Experiment: RCH2 defined with Nickel (II) chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2350 and Pf6N2E2_2351 overlap by 29 nucleotides Pf6N2E2_2351 and Pf6N2E2_2352 are separated by 4 nucleotides Pf6N2E2_2352 and Pf6N2E2_2353 overlap by 4 nucleotides Pf6N2E2_2353 and Pf6N2E2_2354 are separated by 59 nucleotides Pf6N2E2_2354 and Pf6N2E2_2355 are separated by 3 nucleotides
Pf6N2E2_2350 - Oxygen-regulated invasion protein OrgB, at 2,785,994 to 2,786,704
_2350
Pf6N2E2_2351 - Oxygen-regulated invasion protein OrgA, at 2,786,676 to 2,787,224
_2351
Pf6N2E2_2352 - Type III secretion bridge between inner and outermembrane lipoprotein (YscJ,HrcJ,EscJ, PscJ), at 2,787,229 to 2,787,948
_2352
Pf6N2E2_2353 - cell invasion protein - cytoplasmic, at 2,787,945 to 2,788,253
_2353
Pf6N2E2_2354 - MxiH protein; Type III secretion cytoplasmic protein (YscF), at 2,788,313 to 2,788,576
_2354
Pf6N2E2_2355 - MxiG protein; Pathogenicity 1 island effector protein, at 2,788,580 to 2,789,719
_2355
Position (kb)
2787
2788 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2786.920 kb on - strand, within Pf6N2E2_2351 at 2787.023 kb on + strand, within Pf6N2E2_2351 at 2787.023 kb on + strand, within Pf6N2E2_2351 at 2787.024 kb on - strand, within Pf6N2E2_2351 at 2787.024 kb on - strand, within Pf6N2E2_2351 at 2787.026 kb on - strand, within Pf6N2E2_2351 at 2787.026 kb on - strand, within Pf6N2E2_2351 at 2787.026 kb on - strand, within Pf6N2E2_2351 at 2787.132 kb on + strand, within Pf6N2E2_2351 at 2787.133 kb on - strand, within Pf6N2E2_2351 at 2787.133 kb on - strand, within Pf6N2E2_2351 at 2787.193 kb on + strand at 2787.194 kb on - strand at 2787.269 kb on + strand at 2787.413 kb on + strand, within Pf6N2E2_2352 at 2787.557 kb on + strand, within Pf6N2E2_2352 at 2787.558 kb on - strand, within Pf6N2E2_2352 at 2787.635 kb on + strand, within Pf6N2E2_2352 at 2787.636 kb on - strand, within Pf6N2E2_2352 at 2788.024 kb on + strand, within Pf6N2E2_2353 at 2788.024 kb on + strand, within Pf6N2E2_2353 at 2788.024 kb on + strand, within Pf6N2E2_2353 at 2788.025 kb on - strand, within Pf6N2E2_2353 at 2788.025 kb on - strand, within Pf6N2E2_2353 at 2788.056 kb on + strand, within Pf6N2E2_2353 at 2788.056 kb on + strand, within Pf6N2E2_2353 at 2788.056 kb on + strand, within Pf6N2E2_2353 at 2788.056 kb on + strand, within Pf6N2E2_2353 at 2788.057 kb on - strand, within Pf6N2E2_2353 at 2788.057 kb on - strand, within Pf6N2E2_2353 at 2788.253 kb on + strand at 2788.253 kb on + strand at 2788.253 kb on + strand at 2788.253 kb on + strand at 2788.253 kb on + strand at 2788.253 kb on + strand at 2788.253 kb on + strand at 2788.254 kb on - strand at 2788.254 kb on - strand at 2788.254 kb on - strand at 2788.388 kb on + strand, within Pf6N2E2_2354 at 2788.388 kb on + strand, within Pf6N2E2_2354 at 2788.388 kb on + strand, within Pf6N2E2_2354 at 2788.388 kb on + strand, within Pf6N2E2_2354 at 2788.395 kb on + strand, within Pf6N2E2_2354 at 2788.395 kb on + strand, within Pf6N2E2_2354 at 2788.396 kb on - strand, within Pf6N2E2_2354 at 2788.417 kb on - strand, within