Experiment: Ammonium chloride (N); Varel_Bryant_medium_Glucose_lowCys_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS16700 and HMPREF1058_RS16695 are separated by 102 nucleotides HMPREF1058_RS16695 and HMPREF1058_RS16690 overlap by 4 nucleotides
HMPREF1058_RS16700: HMPREF1058_RS16700 - purine permease, at 613,206 to 614,537
_RS16700
HMPREF1058_RS16695: HMPREF1058_RS16695 - phosphotransferase, at 614,640 to 616,070
_RS16695
HMPREF1058_RS16690: HMPREF1058_RS16690 - NTP transferase domain-containing protein, at 616,067 to 616,912
_RS16690
Position (kb)
614
615
616
617 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 613.673 kb on - strand, within HMPREF1058_RS16700 at 613.682 kb on + strand, within HMPREF1058_RS16700 at 613.762 kb on + strand, within HMPREF1058_RS16700 at 613.762 kb on + strand, within HMPREF1058_RS16700 at 613.853 kb on - strand, within HMPREF1058_RS16700 at 613.853 kb on - strand, within HMPREF1058_RS16700 at 613.891 kb on + strand, within HMPREF1058_RS16700 at 613.928 kb on + strand, within HMPREF1058_RS16700 at 613.928 kb on + strand, within HMPREF1058_RS16700 at 613.963 kb on + strand, within HMPREF1058_RS16700 at 613.978 kb on + strand, within HMPREF1058_RS16700 at 613.996 kb on + strand, within HMPREF1058_RS16700 at 613.997 kb on - strand, within HMPREF1058_RS16700 at 614.170 kb on + strand, within HMPREF1058_RS16700 at 614.170 kb on + strand, within HMPREF1058_RS16700 at 614.186 kb on - strand, within HMPREF1058_RS16700 at 614.200 kb on + strand, within HMPREF1058_RS16700 at 614.200 kb on + strand, within HMPREF1058_RS16700 at 614.201 kb on - strand, within HMPREF1058_RS16700 at 614.252 kb on - strand, within HMPREF1058_RS16700 at 614.252 kb on - strand, within HMPREF1058_RS16700 at 614.321 kb on - strand, within HMPREF1058_RS16700 at 614.377 kb on + strand, within HMPREF1058_RS16700 at 614.391 kb on - strand, within HMPREF1058_RS16700 at 614.408 kb on - strand at 614.413 kb on + strand at 614.435 kb on - strand at 614.480 kb on + strand at 614.556 kb on + strand at 614.567 kb on + strand at 614.613 kb on + strand at 614.613 kb on + strand at 614.614 kb on - strand at 614.615 kb on + strand at 614.616 kb on - strand at 614.617 kb on + strand at 614.617 kb on + strand at 614.640 kb on - strand at 614.665 kb on + strand at 614.666 kb on - strand at 614.666 kb on - strand at 614.666 kb on - strand at 614.676 kb on + strand at 614.704 kb on + strand at 614.857 kb on + strand, within HMPREF1058_RS16695 at 614.859 kb on + strand, within HMPREF1058_RS16695 at 614.859 kb on + strand, within HMPREF1058_RS16695 at 614.859 kb on + strand, within HMPREF1058_RS16695 at 614.860 kb on - strand, within HMPREF1058_RS16695 at 614.887 kb on - strand, within HMPREF1058_RS16695 at 