113.751 kb on + strand, within Pf6N2E2_4385 at 5113.752 kb on - strand, within Pf6N2E2_4385 at 5113.752 kb on - strand, within Pf6N2E2_4385 at 5113.752 kb on - strand, within Pf6N2E2_4385 at 5113.752 kb on - strand, within Pf6N2E2_4385 at 5113.937 kb on + strand, within Pf6N2E2_4385 at 5113.937 kb on + strand, within Pf6N2E2_4385 at 5113.937 kb on + strand, within Pf6N2E2_4385 at 5113.937 kb on + strand, within Pf6N2E2_4385 at 5113.937 kb on + strand, within Pf6N2E2_4385 at 5113.938 kb on - strand, within Pf6N2E2_4385 at 5113.938 kb on - strand, within Pf6N2E2_4385 at 5113.938 kb on - strand, within Pf6N2E2_4385 at 5113.938 kb on - strand, within Pf6N2E2_4385 at 5113.967 kb on + strand, within Pf6N2E2_4385 at 5114.168 kb on + strand, within Pf6N2E2_4385 at 5114.169 kb on - strand, within Pf6N2E2_4385
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with copper (II) chloride 0.03 mM remove 5,111,162 + Pf6N2E2_4383 0.47 +0.9 5,111,162 + Pf6N2E2_4383 0.47 -1.1 5,111,163 - Pf6N2E2_4383 0.47 -0.2 5,111,163 - Pf6N2E2_4383 0.47 +0.8 5,111,163 - Pf6N2E2_4383 0.47 -1.3 5,111,174 + Pf6N2E2_4383 0.48 -1.0 5,111,223 + Pf6N2E2_4383 0.51 +0.9 5,111,224 - Pf6N2E2_4383 0.51 -0.5 5,111,224 - Pf6N2E2_4383 0.51 -1.2 5,111,224 - Pf6N2E2_4383 0.51 -0.4 5,111,224 - Pf6N2E2_4383 0.51 -0.2 5,111,224 - Pf6N2E2_4383 0.51 +0.6 5,111,249 + Pf6N2E2_4383 0.53 -0.8 5,111,249 + Pf6N2E2_4383 0.53 +0.4 5,111,249 + Pf6N2E2_4383 0.53 +0.4 5,111,249 + Pf6N2E2_4383 0.53 -1.3 5,111,250 - Pf6N2E2_4383 0.53 -0.8 5,111,526 + Pf6N2E2_4383 0.71 +0.8 5,111,526 + Pf6N2E2_4383 0.71 -0.1 5,111,527 - Pf6N2E2_4383 0.71 -0.0 5,111,600 + Pf6N2E2_4383 0.76 +0.1 5,111,600 + Pf6N2E2_4383 0.76 -1.9 5,111,601 - Pf6N2E2_4383 0.76 +0.5 5,111,601 - Pf6N2E2_4383 0.76 +1.6 5,111,604 + Pf6N2E2_4383 0.77 +0.3 5,111,604 + Pf6N2E2_4383 0.77 +0.4 5,111,604 + Pf6N2E2_4383 0.77 -2.3 5,111,604 + Pf6N2E2_4383 0.77 -1.6 5,111,604 + Pf6N2E2_4383 0.77 +1.3 5,111,604 + Pf6N2E2_4383 0.77 +0.0 5,111,604 + Pf6N2E2_4383 0.77 +0.5 5,111,604 + Pf6N2E2_4383 0.77 +1.2 5,111,605 - Pf6N2E2_4383 0.77 +0.1 5,111,605 - Pf6N2E2_4383 0.77 +1.8 5,111,605 - Pf6N2E2_4383 0.77 -2.2 5,111,605 - Pf6N2E2_4383 0.77 -0.6 5,111,605 - Pf6N2E2_4383 0.77 -1.2 5,111,605 - Pf6N2E2_4383 0.77 +0.4 5,111,771 + Pf6N2E2_4383 0.88 -0.9 5,111,771 + Pf6N2E2_4383 0.88 -1.2 5,111,772 - Pf6N2E2_4383 0.88 +0.8 