Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05200 and BWI76_RS05205 are separated by 131 nucleotides BWI76_RS05205 and BWI76_RS05210 are separated by 323 nucleotides
BWI76_RS05200: BWI76_RS05200 - bifunctional uridylyltransferase/uridylyl-removing protein, at 1,093,788 to 1,096,451
_RS05200
BWI76_RS05205: BWI76_RS05205 - type I methionyl aminopeptidase, at 1,096,583 to 1,097,377
_RS05205
BWI76_RS05210: BWI76_RS05210 - 30S ribosomal protein S2, at 1,097,701 to 1,098,426
_RS05210
Position (kb)
1096
1097
1098 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1095.761 kb on + strand, within BWI76_RS05200 at 1095.949 kb on - strand, within BWI76_RS05200 at 1096.423 kb on - strand at 1096.437 kb on - strand at 1096.654 kb on - strand at 1096.672 kb on + strand, within BWI76_RS05205 at 1096.689 kb on - strand, within BWI76_RS05205 at 1096.786 kb on - strand, within BWI76_RS05205 at 1096.826 kb on - strand, within BWI76_RS05205 at 1096.826 kb on - strand, within BWI76_RS05205 at 1096.840 kb on - strand, within BWI76_RS05205 at 1096.988 kb on - strand, within BWI76_RS05205 at 1097.052 kb on - strand, within BWI76_RS05205 at 1097.488 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 1,095,761 + BWI76_RS05200 0.74 -2.6 1,095,949 - BWI76_RS05200 0.81 -0.6 1,096,423 - -0.6 1,096,437 - +0.1 1,096,654 - -0.7 1,096,672 + BWI76_RS05205 0.11 +2.2 1,096,689 - BWI76_RS05205 0.13 +1.4 1,096,786 - BWI76_RS05205 0.26 -0.8 1,096,826 - BWI76_RS05205 0.31 -0.2 1,096,826 - BWI76_RS05205 0.31 +1.2 1,096,840 - BWI76_RS05205 0.32 +1.0 1,096,988 - BWI76_RS05205 0.51 -0.1 1,097,052 - BWI76_RS05205 0.59 -0.6 1,097,488 + -0.1
Or see this region's nucleotide sequence