Experiment: LB Anaerobic with Phenazine ethosulfate 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt garD and sohA are separated by 148 nucleotides sohA and yhaV overlap by 1 nucleotides yhaV and agaR are separated by 54 nucleotides
b3128: garD - (D)-galactarate dehydrogenase (NCBI), at 3,273,304 to 3,274,875
garD
b3129: sohA - predicted regulator (NCBI), at 3,275,024 to 3,275,359
sohA
b3130: yhaV - hypothetical protein (NCBI), at 3,275,359 to 3,275,823
yhaV
b3131: agaR - DNA-binding transcriptional dual regulator (NCBI), at 3,275,878 to 3,276,687
agaR
Position (kb)
3275
3276 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3274.131 kb on - strand, within garD at 3274.152 kb on - strand, within garD at 3274.162 kb on - strand, within garD at 3274.168 kb on + strand, within garD at 3274.168 kb on + strand, within garD at 3274.172 kb on - strand, within garD at 3274.468 kb on - strand, within garD at 3274.508 kb on - strand, within garD at 3274.541 kb on - strand, within garD at 3274.571 kb on + strand, within garD at 3274.619 kb on + strand, within garD at 3274.623 kb on + strand, within garD at 3274.709 kb on + strand, within garD at 3274.836 kb on - strand at 3275.046 kb on + strand at 3275.046 kb on + strand at 3275.049 kb on - strand at 3275.049 kb on - strand at 3275.049 kb on - strand at 3275.051 kb on + strand at 3275.051 kb on + strand at 3275.057 kb on - strand at 3275.059 kb on - strand, within sohA at 3275.063 kb on + strand, within sohA at 3275.098 kb on + strand, within sohA at 3275.108 kb on + strand, within sohA at 3275.108 kb on + strand, within sohA at 3275.118 kb on + strand, within sohA at 3275.118 kb on + strand, within sohA at 3275.318 kb on - strand, within sohA at 3275.318 kb on - strand, within sohA at 3275.392 kb on + strand at 3275.392 kb on + strand at 3275.392 kb on + strand at 3275.400 kb on - strand at 3275.400 kb on - strand at 3275.420 kb on - strand, within yhaV at 3275.427 kb on + strand, within yhaV at 3275.451 kb on + strand, within yhaV at 3275.512 kb on + strand, within yhaV at 3275.536 kb on + strand, within yhaV at 3275.596 kb on + strand, within yhaV at 3275.642 kb on - strand, within yhaV at 3275.642 kb on - strand, within yhaV at 3275.678 kb on + strand, within yhaV at 3275.685 kb on - strand, within yhaV at 3275.685 kb on - strand, within yhaV at 3275.686 kb on - strand, within yhaV at 3275.686 kb on - strand, within yhaV at 3275.690 kb on - strand, within yhaV at 3275.735 kb on + strand, within yhaV at 3275.741 kb on - strand, within yhaV at 3275.781 kb on - strand at 3275.781 kb on - strand at 3276.109 kb on + strand, within agaR at 3276.131 kb on - strand, within agaR at 3276.131 kb on - strand, within agaR at 3276.131 kb on - strand, within agaR at 3276.170 kb on + strand, within agaR at 3276.242 kb on - strand, within agaR at 3276.242 kb on - strand at 3276.270 kb on + strand, within agaR
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Phenazine ethosulfate 0.000125 mM remove 3,274,131 - garD b3128 0.53 -2.7 3,274,152 - garD b3128 0.54 -0.2 3,274,162 - garD b3128 0.55 +0.1 3,274,168 + garD b3128 0.55 +1.4 3,274,168 + garD b3128 0.55 +0.5 3,274,172 - garD b3128 0.55 -0.3 3,274,468 - garD b3128 0.74 -0.7 3,274,508 - garD b3128 0.77 +1.0 3,274,541 - garD b3128 0.79 -0.3 3,274,571 + garD b3128 0.81 -0.3 3,274,619 + garD b3128 0.84 +0.2 3,274,623 + garD b3128 0.84 -0.4 3,274,709 + garD b3128 0.89 +0.4 3,274,836 - -0.0 3,275,046 + +0.5 3,275,046 + +1.5 3,275,049 - -0.2 3,275,049 - +0.1 3,275,049 - -0.2 3,275,051 + +0.4 3,275,051 + +1.9 3,275,057 - -0.4 3,275,059 - sohA b3129 0.10 -1.5 3,275,063 + sohA b3129 0.12 -1.5 3,275,098 + sohA b3129 0.22 -0.5 3,275,108 + sohA b3129 0.25 -2.2 3,275,108 + sohA b3129 0.25 -2.1 3,275,118 + sohA b3129 0.28 -0.3 3,275,118 + sohA b3129 0.28 -0.9 3,275,318 - sohA b3129 0.88 +0.8 3,275,318 - sohA b3129 0.88 +0.9 3,275,392 + -1.9 3,275,392 + -0.1 3,275,392 + -2.4 3,275,400 - -1.3 3,275,400 - -0.1 3,275,420 - yhaV b3130 0.13 +0.9 3,275,427 + yhaV b3130 0.15 +0.6 3,275,451 + yhaV b3130 0.20 -0.7 3,275,512 + yhaV b3130 0.33 +0.5 3,275,536 + yhaV b3130 0.38 -0.1 3,275,596 + yhaV b3130 0.51 +0.4 3,275,642 - yhaV b3130 0.61 +0.6 3,275,642 - yhaV b3130 0.61 -1.1 3,275,678 + yhaV b3130 0.69 -0.2 3,275,685 - yhaV b3130 0.70 -0.3 3,275,685 - yhaV b3130 0.70 -0.2 3,275,686 - yhaV b3130 0.70 -1.4 3,275,686 - yhaV b3130 0.70 -0.1 3,275,690 - yhaV b3130 0.71 +0.0 3,275,735 + yhaV b3130 0.81 +0.9 3,275,741 - yhaV b3130 0.82 +0.5 3,275,781 - -2.0 3,275,781 - +0.6 3,276,109 + agaR b3131 0.29 +0.3 3,276,131 - agaR b3131 0.31 -0.5 3,276,131 - agaR b3131 0.31 -0.4 3,276,131 - agaR b3131 0.31 +1.7 3,276,170 + agaR b3131 0.36 -0.7 3,276,242 - agaR b3131 0.45 -0.4 3,276,242 - +0.3 3,276,270 + agaR b3131 0.48 -1.2
Or see this region's nucleotide sequence