Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0487 and Psyr_0488 are separated by 254 nucleotides Psyr_0488 and Psyr_0489 are separated by 46 nucleotides Psyr_0489 and Psyr_0490 are separated by 14 nucleotides
Psyr_0487: Psyr_0487 - glutathione synthase, at 524,260 to 525,219
_0487
Psyr_0488: Psyr_0488 - Response regulator receiver, at 525,474 to 525,884
_0488
Psyr_0489: Psyr_0489 - Response regulator receiver, at 525,931 to 526,296
_0489
Psyr_0490: Psyr_0490 - CheW-like protein, at 526,311 to 526,850
_0490
Position (kb)
525
526 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 524.510 kb on - strand, within Psyr_0487 at 525.107 kb on + strand, within Psyr_0487 at 525.107 kb on + strand, within Psyr_0487 at 525.107 kb on + strand, within Psyr_0487 at 525.107 kb on + strand, within Psyr_0487 at 525.107 kb on + strand, within Psyr_0487 at 525.108 kb on - strand, within Psyr_0487 at 525.108 kb on - strand, within Psyr_0487 at 525.108 kb on - strand, within Psyr_0487 at 525.108 kb on - strand, within Psyr_0487 at 525.227 kb on - strand at 525.360 kb on + strand at 525.360 kb on + strand at 525.360 kb on + strand at 525.637 kb on + strand, within Psyr_0488 at 525.637 kb on + strand, within Psyr_0488 at 525.877 kb on + strand at 525.877 kb on + strand at 525.877 kb on + strand at 525.877 kb on + strand at 525.877 kb on + strand at 525.877 kb on + strand at 525.877 kb on + strand at 525.877 kb on + strand at 525.877 kb on + strand at 525.877 kb on + strand at 525.877 kb on + strand at 525.877 kb on + strand at 525.878 kb on - strand at 525.878 kb on - strand at 525.878 kb on - strand at 525.878 kb on - strand at 525.878 kb on - strand at 525.878 kb on - strand at 525.878 kb on - strand at 525.878 kb on - strand at 525.878 kb on - strand at 525.879 kb on - strand at 525.900 kb on + strand at 525.900 kb on + strand at 525.900 kb on + strand at 525.900 kb on + strand at 525.912 kb on - strand at 525.912 kb on - strand at 525.973 kb on + strand, within Psyr_0489 at 526.156 kb on - strand, within Psyr_0489 at 526.651 kb on + strand, within Psyr_0490 at 526.651 kb on + strand, within Psyr_0490 at 526.651 kb on + strand, within Psyr_0490 at 526.651 kb on + strand, within Psyr_0490 at 526.651 kb on + strand, within Psyr_0490 at 526.651 kb on + strand, within Psyr_0490 at 526.651 kb on + strand, within Psyr_0490 at 526.651 kb on + strand, within Psyr_0490 at 526.652 kb on - strand, within Psyr_0490 at 526.652 kb on - strand, within Psyr_0490 at 526.652 kb on - strand, within Psyr_0490 at 526.652 kb on - strand, within Psyr_0490 at 526.652 kb on - strand, within Psyr_0490 at 526.745 kb on + strand, within Psyr_0490 at 526.745 kb on + strand, within Psyr_0490 at 526.746 kb on - strand, within Psyr_0490 at 526.746 kb on - strand, within Psyr_0490 at 526.773 kb on - strand, within Psyr_0490 at 526.848 kb on + strand at 526.848 kb on + strand at 526.849 kb on - strand at 526.849 kb on - strand at 526.849 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 524,510 - Psyr_0487 0.26 -1.4 525,107 + Psyr_0487 0.88 +0.5 525,107 + Psyr_0487 0.88 -0.5 525,107 + Psyr_0487 0.88 -0.8 525,107 + Psyr_0487 0.88 -1.7 525,107 + Psyr_0487 0.88 -1.3 525,108 - Psyr_0487 0.88 -0.8 525,108 - Psyr_0487 0.88 -0.1 525,108 - Psyr_0487 0.88 -2.5 525,108 - Psyr_0487 0.88 +0.2 525,227 - -2.1 525,360 + -0.6 525,360 + +0.0 525,360 + +1.5 525,637 + Psyr_0488 0.40 -0.3 525,637 + Psyr_0488 0.40 +0.3 525,877 + +0.6 525,877 + -0.2 525,877 + +0.9 525,877 + +0.6 525,877 + -1.0 525,877 + +1.1 525,877 + +1.5 525,877 + -0.8 525,877 + -2.8 525,877 + -0.5 525,877 + +0.4 525,877 + -0.2 525,878 - +0.2 525,878 - -0.2 525,878 - -1.3 525,878 - +0.5 525,878 - -1.5 525,878 - -0.1 525,878 - -0.1 525,878 - -0.6 525,878 - -3.0 525,879 - -0.4 525,900 + -0.8 525,900 + +0.8 525,900 + -0.2 525,900 + -0.8 525,912 - -2.6 525,912 - -1.7 525,973 + Psyr_0489 0.11 -0.8 526,156 - Psyr_0489 0.61 -2.3 526,651 + Psyr_0490 0.63 -0.2 526,651 + Psyr_0490 0.63 -0.2 526,651 + Psyr_0490 0.63 +1.0 526,651 + Psyr_0490 0.63 +0.7 526,651 + Psyr_0490 0.63 +0.9 526,651 + Psyr_0490 0.63 -0.0 526,651 + Psyr_0490 0.63 -2.2 526,651 + Psyr_0490 0.63 -0.5 526,652 - Psyr_0490 0.63 -0.2 526,652 - Psyr_0490 0.63 -2.3 526,652 - Psyr_0490 0.63 +1.8 526,652 - Psyr_0490 0.63 -1.9 526,652 - Psyr_0490 0.63 -3.4 526,745 + Psyr_0490 0.80 +1.5 526,745 + Psyr_0490 0.80 -0.8 526,746 - Psyr_0490 0.81 -0.5 526,746 - Psyr_0490 0.81 -0.5 526,773 - Psyr_0490 0.86 -1.3 526,848 + -2.6 526,848 + -0.5 526,849 - -1.6 526,849 - -1.2 526,849 - +0.3
Or see this region's nucleotide sequence