614.887 kb on - strand, within HMPREF1058_RS16695 at 615.184 kb on + strand, within HMPREF1058_RS16695 at 615.290 kb on + strand, within HMPREF1058_RS16695 at 615.290 kb on + strand, within HMPREF1058_RS16695 at 615.313 kb on + strand, within HMPREF1058_RS16695 at 615.342 kb on - strand, within HMPREF1058_RS16695 at 615.381 kb on + strand, within HMPREF1058_RS16695 at 615.382 kb on - strand, within HMPREF1058_RS16695 at 615.382 kb on - strand, within HMPREF1058_RS16695 at 615.412 kb on + strand, within HMPREF1058_RS16695 at 615.423 kb on + strand, within HMPREF1058_RS16695 at 615.499 kb on - strand, within HMPREF1058_RS16695 at 615.591 kb on + strand, within HMPREF1058_RS16695 at 615.607 kb on + strand, within HMPREF1058_RS16695 at 615.732 kb on + strand, within HMPREF1058_RS16695 at 615.733 kb on - strand, within HMPREF1058_RS16695 at 615.733 kb on - strand, within HMPREF1058_RS16695 at 615.841 kb on + strand, within HMPREF1058_RS16695 at 615.989 kb on + strand at 616.024 kb on + strand at 616.042 kb on + strand at 616.042 kb on + strand at 616.043 kb on - strand at 616.043 kb on - strand at 616.109 kb on - strand at 616.212 kb on + strand, within HMPREF1058_RS16690 at 616.212 kb on + strand, within HMPREF1058_RS16690 at 616.354 kb on - strand, within HMPREF1058_RS16690 at 616.372 kb on + strand, within HMPREF1058_RS16690 at 616.373 kb on - strand, within HMPREF1058_RS16690 at 616.487 kb on + strand, within HMPREF1058_RS16690 at 616.487 kb on + strand, within HMPREF1058_RS16690 at 616.503 kb on + strand, within HMPREF1058_RS16690 at 616.504 kb on - strand, within HMPREF1058_RS16690 at 616.504 kb on - strand, within HMPREF1058_RS16690 at 616.544 kb on + strand, within HMPREF1058_RS16690 at 616.556 kb on - strand, within HMPREF1058_RS16690 at 616.619 kb on - strand, within HMPREF1058_RS16690 at 616.635 kb on + strand, within HMPREF1058_RS16690 at 616.690 kb on + strand, within HMPREF1058_RS16690 at 616.712 kb on + strand, within HMPREF1058_RS16690 at 616.712 kb on + strand, within HMPREF1058_RS16690 at 616.776 kb on - strand, within HMPREF1058_RS16690 at 616.829 kb on + strand at 616.829 kb on + strand at 616.830 kb on - strand at 616.928 kb on + strand at 616.928 kb on + strand at 616.962 kb on - strand at 617.057 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N); Varel_Bryant_medium_Glucose_lowCys_noNitrogen remove 613,673 - HMPREF1058_RS16700 0.35 +0.3 613,682 + HMPREF1058_RS16700 0.36 -0.6 613,762 + HMPREF1058_RS16700 0.42 +0.6 613,762 + HMPREF1058_RS16700 0.42 -0.1 613,853 - HMPREF1058_RS16700 0.49 -1.0 613,853 - HMPREF1058_RS16700 0.49 -0.6 613,891 + HMPREF1058_RS16700 0.51 +0.4 613,928 + HMPREF1058_RS16700 0.54 +1.3 613,928 + HMPREF1058_RS16700 0.54 +0.7 613,963 + HMPREF1058_RS16700 