Experiment: RCH2 defined with Nickel (II) chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_481 and Pf6N2E2_482 are separated by 192 nucleotides Pf6N2E2_482 and Pf6N2E2_483 are separated by 151 nucleotides Pf6N2E2_483 and Pf6N2E2_484 are separated by 320 nucleotides
Pf6N2E2_481 - branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit (EC 1.2.4.4) <small>(from data)</small>, at 547,221 to 548,456
_481
Pf6N2E2_482 - Transcriptional regulator BkdR of isoleucine and valine catabolism operon, at 548,649 to 549,137
_482
Pf6N2E2_483 - FIG146518: Zn-dependent hydrolases, including glyoxylases, at 549,289 to 550,152
_483
Pf6N2E2_484 - Aldose 1-epimerase, at 550,473 to 551,342
_484
Position (kb) 549
550
551 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 548.345 kb on + strand at 548.377 kb on + strand at 548.378 kb on - strand at 548.464 kb on + strand at 548.465 kb on - strand at 548.465 kb on - strand at 548.465 kb on - strand at 548.489 kb on + strand at 548.502 kb on - strand at 548.537 kb on + strand at 548.538 kb on - strand at 548.548 kb on + strand at 548.549 kb on - strand at 548.549 kb on - strand at 548.829 kb on - strand, within Pf6N2E2_482 at 548.943 kb on + strand, within Pf6N2E2_482 at 548.943 kb on + strand, within Pf6N2E2_482 at 549.290 kb on + strand at 549.291 kb on - strand at 549.291 kb on - strand at 549.291 kb on - strand at 549.317 kb on + strand at 549.317 kb on + strand at 549.318 kb on - strand at 549.533 kb on + strand, within Pf6N2E2_483 at 549.548 kb on + strand, within Pf6N2E2_483 at 549.548 kb on + strand, within Pf6N2E2_483 at 549.548 kb on + strand, within Pf6N2E2_483 at 549.548 kb on + strand, within Pf6N2E2_483 at 549.549 kb on - strand, within Pf6N2E2_483 at 549.549 kb on - strand, within Pf6N2E2_483 at 549.549 kb on - strand, within Pf6N2E2_483 at 549.596 kb on + strand, within Pf6N2E2_483 at 549.597 kb on - strand, within Pf6N2E2_483 at 549.597 kb on - strand, within Pf6N2E2_483 at 549.698 kb on + strand, within Pf6N2E2_483 at 549.911 kb on + strand, within Pf6N2E2_483 at 549.912 kb on - strand at 549.912 kb on - strand, within Pf6N2E2_483 at 550.100 kb on + strand at 550.100 kb on + strand at 550.100 kb on + strand at 550.100 kb on + strand at 550.100 kb on + strand at 550.101 kb on - strand at 550.101 kb on - strand at 550.101 kb on - strand at 550.106 kb on + strand at 550.106 kb on + strand at 550.106 kb on + strand at 550.106 kb on + strand at 550.106 kb on + strand at 550.107 kb on - strand at 550.107 kb on - strand at 550.107 kb on - strand at 550.107 kb on - strand at 550.237 kb on + strand at 550.243 kb on - strand at 550.266 kb on + strand at 550.267 kb on - strand at 550.267 kb on - strand at 550.267 kb on - strand at 550.267 kb on - strand at 550.288 kb on - strand at 550.288 kb on - strand at 550.474 kb on + strand at 550.621 kb on + strand, within Pf6N2E2_484 at 550.622 kb on - strand, within Pf6N2E2_484 at 550.633 kb on + strand, within Pf6N2E2_484 at 550.633 kb on + strand, within Pf6N2E2_484 at 550.634 kb on - strand, within Pf6N2E2_484 at 550.659 kb on + strand, within Pf6N2E2_484 at 550.659 kb on + strand, within