Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05885 and BWI76_RS05890 are separated by 214 nucleotides BWI76_RS05890 and BWI76_RS05895 are separated by 144 nucleotides
BWI76_RS05885: BWI76_RS05885 - anion permease, at 1,237,087 to 1,237,896
_RS05885
BWI76_RS05890: BWI76_RS05890 - haloacid dehalogenase, at 1,238,111 to 1,238,773
_RS05890
BWI76_RS05895: BWI76_RS05895 - phosphonate ABC transporter, permease protein PhnE, at 1,238,918 to 1,239,799
_RS05895
Position (kb)
1238
1239 Strain fitness (log2 ratio)
-1
0
1 at 1237.174 kb on - strand, within BWI76_RS05885 at 1237.282 kb on - strand, within BWI76_RS05885 at 1237.538 kb on + strand, within BWI76_RS05885 at 1237.680 kb on + strand, within BWI76_RS05885 at 1237.883 kb on - strand at 1238.123 kb on - strand at 1238.192 kb on - strand, within BWI76_RS05890 at 1238.192 kb on - strand, within BWI76_RS05890 at 1238.192 kb on - strand, within BWI76_RS05890 at 1238.311 kb on + strand, within BWI76_RS05890 at 1238.511 kb on + strand, within BWI76_RS05890 at 1238.568 kb on + strand, within BWI76_RS05890 at 1238.592 kb on + strand, within BWI76_RS05890 at 1238.706 kb on + strand, within BWI76_RS05890 at 1238.843 kb on - strand at 1238.903 kb on - strand at 1238.925 kb on + strand at 1239.027 kb on - strand, within BWI76_RS05895 at 1239.207 kb on + strand, within BWI76_RS05895 at 1239.711 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 1,237,174 - BWI76_RS05885 0.11 +1.2 1,237,282 - BWI76_RS05885 0.24 +1.4 1,237,538 + BWI76_RS05885 0.56 +1.6 1,237,680 + BWI76_RS05885 0.73 +0.2 1,237,883 - +0.1 1,238,123 - -0.3 1,238,192 - BWI76_RS05890 0.12 +0.5 1,238,192 - BWI76_RS05890 0.12 +0.4 1,238,192 - BWI76_RS05890 0.12 -0.0 1,238,311 + BWI76_RS05890 0.30 -0.1 1,238,511 + BWI76_RS05890 0.60 +0.4 1,238,568 + BWI76_RS05890 0.69 +0.4 1,238,592 + BWI76_RS05890 0.73 +0.4 1,238,706 + BWI76_RS05890 0.90 -0.1 1,238,843 - +0.0 1,238,903 - +0.0 1,238,925 + -0.3 1,239,027 - BWI76_RS05895 0.12 +0.1 1,239,207 + BWI76_RS05895 0.33 -0.1 1,239,711 - +1.3
Or see this region's nucleotide sequence