Experiment: LB Anaerobic with Norfloxacin 0.00000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycgE and ycgF are separated by 204 nucleotides ycgF and ycgZ are separated by 313 nucleotides ycgZ and ymgA are separated by 42 nucleotides ymgA and ymgB are separated by 28 nucleotides
b1162: ycgE - predicted DNA-binding transcriptional regulator (NCBI), at 1,212,551 to 1,213,282
ycgE
b1163: ycgF - predicted FAD-binding phosphodiesterase (NCBI), at 1,213,487 to 1,214,698
ycgF
b1164: ycgZ - hypothetical protein (NCBI), at 1,215,012 to 1,215,248
ycgZ
b1165: ymgA - hypothetical protein (NCBI), at 1,215,291 to 1,215,563
ymgA
b1166: ymgB - hypothetical protein (NCBI), at 1,215,592 to 1,215,858
ymgB
Position (kb)
1213
1214
1215 Strain fitness (log2 ratio)
-1
0
1 at 1212.579 kb on - strand at 1212.579 kb on - strand at 1212.596 kb on - strand at 1212.618 kb on + strand at 1212.689 kb on - strand, within ycgE at 1212.689 kb on - strand, within ycgE at 1212.803 kb on + strand, within ycgE at 1212.864 kb on - strand, within ycgE at 1212.892 kb on - strand, within ycgE at 1212.894 kb on + strand, within ycgE at 1212.951 kb on - strand, within ycgE at 1212.954 kb on + strand, within ycgE at 1212.993 kb on + strand, within ycgE at 1213.017 kb on - strand, within ycgE at 1213.051 kb on - strand, within ycgE at 1213.051 kb on - strand, within ycgE at 1213.160 kb on - strand, within ycgE at 1213.171 kb on + strand, within ycgE at 1213.247 kb on + strand at 1213.265 kb on - strand at 1213.265 kb on - strand at 1213.277 kb on - strand at 1213.301 kb on + strand at 1213.314 kb on + strand at 1213.314 kb on + strand at 1213.314 kb on + strand at 1213.314 kb on + strand at 1213.434 kb on - strand at 1213.517 kb on - strand at 1213.581 kb on + strand at 1213.592 kb on + strand at 1213.592 kb on + strand at 1213.594 kb on + strand at 1213.600 kb on - strand at 1213.602 kb on - strand at 1213.612 kb on - strand, within ycgF at 1213.612 kb on - strand, within ycgF at 1213.620 kb on - strand, within ycgF at 1213.758 kb on - strand, within ycgF at 1213.759 kb on - strand, within ycgF at 1213.789 kb on + strand, within ycgF at 1213.789 kb on + strand, within ycgF at 1213.797 kb on - strand, within ycgF at 1213.799 kb on + strand, within ycgF at 1213.799 kb on - strand, within ycgF at 1213.846 kb on - strand, within ycgF at 1213.858 kb on - strand, within ycgF at 1213.902 kb on + strand, within ycgF at 1213.910 kb on - strand, within ycgF at 1213.960 kb on - strand, within ycgF at 1213.993 kb on - strand, within ycgF at 1213.993 kb on - strand, within ycgF at 1214.007 kb on + strand, within ycgF at 1214.067 kb on + strand, within ycgF at 1214.067 kb on + strand, within ycgF at 1214.151 kb on - strand, within ycgF at 1214.190 kb on + strand, within ycgF at 1214.190 kb on + strand, within ycgF at 1214.194 kb on - strand, within ycgF at 1214.203 kb on + strand, within ycgF at 1214.203 kb on + strand, within ycgF at 1214.203 kb on + strand, within ycgF at 1214.257 kb on + strand, within ycgF at 1214.366 kb on + strand, within ycgF at 1214.460 kb on + strand, within ycgF at 1214.460 kb on - strand, within ycgF at 1214.478 kb on + strand, within