Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2718 and Pf6N2E2_2719 are separated by 77 nucleotides Pf6N2E2_2719 and Pf6N2E2_2720 are separated by 184 nucleotides Pf6N2E2_2720 and Pf6N2E2_2721 are separated by 77 nucleotides Pf6N2E2_2721 and Pf6N2E2_2722 are separated by 102 nucleotides
Pf6N2E2_2718 - Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4), at 3,210,295 to 3,212,454
_2718
Pf6N2E2_2719 - FIG00961680: hypothetical protein, at 3,212,532 to 3,212,864
_2719
Pf6N2E2_2720 - tRNA (guanosine(18)-2'-O)-methyltransferase (EC 2.1.1.34), at 3,213,049 to 3,213,519
_2720
Pf6N2E2_2721 - FIG00954302: hypothetical protein, at 3,213,597 to 3,213,974
_2721
Pf6N2E2_2722 - FIG00954807: hypothetical protein, at 3,214,077 to 3,215,285
_2722
Position (kb)
3213
3214 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3212.150 kb on + strand, within Pf6N2E2_2718 at 3212.150 kb on + strand, within Pf6N2E2_2718 at 3212.166 kb on + strand, within Pf6N2E2_2718 at 3212.167 kb on - strand, within Pf6N2E2_2718 at 3212.210 kb on + strand, within Pf6N2E2_2718 at 3212.211 kb on - strand, within Pf6N2E2_2718 at 3212.265 kb on + strand at 3212.302 kb on + strand at 3212.302 kb on + strand at 3212.303 kb on - strand at 3212.303 kb on - strand at 3212.304 kb on + strand at 3212.304 kb on + strand at 3212.304 kb on + strand at 3212.304 kb on + strand at 3212.305 kb on - strand at 3212.317 kb on - strand at 3212.436 kb on + strand at 3212.452 kb on + strand at 3212.453 kb on - strand at 3212.453 kb on - strand at 3212.553 kb on - strand at 3212.553 kb on - strand at 3212.567 kb on + strand, within Pf6N2E2_2719 at 3212.567 kb on + strand, within Pf6N2E2_2719 at 3212.568 kb on - strand, within Pf6N2E2_2719 at 3212.568 kb on - strand, within Pf6N2E2_2719 at 3212.568 kb on - strand, within Pf6N2E2_2719 at 3212.568 kb on - strand, within Pf6N2E2_2719 at 3212.568 kb on - strand, within Pf6N2E2_2719 at 3212.568 kb on - strand, within Pf6N2E2_2719 at 3212.568 kb on - strand, within Pf6N2E2_2719 at 3212.569 kb on + strand, within Pf6N2E2_2719 at 3212.569 kb on + strand, within Pf6N2E2_2719 at 3212.570 kb on - strand, within Pf6N2E2_2719 at 3212.570 kb on - strand, within Pf6N2E2_2719 at 3212.605 kb on + strand, within Pf6N2E2_2719 at 3212.605 kb on + strand, within Pf6N2E2_2719 at 3212.605 kb on + strand, within Pf6N2E2_2719 at 3212.605 kb on + strand, within Pf6N2E2_2719 at 3212.605 kb on + strand, within Pf6N2E2_2719 at 3212.606 kb on - strand, within Pf6N2E2_2719 at 3212.640 kb on - strand, within Pf6N2E2_2719 at 3212.652 kb on - strand, within Pf6N2E2_2719 at 3212.652 kb on - strand, within Pf6N2E2_2719 at 3212.670 kb on - strand, within Pf6N2E2_2719 at 3212.689 kb on + strand, within Pf6N2E2_2719 at 3212.696 kb on - strand, within Pf6N2E2_2719 at 3212.737 kb on + strand, within Pf6N2E2_2719 at 3212.737 kb on + strand, within Pf6N2E2_2719 at 3212.738 kb on - strand, within Pf6N2E2_2719 at 3213.014 kb on + strand at 3213.014 kb on + strand at 3213.017 kb on - strand at 3213.017 kb on - strand at 3213.064 kb on + strand at 3213.065 kb on - strand at 3213.065 