0.57 -0.8 613,978 + HMPREF1058_RS16700 0.58 +0.1 613,996 + HMPREF1058_RS16700 0.59 +0.7 613,997 - HMPREF1058_RS16700 0.59 -0.3 614,170 + HMPREF1058_RS16700 0.72 -0.3 614,170 + HMPREF1058_RS16700 0.72 +1.1 614,186 - HMPREF1058_RS16700 0.74 +1.3 614,200 + HMPREF1058_RS16700 0.75 +0.2 614,200 + HMPREF1058_RS16700 0.75 +0.4 614,201 - HMPREF1058_RS16700 0.75 +0.0 614,252 - HMPREF1058_RS16700 0.79 -0.2 614,252 - HMPREF1058_RS16700 0.79 +1.0 614,321 - HMPREF1058_RS16700 0.84 +0.6 614,377 + HMPREF1058_RS16700 0.88 +0.5 614,391 - HMPREF1058_RS16700 0.89 +2.4 614,408 - +0.4 614,413 + +0.3 614,435 - -1.4 614,480 + +0.6 614,556 + +0.2 614,567 + +0.8 614,613 + +1.4 614,613 + +0.2 614,614 - -0.2 614,615 + -0.4 614,616 - -0.3 614,617 + -1.5 614,617 + -0.7 614,640 - -1.0 614,665 + -0.8 614,666 - -1.3 614,666 - +0.1 614,666 - -1.3 614,676 + +0.2 614,704 + -0.4 614,857 + HMPREF1058_RS16695 0.15 -0.4 614,859 + HMPREF1058_RS16695 0.15 -1.6 614,859 + HMPREF1058_RS16695 0.15 -1.6 614,859 + HMPREF1058_RS16695 0.15 +0.1 614,860 - HMPREF1058_RS16695 0.15 -0.2 614,887 - HMPREF1058_RS16695 0.17 -0.8 614,887 - HMPREF1058_RS16695 0.17 -0.6 615,184 + HMPREF1058_RS16695 0.38 +1.1 615,290 + HMPREF1058_RS16695 0.45 -2.5 615,290 + HMPREF1058_RS16695 0.45 -1.0 615,313 + HMPREF1058_RS16695 0.47 -0.9 615,342 - HMPREF1058_RS16695 0.49 -1.2 615,381 + HMPREF1058_RS16695 0.52 -1.4 615,382 - HMPREF1058_RS16695 0.52 -1.6 615,382 - HMPREF1058_RS16695 0.52 -0.2 615,412 + HMPREF1058_RS16695 0.54 -0.6 615,423 + HMPREF1058_RS16695 0.55 -0.7 615,499 - HMPREF1058_RS16695 0.60 -0.9 615,591 + HMPREF1058_RS16695 0.66 -0.2 615,607 + HMPREF1058_RS16695 0.68 -0.1 615,732 + HMPREF1058_RS16695 0.76 -3.3 615,733 - HMPREF1058_RS16695 0.76 +0.2 615,733 - HMPREF1058_RS16695 0.76 -1.8 615,841 + HMPREF1058_RS16695 0.84 -3.9 615,989 + +0.6 616,024 + -0.6 616,042 + +0.9 616,042 + -0.9 616,043 - +1.5 616,043 - -1.0 616,109 - -3.2 616,212 + HMPREF1058_RS16690 0.17 -2.5 616,212 + HMPREF1058_RS16690 0.17 -1.9 616,354 - HMPREF1058_RS16690 0.34 -3.4 616,372 + HMPREF1058_RS16690 0.36 -3.2 616,373 - HMPREF1058_RS16690 0.36 -2.4 616,487 + HMPREF1058_RS16690 0.50 -1.2 616,487 + HMPREF1058_RS16690 0.50 -2.0 616,503 + HMPREF1058_RS16690 0.52 -0.3 616,504 - HMPREF1058_RS16690 0.52 -2.6 616,504 - HMPREF1058_RS16690 0.52 -2.7 616,544 + HMPREF1058_RS16690 0.56 -1.1 616,556 - HMPREF1058_RS16690 0.58 -2.1 616,619 - HMPREF1058_RS16690 0.65 -1.9 616,635 + HMPREF1058_RS16690 0.67 -1.7 616,690 + HMPREF1058_RS16690 0.74 -0.8 616,712 + HMPREF1058_RS16690 0.76 -2.1 616,712 + HMPREF1058_RS16690 0.76 -2.7 616,776 - HMPREF1058_RS16690 0.84 -2.5 616,829 + -2.3 616,829 + -2.7 616,830 - -0.2 616,928 + -1.1 616,928 + +2.0 616,962 - -0.5 617,057 - +0.4
Or see this region's nucleotide sequence