Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1106 and Pf6N2E2_1107 are separated by 128 nucleotides Pf6N2E2_1107 and Pf6N2E2_1108 are separated by 3 nucleotides
Pf6N2E2_1106 - Chromosome initiation inhibitor, at 1,293,205 to 1,294,134
_1106
Pf6N2E2_1107 - hypothetical protein, at 1,294,263 to 1,299,359
_1107
Pf6N2E2_1108 - Methyltransferase (EC 2.1.1.-), at 1,299,363 to 1,300,421
_1108
Position (kb)
1294
1295
1296
1297
1298
1299
1300 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1293.437 kb on + strand, within Pf6N2E2_1106 at 1293.437 kb on + strand, within Pf6N2E2_1106 at 1293.496 kb on + strand, within Pf6N2E2_1106 at 1293.698 kb on - strand, within Pf6N2E2_1106 at 1293.913 kb on + strand, within Pf6N2E2_1106 at 1293.914 kb on - strand, within Pf6N2E2_1106 at 1293.914 kb on - strand, within Pf6N2E2_1106 at 1294.028 kb on + strand, within Pf6N2E2_1106 at 1294.028 kb on + strand, within Pf6N2E2_1106 at 1294.029 kb on - strand, within Pf6N2E2_1106 at 1294.029 kb on - strand, within Pf6N2E2_1106 at 1294.199 kb on - strand at 1294.199 kb on - strand at 1294.372 kb on + strand at 1294.372 kb on + strand at 1294.372 kb on + strand at 1294.373 kb on - strand at 1294.373 kb on - strand at 1294.373 kb on - strand at 1294.373 kb on - strand at 1294.373 kb on - strand at 1294.373 kb on - strand at 1294.434 kb on - strand at 1294.584 kb on + strand at 1294.585 kb on - strand at 1294.585 kb on - strand at 1294.824 kb on + strand, within Pf6N2E2_1107 at 1294.824 kb on + strand, within Pf6N2E2_1107 at 1294.825 kb on - strand, within Pf6N2E2_1107 at 1294.825 kb on - strand, within Pf6N2E2_1107 at 1294.825 kb on - strand, within Pf6N2E2_1107 at 1294.833 kb on + strand, within Pf6N2E2_1107 at 1294.833 kb on + strand, within Pf6N2E2_1107 at 1294.834 kb on - strand, within Pf6N2E2_1107 at 1294.834 kb on - strand, within Pf6N2E2_1107 at 1294.917 kb on + strand, within Pf6N2E2_1107 at 1294.918 kb on - strand, within Pf6N2E2_1107 at 1295.047 kb on + strand, within Pf6N2E2_1107 at 1295.242 kb on + strand, within Pf6N2E2_1107 at 1295.242 kb on + strand, within Pf6N2E2_1107 at 1295.423 kb on + strand, within Pf6N2E2_1107 at 1295.607 kb on + strand, within Pf6N2E2_1107 at 1295.608 kb on - strand, within Pf6N2E2_1107 at 1295.624 kb on + strand, within Pf6N2E2_1107 at 1295.625 kb on - strand, within Pf6N2E2_1107 at 1295.669 kb on + strand, within Pf6N2E2_1107 at 1295.669 kb on + strand, within Pf6N2E2_1107 at 1295.669 kb on + strand, within Pf6N2E2_1107 at 1295.886 kb on + strand, within Pf6N2E2_1107 at 1296.526 kb on - strand, within Pf6N2E2_1107 at 1296.838 kb on + strand, within Pf6N2E2_1107 at 1296.838 kb on + strand, within Pf6N2E2_1107 at 1297.180 kb on - strand, within Pf6N2E2_1107 at 1297.447 kb on - strand, within Pf6N2E2_1107 at 1297.447 kb on - strand, within Pf6N2E2_1107 at 1297.683 kb on - strand, within Pf6N2E2_1107 at 1298.009 kb on - strand, within Pf6N2E2_1107 at 1298.009 kb on - strand, within Pf6N2E2_1107 at 1298.095 kb on + strand, within Pf6N2E2_1107 at 1298.322 kb on - strand, within Pf6N2E2_1107 at 1298.322 kb on - strand, within Pf6N2E2_1107 at 1298.345 kb on + strand, within Pf6N2E2_1107 at 1298.345 kb on + strand, within Pf6N2E2_1107 at 1298.346 kb on - strand, within Pf6N2E2_1107 at 1298.407 kb on - strand, within Pf6N2E2_1107 at 1298.589 kb on + strand, within Pf6N2E2_1107 at 1298.589 kb on + strand, within Pf6N2E2_1107 at 1298.590 kb on - strand, within Pf6N2E2_1107 at 1298.590 kb on - strand, within Pf6N2E2_1107 at 1298.592 kb on + strand, within Pf6N2E2_1107 at 1298.592 kb on + strand, within Pf6N2E2_1107 at 1298.592 kb on + strand, within Pf6N2E2_1107 at 1298.593 kb on - strand, within Pf6N2E2_1107 at 1298.613 kb on + strand, within Pf6N2E2_1107 at 1298.613 kb on + strand, within Pf6N2E2_1107 at 1298.613 kb on + strand, within Pf6N2E2_1107 at 1298.614 kb on - strand, within Pf6N2E2_1107 at 1298.614 kb on - strand, within Pf6N2E2_1107 at 1298.614 kb on - strand, within Pf6N2E2_1107 at 1298.614 kb on - strand, within Pf6N2E2_1107 at 1298.614 kb on - strand, within Pf6N2E2_1107 at 1298.614 kb on - strand, within Pf6N2E2_1107 at 1298.614 kb on - strand, within Pf6N2E2_1107 at 1298.818 kb on - strand, within Pf6N2E2_1107 at 1298.831 kb on - strand, within Pf6N2E2_1107 at 1299.088 kb on + strand at 1299.267 kb on + strand at 1299.267 kb on + strand at 1299.267 kb on + strand at 1299.267 kb on + strand at 1299.267 kb on + strand at 1299.267 kb on + strand at 1299.268 kb on - strand at 1299.268 kb on - strand at 1299.268 kb on - strand at 1299.268 kb on - strand at 1299.268 kb on - strand at 1299.357 kb on + strand at 1299.667 kb on + strand, within Pf6N2E2_1108 at 1300.049 kb on - strand, within Pf6N2E2_1108 at 1300.059 kb on + strand, within Pf6N2E2_1108 at 1300.070 kb on + strand, within Pf6N2E2_1108 at 1300.103 kb on + strand, within Pf6N2E2_1108 at 1300.103 kb on + strand, within Pf6N2E2_1108 at 1300.103 kb on + strand, within Pf6N2E2_1108 at 1300.104 kb on - strand, within Pf6N2E2_1108 at 1300.359 kb on + strand at 1300.359 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 1,293,437 + Pf6N2E2_1106 0.25 -0.4 1,293,437 + Pf6N2E2_1106 0.25 -1.6 1,293,496 + Pf6N2E2_1106 0.31 +1.0 1,293,698 - Pf6N2E2_1106 0.53 +1.4 1,293,913 + Pf6N2E2_1106 0.76 +0.7 1,293,914 - Pf6N2E2_1106 0.76 +0.0 1,293,914 - Pf6N2E2_1106 0.76 -0.7 1,294,028 + Pf6N2E2_1106 0.88 +0.7 1,294,028 + Pf6N2E2_1106 0.88 -1.0 1,294,029 - Pf6N2E2_1106 0.89 -0.7 1,294,029 - Pf6N2E2_1106 0.89 -1.4 1,294,199 - -0.3 1,294,199 - +0.7 1,294,372 + -1.3 1,294,372 + +0.6 1,294,372 + +0.9 1,294,373 - -1.1 