kb on - strand at 3213.066 kb on + strand at 3213.066 kb on + strand at 3213.066 kb on + strand at 3213.066 kb on + strand at 3213.067 kb on - strand at 3213.067 kb on - strand at 3213.136 kb on + strand, within Pf6N2E2_2720 at 3213.157 kb on + strand, within Pf6N2E2_2720 at 3213.157 kb on + strand, within Pf6N2E2_2720 at 3213.158 kb on - strand, within Pf6N2E2_2720 at 3213.158 kb on - strand, within Pf6N2E2_2720 at 3213.172 kb on + strand, within Pf6N2E2_2720 at 3213.173 kb on - strand, within Pf6N2E2_2720 at 3213.217 kb on + strand, within Pf6N2E2_2720 at 3213.217 kb on + strand, within Pf6N2E2_2720 at 3213.218 kb on - strand, within Pf6N2E2_2720 at 3213.322 kb on + strand, within Pf6N2E2_2720 at 3213.322 kb on + strand, within Pf6N2E2_2720 at 3213.322 kb on + strand, within Pf6N2E2_2720 at 3213.323 kb on - strand, within Pf6N2E2_2720 at 3213.323 kb on - strand, within Pf6N2E2_2720 at 3213.323 kb on - strand, within Pf6N2E2_2720 at 3213.412 kb on + strand, within Pf6N2E2_2720 at 3213.412 kb on + strand, within Pf6N2E2_2720 at 3213.412 kb on + strand, within Pf6N2E2_2720 at 3213.412 kb on + strand, within Pf6N2E2_2720 at 3213.412 kb on + strand, within Pf6N2E2_2720 at 3213.412 kb on + strand, within Pf6N2E2_2720 at 3213.413 kb on - strand, within Pf6N2E2_2720 at 3213.413 kb on - strand, within Pf6N2E2_2720 at 3213.413 kb on - strand, within Pf6N2E2_2720 at 3213.413 kb on - strand, within Pf6N2E2_2720 at 3213.413 kb on - strand, within Pf6N2E2_2720 at 3213.413 kb on - strand, within Pf6N2E2_2720 at 3213.413 kb on - strand, within Pf6N2E2_2720 at 3213.413 kb on - strand, within Pf6N2E2_2720 at 3213.466 kb on + strand, within Pf6N2E2_2720 at 3213.466 kb on + strand, within Pf6N2E2_2720 at 3213.467 kb on - strand, within Pf6N2E2_2720 at 3213.467 kb on - strand, within Pf6N2E2_2720 at 3213.467 kb on - strand, within Pf6N2E2_2720 at 3213.568 kb on + strand at 3213.568 kb on + strand at 3213.568 kb on + strand at 3213.568 kb on + strand at 3213.568 kb on + strand at 3213.568 kb on + strand at 3213.569 kb on - strand at 3213.570 kb on + strand at 3213.570 kb on + strand at 3213.570 kb on + strand at 3213.570 kb on + strand at 3213.570 kb on + strand at 3213.571 kb on - strand at 3213.571 kb on - strand at 3213.571 kb on - strand at 3213.582 kb on + strand at 3213.582 kb on + strand at 3213.583 kb on - strand at 3213.605 kb on + strand at 3214.106 kb on + strand at 3214.106 kb on + strand at 3214.107 kb on - strand at 3214.352 kb on - strand, within Pf6N2E2_2722 at 3214.399 kb on + strand, within Pf6N2E2_2722 at 3214.399 kb on + strand, within Pf6N2E2_2722 at 3214.400 kb on - strand, within Pf6N2E2_2722
