Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08285

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08280 and BWI76_RS08285 are separated by 184 nucleotidesBWI76_RS08285 and BWI76_RS08290 are separated by 32 nucleotides BWI76_RS08280: BWI76_RS08280 - acyl-CoA esterase, at 1,751,986 to 1,752,759 _RS08280 BWI76_RS08285: BWI76_RS08285 - replication initiation regulator SeqA, at 1,752,944 to 1,753,492 _RS08285 BWI76_RS08290: BWI76_RS08290 - phosphoglucomutase, alpha-D-glucose phosphate-specific, at 1,753,525 to 1,755,165 _RS08290 Position (kb) 1752 1753 1754Strain fitness (log2 ratio) -2 -1 0 1at 1752.008 kb on - strandat 1752.163 kb on - strand, within BWI76_RS08280at 1752.589 kb on + strand, within BWI76_RS08280at 1752.605 kb on - strand, within BWI76_RS08280at 1752.857 kb on + strandat 1752.862 kb on + strandat 1752.936 kb on + strandat 1753.237 kb on + strand, within BWI76_RS08285at 1753.371 kb on - strand, within BWI76_RS08285

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
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1,752,008 - -0.0
1,752,163 - BWI76_RS08280 0.23 +0.2
1,752,589 + BWI76_RS08280 0.78 +1.7
1,752,605 - BWI76_RS08280 0.80 +1.0
1,752,857 + -1.8
1,752,862 + +0.3
1,752,936 + +0.8
1,753,237 + BWI76_RS08285 0.53 -2.7
1,753,371 - BWI76_RS08285 0.78 -1.8

Or see this region's nucleotide sequence