Experiment: Arabinoxylan (Rye Flour); autoclaved 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS02000 and HMPREF1058_RS01995 are separated by 56 nucleotides HMPREF1058_RS01995 and HMPREF1058_RS01990 are separated by 183 nucleotides HMPREF1058_RS01990 and HMPREF1058_RS01985 overlap by 35 nucleotides HMPREF1058_RS01985 and HMPREF1058_RS01980 are separated by 16 nucleotides HMPREF1058_RS01980 and HMPREF1058_RS01975 overlap by 29 nucleotides HMPREF1058_RS01975 and HMPREF1058_RS01970 are separated by 148 nucleotides HMPREF1058_RS01970 and HMPREF1058_RS21540 are separated by 42 nucleotides
HMPREF1058_RS02000: HMPREF1058_RS02000 - type II toxin-antitoxin system HicA family toxin, at 2,530 to 2,715
_RS02000
HMPREF1058_RS01995: HMPREF1058_RS01995 - hypothetical protein, at 2,772 to 3,176
_RS01995
HMPREF1058_RS01990: HMPREF1058_RS01990 - hypothetical protein, at 3,360 to 3,746
_RS01990
HMPREF1058_RS01985: HMPREF1058_RS01985 - transposase, at 3,712 to 4,704
_RS01985
HMPREF1058_RS01980: HMPREF1058_RS01980 - hypothetical protein, at 4,721 to 5,095
_RS01980
HMPREF1058_RS01975: HMPREF1058_RS01975 - hypothetical protein, at 5,067 to 5,333
_RS01975
HMPREF1058_RS01970: HMPREF1058_RS01970 - hypothetical protein, at 5,482 to 5,661
_RS01970
HMPREF1058_RS21540: HMPREF1058_RS21540 - hypothetical protein, at 5,704 to 5,856
_RS21540
Position (kb) 3
4
5 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 2.957 kb on + strand, within HMPREF1058_RS01995 at 2.979 kb on + strand, within HMPREF1058_RS01995 at 2.983 kb on + strand, within HMPREF1058_RS01995 at 2.985 kb on + strand, within HMPREF1058_RS01995 at 3.078 kb on + strand, within HMPREF1058_RS01995 at 3.135 kb on + strand, within HMPREF1058_RS01995 at 3.253 kb on - strand at 3.281 kb on - strand at 4.687 kb on + strand at 4.736 kb on - strand at 4.750 kb on + strand at 4.759 kb on + strand, within HMPREF1058_RS01980 at 4.772 kb on + strand, within HMPREF1058_RS01980 at 4.773 kb on - strand, within HMPREF1058_RS01980 at 4.774 kb on + strand, within HMPREF1058_RS01980 at 4.817 kb on - strand, within HMPREF1058_RS01980 at 4.817 kb on - strand, within HMPREF1058_RS01980 at 4.817 kb on - strand, within HMPREF1058_RS01980 at 4.822 kb on - strand, within HMPREF1058_RS01980 at 4.829 kb on - strand, within HMPREF1058_RS01980 at 4.829 kb on - strand, within HMPREF1058_RS01980 at 4.894 kb on + strand, within HMPREF1058_RS01980 at 4.927 kb on + strand, within HMPREF1058_RS01980 at 4.927 kb on + strand, within HMPREF1058_RS01980 at 4.959 kb on + strand, within HMPREF1058_RS01980 at 4.960 kb on - strand, within HMPREF1058_RS01980 at 4.988 kb on - strand, within HMPREF1058_RS01980 at 5.000 kb on - strand, within HMPREF1058_RS01980 at 5.097 kb on + strand, within HMPREF1058_RS01975 at 5.097 kb on + strand, within HMPREF1058_RS01975 at 5.098 kb on - strand, within HMPREF1058_RS01975 at 5.246 kb on - strand, within HMPREF1058_RS01975 at 5.246 kb on - strand, within HMPREF1058_RS01975 at 5.305 kb on - strand, within HMPREF1058_RS01975 at 5.305 kb on - strand, within HMPREF1058_RS01975 at 5.349 kb on - strand at 5.459 kb on + strand at 5.460 kb on - strand at 5.476 kb on - strand at 5.528 kb on + strand, within HMPREF1058_RS01970 at 5.529 kb on - strand, within HMPREF1058_RS01970 at 5.531 kb on + strand, within HMPREF1058_RS01970 at 5.573 kb on + strand, within HMPREF1058_RS01970 at 5.573 kb on + strand, within HMPREF1058_RS01970 at 5.596 kb on + strand, within HMPREF1058_RS01970 at 5.625 kb on + strand, within HMPREF1058_RS01970 at 5.695 kb on + strand at 5.696 kb on - strand at 5.696 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Arabinoxylan (Rye Flour); autoclaved 2.5 mg/ml (C) remove 2,957 + HMPREF1058_RS01995 0.46 -1.4 2,979 + HMPREF1058_RS01995 0.51 +2.1 2,983 + HMPREF1058_RS01995 0.52 -0.2 2,985 + HMPREF1058_RS01995 0.53 +0.5 3,078 + HMPREF1058_RS01995 0.76 -0.5 3,135 + HMPREF1058_RS01995 0.90 +2.1 3,253 - -0.5 3,281 - +0.4 4,687 + -1.4 4,736 - +1.1 4,750 + -0.7 4,759 + HMPREF1058_RS01980 0.10 -0.3 4,772 + HMPREF1058_RS01980 0.14 -0.7 4,773 - HMPREF1058_RS01980 0.14 -0.0 4,774 + HMPREF1058_RS01980 0.14 -1.7 4,817 - HMPREF1058_RS01980 0.26 -0.1 4,817 - HMPREF1058_RS01980 0.26 -2.7 4,817 - HMPREF1058_RS01980 0.26 -4.1 4,822 - HMPREF1058_RS01980 0.27 -0.8 4,829 - HMPREF1058_RS01980 0.29 -3.8 4,829 - HMPREF1058_RS01980 0.29 +0.1 4,894 + HMPREF1058_RS01980 0.46 +1.4 4,927 + HMPREF1058_RS01980 0.55 +1.2 4,927 + HMPREF1058_RS01980 0.55 -0.8 4,959 + HMPREF1058_RS01980 0.63 -1.4 4,960 - HMPREF1058_RS01980 0.64 +1.2 4,988 - HMPREF1058_RS01980 0.71 +1.1 5,000 - HMPREF1058_RS01980 0.74 +2.1 5,097 + HMPREF1058_RS01975 0.11 -0.8 5,097 + HMPREF1058_RS01975 0.11 +0.5 5,098 - HMPREF1058_RS01975 0.12 -3.3 5,246 - HMPREF1058_RS01975 0.67 +0.6 5,246 - HMPREF1058_RS01975 0.67 +1.1 5,305 - HMPREF1058_RS01975 0.89 +1.4 5,305 - HMPREF1058_RS01975 0.89 -0.2 5,349 - -0.4 5,459 + +1.4 5,460 - +0.2 5,476 - -1.5 5,528 + HMPREF1058_RS01970 0.26 +1.1 5,529 - HMPREF1058_RS01970 0.26 +0.7 5,531 + HMPREF1058_RS01970 0.27 -0.9 5,573 + HMPREF1058_RS01970 0.51 +1.1 5,573 + HMPREF1058_RS01970 0.51 -0.5 5,596 + HMPREF1058_RS01970 0.63 +1.2 5,625 + HMPREF1058_RS01970 0.79 +1.3 5,695 + +0.3 5,696 - +0.0 5,696 - +2.2
Or see this region's nucleotide sequence