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 3,212,150 + Pf6N2E2_2718 0.86 +0.3 3,212,150 + Pf6N2E2_2718 0.86 -4.4 3,212,166 + Pf6N2E2_2718 0.87 +0.8 3,212,167 - Pf6N2E2_2718 0.87 +1.4 3,212,210 + Pf6N2E2_2718 0.89 -0.5 3,212,211 - Pf6N2E2_2718 0.89 -0.0 3,212,265 + -1.9 3,212,302 + -1.2 3,212,302 + -0.6 3,212,303 - +1.8 3,212,303 - -0.4 3,212,304 + -0.4 3,212,304 + +0.4 3,212,304 + +1.0 3,212,304 + +1.0 3,212,305 - -1.0 3,212,317 - -2.2 3,212,436 + +0.1 3,212,452 + -0.6 3,212,453 - +0.7 3,212,453 - -2.0 3,212,553 - -1.6 3,212,553 - -1.5 3,212,567 + Pf6N2E2_2719 0.11 +0.3 3,212,567 + Pf6N2E2_2719 0.11 +1.0 3,212,568 - Pf6N2E2_2719 0.11 -2.4 3,212,568 - Pf6N2E2_2719 0.11 -0.0 3,212,568 - Pf6N2E2_2719 0.11 -1.0 3,212,568 - Pf6N2E2_2719 0.11 -0.6 3,212,568 - Pf6N2E2_2719 0.11 -0.6 3,212,568 - Pf6N2E2_2719 0.11 +0.1 3,212,568 - Pf6N2E2_2719 0.11 -0.0 3,212,569 + Pf6N2E2_2719 0.11 +0.3 3,212,569 + Pf6N2E2_2719 0.11 +0.5 3,212,570 - Pf6N2E2_2719 0.11 -1.0 3,212,570 - Pf6N2E2_2719 0.11 -1.0 3,212,605 + Pf6N2E2_2719 0.22 +1.0 3,212,605 + Pf6N2E2_2719 0.22 +1.0 3,212,605 + Pf6N2E2_2719 0.22 +0.5 3,212,605 + Pf6N2E2_2719 0.22 -0.6 3,212,605 + Pf6N2E2_2719 0.22 +1.7 3,212,606 - Pf6N2E2_2719 0.22 +1.0 3,212,640 - Pf6N2E2_2719 0.32 +1.5 3,212,652 - Pf6N2E2_2719 0.36 -1.1 3,212,652 - Pf6N2E2_2719 0.36 -0.8 3,212,670 - Pf6N2E2_2719 0.41 +1.3 3,212,689 + Pf6N2E2_2719 0.47 -1.2 3,212,696 - Pf6N2E2_2719 0.49 -0.4 3,212,737 + Pf6N2E2_2719 0.62 -1.2 3,212,737 + Pf6N2E2_2719 0.62 -2.4 3,212,738 - Pf6N2E2_2719 0.62 +0.1 3,213,014 + +0.9 3,213,014 + -1.0 3,213,017 - +1.3 3,213,017 - -1.4 3,213,064 + -2.0 3,213,065 - +0.4 3,213,065 - +0.3 3,213,066 + +1.0 3,213,066 + +1.0 3,213,066 + +0.4 3,213,066 + -1.9 3,213,067 - -1.2 3,213,067 - +0.3 3,213,136 + Pf6N2E2_2720 0.18 +1.0 3,213,157 + Pf6N2E2_2720 0.23 +1.4 3,213,157 + Pf6N2E2_2720 0.23 +0.6 3,213,158 - Pf6N2E2_2720 0.23 -0.4 3,213,158 - Pf6N2E2_2720 0.23 +1.3 3,213,172 + Pf6N2E2_2720 0.26 -0.6 3,213,173 - Pf6N2E2_2720 0.26 -0.6 3,213,217 + Pf6N2E2_2720 0.36 -2.0 3,213,217 + Pf6N2E2_2720 0.36 -0.0 3,213,218 - Pf6N2E2_2720 0.36 +0.3 3,213,322 + Pf6N2E2_2720 0.58 -0.4 3,213,322 + Pf6N2E2_2720 0.58 +0.4 3,213,322 + Pf6N2E2_2720 0.58 +2.1 3,213,323 - Pf6N2E2_2720 0.58 -1.4 3,213,323 - Pf6N2E2_2720 0.58 +1.1 3,213,323 - Pf6N2E2_2720 0.58 +0.7 3,213,412 + Pf6N2E2_2720 0.77 +1.2 3,213,412 + Pf6N2E2_2720 0.77 +0.3 3,213,412 + Pf6N2E2_2720 0.77 -0.3 3,213,412 + Pf6N2E2_2720 0.77 +1.5 3,213,412 + Pf6N2E2_2720 0.77 -1.4 3,213,412 + Pf6N2E2_2720 0.77 +0.5 3,213,413 - Pf6N2E2_2720 0.77 +3.3 3,213,413 - Pf6N2E2_2720 0.77 -1.0 3,213,413 - Pf6N2E2_2720 0.77 -3.0 3,213,413 - Pf6N2E2_2720 0.77 +0.2 3,213,413 - Pf6N2E2_2720 0.77 +2.3 3,213,413 - Pf6N2E2_2720 0.77 +1.0 3,213,413 - Pf6N2E2_2720 0.77 +1.6 3,213,413 - Pf6N2E2_2720 0.77 -0.6 3,213,466 + Pf6N2E2_2720 0.89 +0.3 3,213,466 + Pf6N2E2_2720 0.89 +2.1 3,213,467 - Pf6N2E2_2720 0.89 +1.3 3,213,467 - Pf6N2E2_2720 0.89 +0.7 3,213,467 - Pf6N2E2_2720 0.89 -1.0 3,213,568 + +0.9 3,213,568 + +0.6 3,213,568 + -0.2 3,213,568 + +0.5 3,213,568 + -0.0 3,213,568 + -0.8 3,213,569 - +0.6 3,213,570 + +1.0 3,213,570 + -0.2 3,213,570 + -2.4 3,213,570 + +0.4 3,213,570 + +0.1 3,213,571 - +0.1 3,213,571 - -1.4 3,213,571 - +0.1 3,213,582 + -1.5 3,213,582 + -2.6 3,213,583 - +0.5 3,213,605 + +0.3 3,214,106 + +1.0 3,214,106 + -1.0 3,214,107 - -1.4 3,214,352 - Pf6N2E2_2722 0.23 -1.4 3,214,399 + Pf6N2E2_2722 0.27 -1.9 3,214,399 + Pf6N2E2_2722 0.27 -1.6 3,214,400 - Pf6N2E2_2722 0.27 -1.8
Or see this region's nucleotide sequence