1,294,373 - +0.3 1,294,373 - +0.3 1,294,373 - +0.4 1,294,373 - -1.7 1,294,373 - +0.3 1,294,434 - -0.7 1,294,584 + -1.7 1,294,585 - -1.3 1,294,585 - +0.5 1,294,824 + Pf6N2E2_1107 0.11 +0.1 1,294,824 + Pf6N2E2_1107 0.11 +2.2 1,294,825 - Pf6N2E2_1107 0.11 -0.0 1,294,825 - Pf6N2E2_1107 0.11 -0.1 1,294,825 - Pf6N2E2_1107 0.11 -0.3 1,294,833 + Pf6N2E2_1107 0.11 +1.8 1,294,833 + Pf6N2E2_1107 0.11 -0.7 1,294,834 - Pf6N2E2_1107 0.11 -2.7 1,294,834 - Pf6N2E2_1107 0.11 -0.7 1,294,917 + Pf6N2E2_1107 0.13 +0.7 1,294,918 - Pf6N2E2_1107 0.13 -0.7 1,295,047 + Pf6N2E2_1107 0.15 +0.7 1,295,242 + Pf6N2E2_1107 0.19 +0.8 1,295,242 + Pf6N2E2_1107 0.19 -0.7 1,295,423 + Pf6N2E2_1107 0.23 -0.7 1,295,607 + Pf6N2E2_1107 0.26 +0.2 1,295,608 - Pf6N2E2_1107 0.26 +0.3 1,295,624 + Pf6N2E2_1107 0.27 +0.2 1,295,625 - Pf6N2E2_1107 0.27 +0.3 1,295,669 + Pf6N2E2_1107 0.28 -0.7 1,295,669 + Pf6N2E2_1107 0.28 -0.3 1,295,669 + Pf6N2E2_1107 0.28 +0.3 1,295,886 + Pf6N2E2_1107 0.32 -0.2 1,296,526 - Pf6N2E2_1107 0.44 -2.3 1,296,838 + Pf6N2E2_1107 0.51 +0.9 1,296,838 + Pf6N2E2_1107 0.51 -0.5 1,297,180 - Pf6N2E2_1107 0.57 +1.1 1,297,447 - Pf6N2E2_1107 0.62 +0.3 1,297,447 - Pf6N2E2_1107 0.62 +1.4 1,297,683 - Pf6N2E2_1107 0.67 -3.7 1,298,009 - Pf6N2E2_1107 0.73 +2.4 1,298,009 - Pf6N2E2_1107 0.73 +0.3 1,298,095 + Pf6N2E2_1107 0.75 +1.1 1,298,322 - Pf6N2E2_1107 0.80 +0.1 1,298,322 - Pf6N2E2_1107 0.80 +1.2 1,298,345 + Pf6N2E2_1107 0.80 -1.7 1,298,345 + Pf6N2E2_1107 0.80 +0.2 1,298,346 - Pf6N2E2_1107 0.80 +0.7 1,298,407 - Pf6N2E2_1107 0.81 +1.1 1,298,589 + Pf6N2E2_1107 0.85 +0.3 1,298,589 + Pf6N2E2_1107 0.85 +0.9 1,298,590 - Pf6N2E2_1107 0.85 +0.9 1,298,590 - Pf6N2E2_1107 0.85 -0.2 1,298,592 + Pf6N2E2_1107 0.85 -3.6 1,298,592 + Pf6N2E2_1107 0.85 -2.0 1,298,592 + Pf6N2E2_1107 0.85 -1.3 1,298,593 - Pf6N2E2_1107 0.85 -0.4 1,298,613 + Pf6N2E2_1107 0.85 +0.7 1,298,613 + Pf6N2E2_1107 0.85 +1.3 1,298,613 + Pf6N2E2_1107 0.85 +1.1 1,298,614 - Pf6N2E2_1107 0.85 +0.6 1,298,614 - Pf6N2E2_1107 0.85 +0.1 1,298,614 - Pf6N2E2_1107 0.85 +0.7 1,298,614 - Pf6N2E2_1107 0.85 +0.4 1,298,614 - Pf6N2E2_1107 0.85 +0.9 1,298,614 - Pf6N2E2_1107 0.85 +1.2 1,298,614 - Pf6N2E2_1107 0.85 -0.3 1,298,818 - Pf6N2E2_1107 0.89 -0.7 1,298,831 - Pf6N2E2_1107 0.90 -0.7 1,299,088 + -2.5 1,299,267 + -0.3 1,299,267 + +1.1 1,299,267 + +0.3 1,299,267 + +0.5 1,299,267 + +0.1 1,299,267 + +0.7 1,299,268 - -2.5 1,299,268 - -1.9 1,299,268 - -1.5 1,299,268 - -0.3 1,299,268 - +0.3 1,299,357 + -2.3 1,299,667 + Pf6N2E2_1108 0.29 +0.3 1,300,049 - Pf6N2E2_1108 0.65 +1.2 1,300,059 + Pf6N2E2_1108 0.66 +1.0 1,300,070 + Pf6N2E2_1108 0.67 +0.7 1,300,103 + Pf6N2E2_1108 0.70 +1.9 1,300,103 + Pf6N2E2_1108 0.70 +1.7 1,300,103 + Pf6N2E2_1108 0.70 +1.2 1,300,104 - Pf6N2E2_1108 0.70 -1.3 1,300,359 + +0.3 1,300,359 + +0.2
Or see this region's nucleotide sequence