ycgF at 1214.541 kb on - strand, within ycgF at 1214.575 kb on + strand, within ycgF at 1214.590 kb on - strand at 1214.596 kb on - strand at 1214.612 kb on - strand at 1214.614 kb on + strand at 1214.614 kb on + strand at 1214.617 kb on - strand at 1214.718 kb on - strand at 1214.718 kb on - strand at 1214.730 kb on - strand at 1214.730 kb on - strand at 1214.730 kb on - strand at 1214.789 kb on + strand at 1215.133 kb on + strand, within ycgZ at 1215.188 kb on + strand, within ycgZ at 1215.190 kb on + strand, within ycgZ at 1215.288 kb on + strand at 1215.346 kb on + strand, within ymgA at 1215.347 kb on + strand, within ymgA at 1215.349 kb on + strand, within ymgA
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Norfloxacin 0.00000015625 mM remove 1,212,579 - +1.7 1,212,579 - +0.3 1,212,596 - -0.9 1,212,618 + +0.7 1,212,689 - ycgE b1162 0.19 +1.1 1,212,689 - ycgE b1162 0.19 -0.9 1,212,803 + ycgE b1162 0.34 +0.0 1,212,864 - ycgE b1162 0.43 -0.9 1,212,892 - ycgE b1162 0.47 +0.7 1,212,894 + ycgE b1162 0.47 +1.7 1,212,951 - ycgE b1162 0.55 -0.4 1,212,954 + ycgE b1162 0.55 +0.2 1,212,993 + ycgE b1162 0.60 +1.7 1,213,017 - ycgE b1162 0.64 +0.7 1,213,051 - ycgE b1162 0.68 +0.1 1,213,051 - ycgE b1162 0.68 -0.4 1,213,160 - ycgE b1162 0.83 +0.4 1,213,171 + ycgE b1162 0.85 +0.1 1,213,247 + +0.1 1,213,265 - +1.7 1,213,265 - +1.7 1,213,277 - +1.7 1,213,301 + -1.3 1,213,314 + -0.7 1,213,314 + -1.7 1,213,314 + +0.7 1,213,314 + +0.4 1,213,434 - -1.0 1,213,517 - -0.7 1,213,581 + +1.7 1,213,592 + +1.7 1,213,592 + +1.7 1,213,594 + +0.7 1,213,600 - +1.7 1,213,602 - +1.7 1,213,612 - ycgF b1163 0.10 +0.7 1,213,612 - ycgF b1163 0.10 +1.7 1,213,620 - ycgF b1163 0.11 +1.7 1,213,758 - ycgF b1163 0.22 -0.6 1,213,759 - ycgF b1163 0.22 -1.3 1,213,789 + ycgF b1163 0.25 +0.1 1,213,789 + ycgF b1163 0.25 -0.3 1,213,797 - ycgF b1163 0.26 +1.1 1,213,799 + ycgF b1163 0.26 +0.1 1,213,799 - ycgF b1163 0.26 +0.7 1,213,846 - ycgF b1163 0.30 +0.1 1,213,858 - ycgF b1163 0.31 +1.7 1,213,902 + ycgF b1163 0.34 +0.1 1,213,910 - ycgF b1163 0.35 +0.7 1,213,960 - ycgF b1163 0.39 -0.1 1,213,993 - ycgF b1163 0.42 +0.4 1,213,993 - ycgF b1163 0.42 -0.3 1,214,007 + ycgF b1163 0.43 +1.7 1,214,067 + ycgF b1163 0.48 +0.7 1,214,067 + ycgF b1163 0.48 +0.7 1,214,151 - ycgF b1163 0.55 +0.7 1,214,190 + ycgF b1163 0.58 +0.1 1,214,190 + ycgF b1163 0.58 +0.7 1,214,194 - ycgF b1163 0.58 +1.7 1,214,203 + ycgF b1163 0.59 -1.3 1,214,203 + ycgF b1163 0.59 +0.1 1,214,203 + ycgF b1163 0.59 -0.3 1,214,257 + ycgF b1163 0.64 -0.3 1,214,366 + ycgF b1163 0.73 +1.1 1,214,460 + ycgF b1163 0.80 -0.3 1,214,460 - ycgF b1163 0.80 +0.7 1,214,478 + ycgF b1163 0.82 +0.1 1,214,541 - ycgF b1163 0.87 -0.3 1,214,575 + ycgF b1163 0.90 +1.7 1,214,590 - -0.9 1,214,596 - +0.7 1,214,612 - -0.3 1,214,614 + +0.7 1,214,614 + -1.2 1,214,617 - -0.8 1,214,718 - +0.7 1,214,718 - +1.0 1,214,730 - -0.3 1,214,730 - +0.7 1,214,730 - -0.3 1,214,789 + +1.7 1,215,133 + ycgZ b1164 0.51 +0.3 1,215,188 + ycgZ b1164 0.74 +0.7 1,215,190 + ycgZ b1164 0.75 -1.3 1,215,288 + +1.7 1,215,346 + ymgA b1165 0.20 +1.7 1,215,347 + ymgA b1165 0.21 +1.7 1,215,349 + ymgA b1165 0.21 +0.7
Or see this region's nucleotide sequence