Experiment: LB 0.5x with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19995 and BWI76_RS20000 are separated by 204 nucleotides BWI76_RS20000 and BWI76_RS20005 are separated by 96 nucleotides BWI76_RS20005 and BWI76_RS20010 are separated by 269 nucleotides
BWI76_RS19995: BWI76_RS19995 - IclR family transcriptional regulator, at 4,189,179 to 4,189,961
_RS19995
BWI76_RS20000: BWI76_RS20000 - competence/damage-inducible protein A, at 4,190,166 to 4,191,362
_RS20000
BWI76_RS20005: BWI76_RS20005 - membrane protein, at 4,191,459 to 4,192,001
_RS20005
BWI76_RS20010: BWI76_RS20010 - catalase, at 4,192,271 to 4,193,719
_RS20010
Position (kb)
4190
4191
4192 Strain fitness (log2 ratio)
-1
0
1 at 4189.302 kb on - strand, within BWI76_RS19995 at 4189.375 kb on - strand, within BWI76_RS19995 at 4189.976 kb on - strand at 4190.084 kb on + strand at 4190.092 kb on - strand at 4190.611 kb on - strand, within BWI76_RS20000 at 4191.142 kb on - strand, within BWI76_RS20000 at 4191.665 kb on + strand, within BWI76_RS20005 at 4191.715 kb on + strand, within BWI76_RS20005 at 4191.904 kb on + strand, within BWI76_RS20005 at 4191.926 kb on + strand, within BWI76_RS20005 at 4191.957 kb on + strand at 4192.059 kb on + strand at 4192.099 kb on - strand at 4192.114 kb on - strand at 4192.291 kb on - strand at 4192.335 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 4,189,302 - BWI76_RS19995 0.16 +0.0 4,189,375 - BWI76_RS19995 0.25 +0.0 4,189,976 - +0.6 4,190,084 + -0.4 4,190,092 - -0.1 4,190,611 - BWI76_RS20000 0.37 -0.3 4,191,142 - BWI76_RS20000 0.82 -1.2 4,191,665 + BWI76_RS20005 0.38 -0.3 4,191,715 + BWI76_RS20005 0.47 -0.3 4,191,904 + BWI76_RS20005 0.82 +0.4 4,191,926 + BWI76_RS20005 0.86 -0.7 4,191,957 + +0.7 4,192,059 + -1.6 4,192,099 - -0.1 4,192,114 - -0.0 4,192,291 - -0.5 4,192,335 + -0.3
Or see this region's nucleotide sequence