5,111,832 + -0.7 5,111,832 + -0.2 5,111,832 + -1.8 5,111,920 - +0.5 5,111,920 - +1.4 5,111,920 - -0.0 5,111,920 - +0.7 5,111,920 - +0.5 5,111,920 - +0.4 5,111,920 - +0.4 5,111,984 + +0.4 5,112,028 + +0.4 5,112,029 - +0.6 5,112,115 + +0.9 5,112,115 + +0.0 5,112,115 + +0.1 5,112,115 + -1.4 5,112,115 + -0.3 5,112,116 - +0.9 5,112,116 - -1.8 5,112,116 - -0.5 5,112,116 - -2.7 5,112,116 - +1.0 5,112,116 - -0.1 5,112,153 + +1.2 5,112,153 + +0.5 5,112,154 - -0.3 5,112,154 - -0.0 5,112,244 - +1.1 5,112,364 + Pf6N2E2_4384 0.20 -1.0 5,112,365 - Pf6N2E2_4384 0.20 -1.4 5,112,546 + Pf6N2E2_4384 0.36 +0.1 5,112,547 - Pf6N2E2_4384 0.36 -1.0 5,112,547 - Pf6N2E2_4384 0.36 -0.7 5,112,607 - Pf6N2E2_4384 0.41 -1.0 5,112,649 + Pf6N2E2_4384 0.45 -1.3 5,112,649 + Pf6N2E2_4384 0.45 +1.5 5,112,650 - Pf6N2E2_4384 0.45 -0.4 5,112,698 + Pf6N2E2_4384 0.49 +0.9 5,112,699 - Pf6N2E2_4384 0.49 +0.3 5,112,699 - Pf6N2E2_4384 0.49 +0.9 5,112,699 - Pf6N2E2_4384 0.49 +1.4 5,112,804 + Pf6N2E2_4384 0.58 +0.0 5,112,804 + Pf6N2E2_4384 0.58 +0.0 5,112,805 - Pf6N2E2_4384 0.58 -0.7 5,112,805 - Pf6N2E2_4384 0.58 -1.6 5,112,835 + Pf6N2E2_4384 0.61 +0.1 5,112,835 + Pf6N2E2_4384 0.61 +1.0 5,112,835 + Pf6N2E2_4384 0.61 +1.0 5,112,835 + Pf6N2E2_4384 0.61 -0.4 5,112,836 - Pf6N2E2_4384 0.61 -0.8 5,112,836 - Pf6N2E2_4384 0.61 +1.4 5,112,958 + Pf6N2E2_4384 0.72 +0.8 5,113,015 + Pf6N2E2_4384 0.77 +1.8 5,113,016 - Pf6N2E2_4384 0.77 +0.8 5,113,404 - +0.8 5,113,404 - -0.6 5,113,547 + +0.4 5,113,547 + +1.1 5,113,548 - -0.2 5,113,548 - +1.1 5,113,548 - -1.9 5,113,548 - +0.3 5,113,751 + Pf6N2E2_4385 0.22 -0.4 5,113,751 + Pf6N2E2_4385 0.22 -3.8 5,113,751 + Pf6N2E2_4385 0.22 -0.5 5,113,751 + Pf6N2E2_4385 0.22 +0.4 5,113,751 + Pf6N2E2_4385 0.22 +0.3 5,113,751 + Pf6N2E2_4385 0.22 +0.9 5,113,752 - Pf6N2E2_4385 0.22 +0.2 5,113,752 - Pf6N2E2_4385 0.22 +0.3 5,113,752 - Pf6N2E2_4385 0.22 -1.1 5,113,752 - Pf6N2E2_4385 0.22 -1.9 5,113,937 + Pf6N2E2_4385 0.34 -0.7 5,113,937 + Pf6N2E2_4385 0.34 -0.6 5,113,937 + Pf6N2E2_4385 0.34 +1.0 5,113,937 + Pf6N2E2_4385 0.34 -0.1 5,113,937 + Pf6N2E2_4385 0.34 +0.4 5,113,938 - Pf6N2E2_4385 0.34 -1.2 5,113,938 - Pf6N2E2_4385 0.34 +1.2 5,113,938 - Pf6N2E2_4385 0.34 +0.6 5,113,938 - Pf6N2E2_4385 0.34 -0.3 5,113,967 + Pf6N2E2_4385 0.36 -2.4 5,114,168 + Pf6N2E2_4385 0.49 +0.3 5,114,169 - Pf6N2E2_4385 0.49 +0.0
Or see this region's nucleotide sequence