Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5861 and Pf6N2E2_5862 are separated by 72 nucleotides Pf6N2E2_5862 and Pf6N2E2_5863 are separated by 157 nucleotides Pf6N2E2_5863 and Pf6N2E2_5864 overlap by 4 nucleotides Pf6N2E2_5864 and Pf6N2E2_5865 are separated by 81 nucleotides
Pf6N2E2_5861 - FIG00953885: hypothetical protein, at 6,681,686 to 6,682,423
_5861
Pf6N2E2_5862 - Lipoprotein, putative, at 6,682,496 to 6,682,744
_5862
Pf6N2E2_5863 - FIG00956080: hypothetical protein, at 6,682,902 to 6,683,378
_5863
Pf6N2E2_5864 - FIG00953296: hypothetical protein, at 6,683,375 to 6,683,836
_5864
Pf6N2E2_5865 - Chaperone protein HtpG, at 6,683,918 to 6,685,828
_5865
Position (kb)
6683
6684 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6682.375 kb on + strand at 6682.376 kb on - strand at 6682.487 kb on - strand at 6682.494 kb on + strand at 6682.495 kb on - strand at 6682.603 kb on + strand, within Pf6N2E2_5862 at 6682.604 kb on - strand, within Pf6N2E2_5862 at 6682.738 kb on + strand at 6682.771 kb on + strand at 6682.772 kb on - strand at 6682.772 kb on - strand at 6682.802 kb on - strand at 6682.823 kb on + strand at 6682.823 kb on + strand at 6682.823 kb on + strand at 6682.823 kb on + strand at 6682.824 kb on - strand at 6682.824 kb on - strand at 6682.824 kb on - strand at 6682.824 kb on - strand at 6682.825 kb on + strand at 6682.826 kb on - strand at 6682.826 kb on - strand at 6682.826 kb on - strand at 6682.826 kb on - strand at 6682.826 kb on - strand at 6682.827 kb on + strand at 6682.827 kb on + strand at 6682.827 kb on + strand at 6682.827 kb on + strand at 6682.827 kb on + strand at 6682.828 kb on - strand at 6682.828 kb on - strand at 6682.828 kb on - strand at 6682.828 kb on - strand at 6682.828 kb on - strand at 6682.828 kb on - strand at 6683.105 kb on + strand, within Pf6N2E2_5863 at 6683.105 kb on + strand, within Pf6N2E2_5863 at 6683.105 kb on + strand, within Pf6N2E2_5863 at 6683.106 kb on - strand, within Pf6N2E2_5863 at 6683.106 kb on - strand, within Pf6N2E2_5863 at 6683.169 kb on + strand, within Pf6N2E2_5863 at 6683.169 kb on + strand, within Pf6N2E2_5863 at 6683.169 kb on + strand, within Pf6N2E2_5863 at 6683.169 kb on + strand, within Pf6N2E2_5863 at 6683.170 kb on - strand, within Pf6N2E2_5863 at 6683.170 kb on - strand, within Pf6N2E2_5863 at 6683.203 kb on - strand, within Pf6N2E2_5863 at 6683.427 kb on - strand, within Pf6N2E2_5864 at 6683.453 kb on + strand, within Pf6N2E2_5864 at 6683.454 kb on - strand, within Pf6N2E2_5864 at 6683.536 kb on + strand, within Pf6N2E2_5864 at 6683.545 kb on - strand, within Pf6N2E2_5864 at 6683.663 kb on + strand, within Pf6N2E2_5864 at 6683.664 kb on - strand, within Pf6N2E2_5864 at 6683.693 kb on + strand, within Pf6N2E2_5864 at 6683.693 kb on + strand, within Pf6N2E2_5864 at 6683.693 kb on + strand, within Pf6N2E2_5864 at 6683.694 kb on - strand, within