Pf6N2E2_2354 at 2788.568 kb on - strand at 2788.576 kb on + strand at 2788.576 kb on + strand at 2788.576 kb on + strand at 2788.576 kb on + strand at 2788.576 kb on + strand at 2788.576 kb on + strand at 2788.576 kb on + strand at 2788.577 kb on - strand at 2788.577 kb on - strand at 2788.577 kb on - strand at 2788.577 kb on - strand at 2788.577 kb on - strand at 2788.577 kb on - strand at 2788.577 kb on - strand at 2788.577 kb on - strand at 2788.586 kb on - strand at 2788.586 kb on - strand at 2788.586 kb on - strand at 2788.632 kb on + strand at 2788.632 kb on + strand at 2788.633 kb on - strand at 2788.633 kb on - strand at 2788.704 kb on + strand, within Pf6N2E2_2355 at 2788.704 kb on + strand, within Pf6N2E2_2355 at 2788.705 kb on - strand, within Pf6N2E2_2355 at 2788.740 kb on + strand, within Pf6N2E2_2355 at 2788.741 kb on - strand, within Pf6N2E2_2355 at 2788.741 kb on - strand, within Pf6N2E2_2355
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Nickel (II) chloride 0.04 mM remove 2,786,920 - Pf6N2E2_2351 0.44 +0.0 2,787,023 + Pf6N2E2_2351 0.63 +1.1 2,787,023 + Pf6N2E2_2351 0.63 -0.3 2,787,024 - Pf6N2E2_2351 0.63 -1.8 2,787,024 - Pf6N2E2_2351 0.63 -0.3 2,787,026 - Pf6N2E2_2351 0.64 +0.7 2,787,026 - Pf6N2E2_2351 0.64 +0.5 2,787,026 - Pf6N2E2_2351 0.64 +1.4 2,787,132 + Pf6N2E2_2351 0.83 +0.7 2,787,133 - Pf6N2E2_2351 0.83 +0.8 2,787,133 - Pf6N2E2_2351 0.83 +0.4 2,787,193 + +0.3 2,787,194 - -0.4 2,787,269 + +0.7 2,787,413 + Pf6N2E2_2352 0.26 -1.3 2,787,557 + Pf6N2E2_2352 0.46 +0.4 2,787,558 - Pf6N2E2_2352 0.46 -1.9 2,787,635 + Pf6N2E2_2352 0.56 -0.7 2,787,636 - Pf6N2E2_2352 0.57 -1.5 2,788,024 + Pf6N2E2_2353 0.26 +0.3 2,788,024 + Pf6N2E2_2353 0.26 -0.2 2,788,024 + Pf6N2E2_2353 0.26 +0.1 2,788,025 - Pf6N2E2_2353 0.26 +0.7 2,788,025 - Pf6N2E2_2353 0.26 +0.8 2,788,056 + Pf6N2E2_2353 0.36 +0.1 2,788,056 + Pf6N2E2_2353 0.36 -0.2 2,788,056 + Pf6N2E2_2353 0.36 +0.7 2,788,056 + Pf6N2E2_2353 0.36 -0.3 2,788,057 - Pf6N2E2_2353 0.36 -1.3 2,788,057 - Pf6N2E2_2353 0.36 -2.5 2,788,253 + +0.4 2,788,253 + -1.3 2,788,253 + -1.3 2,788,253 + -0.2 2,788,253 + +0.2 2,788,253 + -0.3 2,788,253 + -1.6 2,788,254 - -0.2 2,788,254 - +1.1 2,788,254 - +0.1 2,788,388 + Pf6N2E2_2354 0.28 +1.1 2,788,388 + Pf6N2E2_2354 0.28 -0.3 2,788,388 + Pf6N2E2_2354 0.28 +1.0 2,788,388 + Pf6N2E2_2354 0.28 -0.4 2,788,395 + Pf6N2E2_2354 0.31 -0.6 2,788,395 + Pf6N2E2_2354 0.31 -0.5 2,788,396 - Pf6N2E2_2354 0.31 -0.7 2,788,417 - Pf6N2E2_2354 0.39 -2.1 2,788,568 - +0.7 2,788,576 + -0.5 2,788,576 + -1.3 2,788,576 + -0.5 2,788,576 + -0.9 2,788,576 + -2.3 2,788,576 + +0.3 2,788,576 + +0.2 2,788,577 - +1.1 2,788,577 - +1.2 2,788,577 - +2.0 2,788,577 - -1.3 2,788,577 - +1.5 2,788,577 - +0.0 2,788,577 - -0.2 2,788,577 - +2.7 2,788,586 - -0.1 2,788,586 - +0.2 2,788,586 - +0.1 2,788,632 + +0.7 2,788,632 + +0.0 2,788,633 - -0.0 2,788,633 - -0.1 2,788,704 + Pf6N2E2_2355 0.11 -0.3 2,788,704 + Pf6N2E2_2355 0.11 +0.9 2,788,705 - Pf6N2E2_2355 0.11 +0.7 2,788,740 + Pf6N2E2_2355 0.14 +1.2 2,788,741 - Pf6N2E2_2355 0.14 -0.0 2,788,741 - Pf6N2E2_2355 0.14 -0.3
Or see this region's nucleotide sequence