Pf6N2E2_484 at 550.659 kb on + strand, within Pf6N2E2_484 at 550.659 kb on + strand, within Pf6N2E2_484 at 550.660 kb on - strand, within Pf6N2E2_484 at 550.660 kb on - strand, within Pf6N2E2_484 at 550.660 kb on - strand, within Pf6N2E2_484 at 550.671 kb on + strand, within Pf6N2E2_484 at 550.671 kb on + strand, within Pf6N2E2_484 at 550.671 kb on + strand, within Pf6N2E2_484 at 550.672 kb on - strand, within Pf6N2E2_484 at 550.672 kb on - strand, within Pf6N2E2_484 at 550.672 kb on - strand, within Pf6N2E2_484 at 550.672 kb on - strand, within Pf6N2E2_484 at 550.672 kb on - strand, within Pf6N2E2_484 at 550.672 kb on - strand, within Pf6N2E2_484 at 550.672 kb on - strand, within Pf6N2E2_484 at 550.672 kb on - strand, within Pf6N2E2_484 at 550.743 kb on + strand, within Pf6N2E2_484 at 550.870 kb on + strand, within Pf6N2E2_484 at 550.871 kb on - strand, within Pf6N2E2_484
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Nickel (II) chloride 0.04 mM remove 548,345 + +1.2 548,377 + -1.0 548,378 - +0.2 548,464 + -0.3 548,465 - +0.7 548,465 - -0.1 548,465 - -0.0 548,489 + +0.6 548,502 - -0.1 548,537 + +0.7 548,538 - +0.0 548,548 + +0.5 548,549 - -0.9 548,549 - +1.7 548,829 - Pf6N2E2_482 0.37 -1.9 548,943 + Pf6N2E2_482 0.60 +1.2 548,943 + Pf6N2E2_482 0.60 +0.9 549,290 + +0.2 549,291 - +0.5 549,291 - +0.4 549,291 - -1.6 549,317 + +0.1 549,317 + +0.0 549,318 - +0.3 549,533 + Pf6N2E2_483 0.28 -1.5 549,548 + Pf6N2E2_483 0.30 -0.9 549,548 + Pf6N2E2_483 0.30 +2.0 549,548 + Pf6N2E2_483 0.30 -1.6 549,548 + Pf6N2E2_483 0.30 -2.6 549,549 - Pf6N2E2_483 0.30 +0.8 549,549 - Pf6N2E2_483 0.30 -1.3 549,549 - Pf6N2E2_483 0.30 -0.9 549,596 + Pf6N2E2_483 0.36 -0.1 549,597 - Pf6N2E2_483 0.36 +0.6 549,597 - Pf6N2E2_483 0.36 +0.2 549,698 + Pf6N2E2_483 0.47 -0.9 549,911 + Pf6N2E2_483 0.72 -2.0 549,912 - +0.7 549,912 - Pf6N2E2_483 0.72 -0.9 550,100 + +1.5 550,100 + +1.6 550,100 + -0.2 550,100 + +0.7 550,100 + -0.4 550,101 - +1.1 550,101 - +0.9 550,101 - -0.3 550,106 + +0.6 550,106 + +0.5 550,106 + +2.3 550,106 + -0.4 550,106 + -1.2 550,107 - +0.3 550,107 - +0.7 550,107 - +1.8 550,107 - +0.1 550,237 + -2.1 550,243 - -0.9 550,266 + -2.3 550,267 - -0.9 550,267 - +0.1 550,267 - -0.3 550,267 - +1.0 550,288 - -0.3 550,288 - +1.2 550,474 + -2.1 550,621 + Pf6N2E2_484 0.17 -1.3 550,622 - Pf6N2E2_484 0.17 -2.5 550,633 + Pf6N2E2_484 0.18 -0.6 550,633 + Pf6N2E2_484 0.18 -0.7 550,634 - Pf6N2E2_484 0.19 -0.1 550,659 + Pf6N2E2_484 0.21 -0.1 550,659 + Pf6N2E2_484 0.21 -0.9 550,659 + Pf6N2E2_484 0.21 +0.1 550,659 + Pf6N2E2_484 0.21 -2.2 550,660 - Pf6N2E2_484 0.21 -1.2 550,660 - Pf6N2E2_484 0.21 +0.3 550,660 - Pf6N2E2_484 0.21 +0.7 550,671 + Pf6N2E2_484 0.23 -0.4 550,671 + Pf6N2E2_484 0.23 +0.7 550,671 + Pf6N2E2_484 0.23 -1.3 550,672 - Pf6N2E2_484 0.23 +0.5 550,672 - Pf6N2E2_484 0.23 +0.7 550,672 - Pf6N2E2_484 0.23 -0.8 550,672 - Pf6N2E2_484 0.23 -0.4 550,672 - Pf6N2E2_484 0.23 +0.7 550,672 - Pf6N2E2_484 0.23 -0.1 550,672 - Pf6N2E2_484 0.23 -3.7 550,672 - Pf6N2E2_484 0.23 -0.3 550,743 + Pf6N2E2_484 0.31 +0.7 550,870 + Pf6N2E2_484 0.46 -0.3 550,871 - Pf6N2E2_484 0.46 +1.5
Or see this region's nucleotide sequence