Pf6N2E2_5864 at 6683.694 kb on - strand, within Pf6N2E2_5864 at 6683.694 kb on - strand, within Pf6N2E2_5864 at 6684.070 kb on - strand at 6684.689 kb on + strand, within Pf6N2E2_5865 at 6684.689 kb on + strand, within Pf6N2E2_5865 at 6684.689 kb on + strand, within Pf6N2E2_5865 at 6684.689 kb on + strand, within Pf6N2E2_5865 at 6684.690 kb on - strand, within Pf6N2E2_5865 at 6684.761 kb on + strand, within Pf6N2E2_5865 at 6684.762 kb on - strand, within Pf6N2E2_5865 at 6684.762 kb on - strand, within Pf6N2E2_5865 at 6684.762 kb on - strand, within Pf6N2E2_5865 at 6684.776 kb on + strand, within Pf6N2E2_5865 at 6684.776 kb on + strand, within Pf6N2E2_5865 at 6684.776 kb on + strand, within Pf6N2E2_5865 at 6684.806 kb on + strand, within Pf6N2E2_5865
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 6,682,375 + +0.8 6,682,376 - -1.3 6,682,487 - -0.5 6,682,494 + +1.4 6,682,495 - +1.4 6,682,603 + Pf6N2E2_5862 0.43 -1.6 6,682,604 - Pf6N2E2_5862 0.43 -1.1 6,682,738 + -0.3 6,682,771 + +1.4 6,682,772 - -0.1 6,682,772 - -0.2 6,682,802 - +0.3 6,682,823 + -0.5 6,682,823 + -1.3 6,682,823 + -1.0 6,682,823 + -0.4 6,682,824 - +2.4 6,682,824 - -1.6 6,682,824 - -0.6 6,682,824 - +0.4 6,682,825 + -1.3 6,682,826 - +0.7 6,682,826 - -0.2 6,682,826 - +1.6 6,682,826 - -0.8 6,682,826 - -0.4 6,682,827 + -0.5 6,682,827 + -1.4 6,682,827 + +0.8 6,682,827 + -1.3 6,682,827 + +1.8 6,682,828 - -2.2 6,682,828 - +2.4 6,682,828 - -0.6 6,682,828 - +0.3 6,682,828 - -2.0 6,682,828 - -0.7 6,683,105 + Pf6N2E2_5863 0.43 +0.2 6,683,105 + Pf6N2E2_5863 0.43 -0.0 6,683,105 + Pf6N2E2_5863 0.43 -1.4 6,683,106 - Pf6N2E2_5863 0.43 +0.3 6,683,106 - Pf6N2E2_5863 0.43 +1.0 6,683,169 + Pf6N2E2_5863 0.56 -0.1 6,683,169 + Pf6N2E2_5863 0.56 +2.1 6,683,169 + Pf6N2E2_5863 0.56 +0.2 6,683,169 + Pf6N2E2_5863 0.56 +1.0 6,683,170 - Pf6N2E2_5863 0.56 +1.6 6,683,170 - Pf6N2E2_5863 0.56 +0.2 6,683,203 - Pf6N2E2_5863 0.63 +0.2 6,683,427 - Pf6N2E2_5864 0.11 -0.2 6,683,453 + Pf6N2E2_5864 0.17 -0.6 6,683,454 - Pf6N2E2_5864 0.17 -2.6 6,683,536 + Pf6N2E2_5864 0.35 -1.0 6,683,545 - Pf6N2E2_5864 0.37 -2.2 6,683,663 + Pf6N2E2_5864 0.62 -1.6 6,683,664 - Pf6N2E2_5864 0.63 -0.3 6,683,693 + Pf6N2E2_5864 0.69 +2.2 6,683,693 + Pf6N2E2_5864 0.69 -0.6 6,683,693 + Pf6N2E2_5864 0.69 +0.0 6,683,694 - Pf6N2E2_5864 0.69 -0.2 6,683,694 - Pf6N2E2_5864 0.69 -3.0 6,683,694 - Pf6N2E2_5864 0.69 -2.2 6,684,070 - -1.0 6,684,689 + Pf6N2E2_5865 0.40 +1.0 6,684,689 + Pf6N2E2_5865 0.40 +1.3 6,684,689 + Pf6N2E2_5865 0.40 -1.0 6,684,689 + Pf6N2E2_5865 0.40 +2.7 6,684,690 - Pf6N2E2_5865 0.40 +0.0 6,684,761 + Pf6N2E2_5865 0.44 +0.0 6,684,762 - Pf6N2E2_5865 0.44 -0.3 6,684,762 - Pf6N2E2_5865 0.44 +1.4 6,684,762 - Pf6N2E2_5865 0.44 -1.8 6,684,776 + Pf6N2E2_5865 0.45 -1.2 6,684,776 + Pf6N2E2_5865 0.45 -0.2 6,684,776 + Pf6N2E2_5865 0.45 +1.7 6,684,806 + Pf6N2E2_5865 0.46 +1.9
Or see this region's nucleotide sequence