Experiment: LB Anaerobic with Phenazine ethosulfate 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt actP and yjcH overlap by 4 nucleotides yjcH and acs are separated by 199 nucleotides
b4067: actP - acetate permease (NCBI), at 4,281,276 to 4,282,925
actP
b4068: yjcH - required for glycolate transport, with actP (from data) , at 4,282,922 to 4,283,236
yjcH
b4069: acs - acetyl-coenzyme A synthetase (NCBI), at 4,283,436 to 4,285,394
acs
Position (kb)
4282
4283
4284 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4281.927 kb on - strand, within actP at 4281.934 kb on + strand, within actP at 4282.003 kb on - strand, within actP at 4282.033 kb on - strand, within actP at 4282.072 kb on - strand, within actP at 4282.094 kb on - strand, within actP at 4282.191 kb on - strand, within actP at 4282.191 kb on - strand, within actP at 4282.215 kb on - strand, within actP at 4282.215 kb on - strand, within actP at 4282.412 kb on - strand, within actP at 4282.531 kb on + strand, within actP at 4282.706 kb on - strand, within actP at 4282.718 kb on - strand, within actP at 4282.718 kb on - strand, within actP at 4282.738 kb on - strand, within actP at 4282.738 kb on - strand, within actP at 4282.740 kb on - strand, within actP at 4282.842 kb on + strand at 4282.864 kb on - strand at 4282.873 kb on - strand at 4282.909 kb on + strand at 4282.938 kb on - strand at 4282.990 kb on + strand, within yjcH at 4283.005 kb on + strand, within yjcH at 4283.013 kb on - strand, within yjcH at 4283.013 kb on - strand, within yjcH at 4283.013 kb on - strand, within yjcH at 4283.050 kb on + strand, within yjcH at 4283.121 kb on - strand, within yjcH at 4283.212 kb on - strand at 4283.212 kb on - strand at 4283.218 kb on + strand at 4283.224 kb on - strand at 4283.224 kb on - strand at 4283.291 kb on - strand at 4283.315 kb on - strand at 4283.315 kb on - strand at 4283.395 kb on + strand at 4283.395 kb on + strand at 4283.423 kb on + strand at 4283.423 kb on + strand at 4283.514 kb on + strand at 4283.514 kb on + strand at 4283.595 kb on - strand at 4283.678 kb on - strand, within acs at 4283.678 kb on - strand, within acs at 4283.712 kb on + strand, within acs at 4283.749 kb on - strand, within acs at 4283.808 kb on + strand, within acs at 4283.855 kb on + strand, within acs at 4283.855 kb on + strand, within acs at 4283.865 kb on + strand, within acs at 4283.956 kb on + strand, within acs at 4283.974 kb on - strand, within acs at 4283.981 kb on - strand, within acs at 4283.981 kb on - strand, within acs at 4283.983 kb on + strand, within acs at 4284.007 kb on - strand, within acs at 4284.032 kb on - strand, within acs at 4284.032 kb on - strand, within acs at 4284.215 kb on + strand, within acs at 4284.215 kb on + strand, within acs at 4284.229 kb on - strand, within acs at 4284.233 kb on + strand, within acs
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Phenazine ethosulfate 0.000125 mM remove 4,281,927 - actP b4067 0.39 -0.8 4,281,934 + actP b4067 0.40 +0.7 4,282,003 - actP b4067 0.44 -0.1 4,282,033 - actP b4067 0.46 +0.1 4,282,072 - actP b4067 0.48 +0.0 4,282,094 - actP b4067 0.50 -0.3 4,282,191 - actP b4067 0.55 -0.2 4,282,191 - actP b4067 0.55 +0.3 4,282,215 - actP b4067 0.57 +0.3 4,282,215 - actP b4067 0.57 +0.0 4,282,412 - actP b4067 0.69 -0.0 4,282,531 + actP b4067 0.76 -0.8 4,282,706 - actP b4067 0.87 -0.7 4,282,718 - actP b4067 0.87 +0.8 4,282,718 - actP b4067 0.87 +0.5 4,282,738 - actP b4067 0.89 -0.0 4,282,738 - actP b4067 0.89 -0.6 4,282,740 - actP b4067 0.89 +0.1 4,282,842 + +0.1 4,282,864 - +0.8 4,282,873 - -0.6 4,282,909 + +1.1 4,282,938 - -1.7 4,282,990 + yjcH b4068 0.22 -1.4 4,283,005 + yjcH b4068 0.26 -2.5 4,283,013 - yjcH b4068 0.29 -0.4 4,283,013 - yjcH b4068 0.29 -2.2 4,283,013 - yjcH b4068 0.29 +0.5 4,283,050 + yjcH b4068 0.41 -0.3 4,283,121 - yjcH b4068 0.63 -0.9 4,283,212 - -0.9 4,283,212 - -0.2 4,283,218 + +0.9 4,283,224 - -2.3 4,283,224 - -1.4 4,283,291 - -1.6 4,283,315 - -0.1 4,283,315 - +0.0 4,283,395 + -0.7 4,283,395 + +0.1 4,283,423 + -0.4 4,283,423 + +0.1 4,283,514 + +0.6 4,283,514 + -2.1 4,283,595 - +0.0 4,283,678 - acs b4069 0.12 +0.4 4,283,678 - acs b4069 0.12 +1.4 4,283,712 + acs b4069 0.14 +0.8 4,283,749 - acs b4069 0.16 -0.0 4,283,808 + acs b4069 0.19 -0.9 4,283,855 + acs b4069 0.21 +0.6 4,283,855 + acs b4069 0.21 +0.7 4,283,865 + acs b4069 0.22 -3.0 4,283,956 + acs b4069 0.27 -1.1 4,283,974 - acs b4069 0.27 -0.8 4,283,981 - acs b4069 0.28 +0.4 4,283,981 - acs b4069 0.28 +2.0 4,283,983 + acs b4069 0.28 +1.2 4,284,007 - acs b4069 0.29 -0.0 4,284,032 - acs b4069 0.30 +1.1 4,284,032 - acs b4069 0.30 -2.0 4,284,215 + acs b4069 0.40 -0.1 4,284,215 + acs b4069 0.40 +1.4 4,284,229 - acs b4069 0.40 -2.5 4,284,233 + acs b4069 0.41 +0.4
Or see this region's nucleotide sequence