Experiment: LB 0.5x with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11805 and BWI76_RS11810 are separated by 313 nucleotides BWI76_RS11810 and BWI76_RS11815 are separated by 97 nucleotides BWI76_RS11815 and BWI76_RS11820 are separated by 214 nucleotides
BWI76_RS11805: BWI76_RS11805 - hypothetical protein, at 2,483,314 to 2,484,057
_RS11805
BWI76_RS11810: BWI76_RS11810 - outer membrane protein OmpW, at 2,484,371 to 2,485,009
_RS11810
BWI76_RS11815: BWI76_RS11815 - hypothetical protein, at 2,485,107 to 2,485,352
_RS11815
BWI76_RS11820: BWI76_RS11820 - short-chain dehydrogenase/reductase SDR, at 2,485,567 to 2,486,271
_RS11820
Position (kb)
2484
2485
2486 Strain fitness (log2 ratio)
-1
0
1 at 2483.407 kb on - strand, within BWI76_RS11805 at 2483.509 kb on + strand, within BWI76_RS11805 at 2483.919 kb on + strand, within BWI76_RS11805 at 2483.952 kb on - strand, within BWI76_RS11805 at 2484.066 kb on - strand at 2484.120 kb on + strand at 2484.126 kb on - strand at 2484.197 kb on + strand at 2484.323 kb on + strand at 2484.521 kb on - strand, within BWI76_RS11810 at 2484.588 kb on + strand, within BWI76_RS11810 at 2484.801 kb on + strand, within BWI76_RS11810 at 2485.030 kb on + strand at 2485.279 kb on + strand, within BWI76_RS11815 at 2485.498 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 2,483,407 - BWI76_RS11805 0.12 -0.1 2,483,509 + BWI76_RS11805 0.26 -0.7 2,483,919 + BWI76_RS11805 0.81 -0.4 2,483,952 - BWI76_RS11805 0.86 +0.3 2,484,066 - -0.9 2,484,120 + -0.4 2,484,126 - -0.5 2,484,197 + +0.2 2,484,323 + +0.4 2,484,521 - BWI76_RS11810 0.23 -0.9 2,484,588 + BWI76_RS11810 0.34 -1.0 2,484,801 + BWI76_RS11810 0.67 -0.1 2,485,030 + -1.1 2,485,279 + BWI76_RS11815 0.70 +0.3 2,485,498 - +0.3
Or see this region's nucleotide sequence