Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0923 and Psyr_0924 are separated by 109 nucleotides Psyr_0924 and Psyr_0925 are separated by 8 nucleotides Psyr_0925 and Psyr_0926 overlap by 4 nucleotides
Psyr_0923: Psyr_0923 - hypothetical protein, at 1,050,093 to 1,051,553
_0923
Psyr_0924: Psyr_0924 - Glucose-1-phosphate thymidylyltransferase, at 1,051,663 to 1,052,544
_0924
Psyr_0925: Psyr_0925 - dTDP-4-dehydrorhamnose reductase, at 1,052,553 to 1,053,458
_0925
Psyr_0926: Psyr_0926 - dTDP-glucose 4,6-dehydratase, at 1,053,455 to 1,054,555
_0926
Position (kb) 1051
1052
1053 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1050.822 kb on - strand, within Psyr_0923 at 1050.893 kb on + strand, within Psyr_0923 at 1051.041 kb on - strand, within Psyr_0923 at 1051.097 kb on + strand, within Psyr_0923 at 1051.097 kb on + strand, within Psyr_0923 at 1051.098 kb on - strand, within Psyr_0923 at 1051.101 kb on + strand, within Psyr_0923 at 1051.101 kb on + strand, within Psyr_0923 at 1051.141 kb on + strand, within Psyr_0923 at 1051.142 kb on - strand at 1051.173 kb on + strand, within Psyr_0923 at 1051.234 kb on + strand, within Psyr_0923 at 1051.235 kb on - strand, within Psyr_0923 at 1051.235 kb on - strand, within Psyr_0923 at 1051.235 kb on - strand, within Psyr_0923 at 1051.326 kb on + strand, within Psyr_0923 at 1051.326 kb on + strand, within Psyr_0923 at 1051.326 kb on + strand, within Psyr_0923 at 1051.327 kb on - strand, within Psyr_0923 at 1051.452 kb on - strand at 1051.502 kb on + strand at 1051.502 kb on + strand at 1051.510 kb on + strand at 1051.510 kb on + strand at 1051.510 kb on + strand at 1051.563 kb on + strand at 1051.563 kb on + strand at 1051.563 kb on + strand at 1051.563 kb on + strand at 1051.563 kb on + strand at 1051.563 kb on + strand at 1051.563 kb on + strand at 1051.563 kb on + strand at 1051.563 kb on + strand at 1051.563 kb on + strand at 1051.564 kb on - strand at 1051.564 kb on - strand at 1051.564 kb on - strand at 1051.564 kb on - strand at 1051.564 kb on - strand at 1051.564 kb on - strand at 1051.564 kb on - strand at 1051.564 kb on - strand at 1051.904 kb on - strand, within Psyr_0924 at 1052.013 kb on - strand, within Psyr_0924 at 1052.037 kb on - strand, within Psyr_0924 at 1053.474 kb on + strand at 1053.474 kb on + strand at 1053.474 kb on + strand at 1053.475 kb on - strand at 1053.475 kb on - strand at 1053.475 kb on - strand at 1053.475 kb on - strand at 1053.475 kb on - strand at 1053.498 kb on + strand at 1053.543 kb on + strand at 1053.543 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 1,050,822 - Psyr_0923 0.50 -1.6 1,050,893 + Psyr_0923 0.55 -0.5 1,051,041 - Psyr_0923 0.65 +1.4 1,051,097 + Psyr_0923 0.69 -0.2 1,051,097 + Psyr_0923 0.69 +0.1 1,051,098 - Psyr_0923 0.69 -2.1 1,051,101 + Psyr_0923 0.69 +1.2 1,051,101 + Psyr_0923 0.69 -0.9 1,051,141 + Psyr_0923 0.72 -0.5 1,051,142 - +1.4 1,051,173 + Psyr_0923 0.74 -1.1 1,051,234 + Psyr_0923 0.78 -0.3 1,051,235 - Psyr_0923 0.78 +1.4 1,051,235 - Psyr_0923 0.78 -0.9 1,051,235 - Psyr_0923 0.78 +0.9 1,051,326 + Psyr_0923 0.84 +0.1 1,051,326 + Psyr_0923 0.84 +1.0 1,051,326 + Psyr_0923 0.84 +0.1 1,051,327 - Psyr_0923 0.84 +0.4 1,051,452 - +1.4 1,051,502 + +0.4 1,051,502 + +0.8 1,051,510 + -0.3 1,051,510 + +1.4 1,051,510 + -0.6 1,051,563 + -0.1 1,051,563 + -0.1 1,051,563 + -0.2 1,051,563 + -0.3 1,051,563 + +0.9 1,051,563 + -0.3 1,051,563 + +0.1 1,051,563 + -0.8 1,051,563 + -0.0 1,051,563 + -0.2 1,051,564 - +1.4 1,051,564 - -1.2 1,051,564 - -0.9 1,051,564 - -0.3 1,051,564 - -0.9 1,051,564 - -2.2 1,051,564 - -1.2 1,051,564 - +1.9 1,051,904 - Psyr_0924 0.27 +0.4 1,052,013 - Psyr_0924 0.40 -1.8 1,052,037 - Psyr_0924 0.42 -1.4 1,053,474 + -1.6 1,053,474 + -1.2 1,053,474 + +0.4 1,053,475 - +0.8 1,053,475 - +0.3 1,053,475 - +0.2 1,053,475 - -1.9 1,053,475 - -0.7 1,053,498 + +1.7 1,053,543 + +0.4 1,053,543 + -0.4
Or see this region's nucleotide sequence