Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5350 and Pf1N1B4_5351 overlap by 24 nucleotides Pf1N1B4_5351 and Pf1N1B4_5352 overlap by 32 nucleotides Pf1N1B4_5352 and Pf1N1B4_5353 are separated by 516 nucleotides Pf1N1B4_5353 and Pf1N1B4_5354 are separated by 109 nucleotides Pf1N1B4_5354 and Pf1N1B4_5355 are separated by 17 nucleotides Pf1N1B4_5355 and Pf1N1B4_5356 are separated by 360 nucleotides
Pf1N1B4_5350 - hypothetical protein, at 90,270 to 91,034
_5350
Pf1N1B4_5351 - hypothetical protein, at 91,011 to 91,241
_5351
Pf1N1B4_5352 - hypothetical protein, at 91,210 to 91,479
_5352
Pf1N1B4_5353 - hypothetical protein, at 91,996 to 92,163
_5353
Pf1N1B4_5354 - hypothetical protein, at 92,273 to 92,416
_5354
Pf1N1B4_5355 - hypothetical protein, at 92,434 to 92,616
_5355
Pf1N1B4_5356 - hypothetical protein, at 92,977 to 93,222
_5356
Position (kb)
91
92
93 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 91.045 kb on + strand, within Pf1N1B4_5351 at 91.045 kb on + strand, within Pf1N1B4_5351 at 91.045 kb on + strand, within Pf1N1B4_5351 at 91.046 kb on - strand, within Pf1N1B4_5351 at 91.046 kb on - strand, within Pf1N1B4_5351 at 91.046 kb on - strand, within Pf1N1B4_5351 at 91.046 kb on - strand, within Pf1N1B4_5351 at 91.175 kb on + strand, within Pf1N1B4_5351 at 91.176 kb on - strand, within Pf1N1B4_5351 at 91.197 kb on + strand, within Pf1N1B4_5351 at 91.197 kb on + strand, within Pf1N1B4_5351 at 91.197 kb on + strand, within Pf1N1B4_5351 at 91.197 kb on + strand, within Pf1N1B4_5351 at 91.198 kb on - strand, within Pf1N1B4_5351 at 91.211 kb on + strand at 91.211 kb on + strand at 91.212 kb on - strand at 91.249 kb on + strand, within Pf1N1B4_5352 at 91.493 kb on + strand at 91.493 kb on + strand at 91.494 kb on - strand at 91.523 kb on + strand at 91.523 kb on + strand at 91.524 kb on - strand at 91.567 kb on + strand at 91.625 kb on + strand at 91.625 kb on + strand at 91.625 kb on + strand at 91.626 kb on - strand at 91.626 kb on - strand at 91.626 kb on - strand at 91.792 kb on + strand at 91.793 kb on - strand at 91.793 kb on - strand at 91.793 kb on - strand at 91.794 kb on + strand at 91.794 kb on + strand at 91.794 kb on + strand at 91.794 kb on + strand at 91.794 kb on + strand at 91.794 kb on + strand at 91.871 kb on + strand at 91.871 kb on + strand at 91.871 kb on + strand at 91.871 kb on + strand at 91.871 kb on + strand at 91.871 kb on + strand at 91.871 kb on + strand at 91.871 kb on + strand at 91.871 kb on + strand at 91.872 kb on - strand at 91.891 kb on + strand at 91.891 kb on + strand at 91.891 kb on + strand at 91.891 kb on + strand at 91.891 kb on + strand at 91.891 kb on + strand at 91.892 kb on - strand at 91.907 kb on + strand at 91.907 kb on + strand at 91.907 kb on + strand at 91.907 kb on + strand at 91.907 kb on + strand at 91.908 kb on - strand at 91.908 kb on - strand at 91.917 kb on + strand at 91.917 kb on + strand at 91.917 kb on + strand at 91.917 kb on + strand at 91.917 kb on + strand at 91.917 kb on + strand at 91.917 kb on + strand at 91.929 kb on + strand at 91.929 kb on + strand at 91.929 kb on + strand at 91.929 kb on + strand at 91.929 kb on + strand at 91.929 kb on + strand at 91.929 kb on + strand at 91.929 kb on + strand at 91.929 kb on + strand at 91.930 kb on - strand at 91.930 kb on - strand at 91.950 kb on + strand at 91.993 kb on + strand at 91.993 kb on + strand at 91.993 kb on + strand at 91.993 kb on + strand at 91.994 kb on - strand at 92.092 kb on + strand, within Pf1N1B4_5353 at 92.092 kb on + strand, within Pf1N1B4_5353 at 92.092 kb on + strand, within Pf1N1B4_5353 at 92.092 kb on + strand, within Pf1N1B4_5353 at 92.092 kb on + strand, within Pf1N1B4_5353 at 92.092 kb on + strand, within Pf1N1B4_5353 at 92.092 kb on + strand, within Pf1N1B4_5353 at 92.093 kb on - strand, within Pf1N1B4_5353 at 92.093 kb on - strand, within Pf1N1B4_5353 at 92.161 kb on + strand at 92.165 kb on + strand at 92.165 kb on + strand at 92.186 kb on + strand at 92.302 kb on + strand, within Pf1N1B4_5354 at 92.338 kb on + strand, within Pf1N1B4_5354 at 92.338 kb on + strand, within Pf1N1B4_5354 at 92.338 kb on + strand, within Pf1N1B4_5354 at 92.339 kb on - strand, within Pf1N1B4_5354 at 92.349 kb on + strand, within Pf1N1B4_5354 at 92.349 kb on + strand, within Pf1N1B4_5354 at 92.385 kb on + strand, within Pf1N1B4_5354 at 92.443 kb on + strand at 92.443 kb on + strand at 92.496 kb on + strand, within Pf1N1B4_5355 at 92.496 kb on + strand at 92.497 kb on - strand, within Pf1N1B4_5355 at 92.500 kb on + strand, within Pf1N1B4_5355 at 92.500 kb on + strand, within Pf1N1B4_5355 at 92.501 kb on - strand, within Pf1N1B4_5355 at 92.501 kb on - strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.539 kb on + strand, within Pf1N1B4_5355 at 92.541 kb on + strand, within Pf1N1B4_5355 at 92.541 kb on + strand, within Pf1N1B4_5355 at 92.541 kb on + strand, within Pf1N1B4_5355 at 92.626 kb on + strand at 92.626 kb on + strand at 92.658 kb on + strand at 92.658 kb on + strand at 92.677 kb on + strand at 92.677 kb on + strand at 92.677 kb on + strand at 92.678 kb on - strand at 92.678 kb on - strand at 92.871 kb on + strand at 92.871 kb on + strand at 92.872 kb on - strand at 92.872 kb on - strand at 92.934 kb on + strand at 92.934 kb on + strand at 92.934 kb on + strand at 92.934 kb on + strand at 92.934 kb on + strand at 92.935 kb on - strand at 92.935 kb on - strand at 92.935 kb on - strand at 92.935 kb on - strand at 92.935 kb on - strand at 92.935 kb on - strand at 92.935 kb on - strand at 92.935 kb on - strand at 92.935 kb on - strand at 92.937 kb on + strand at 92.937 kb on + strand at 92.937 kb on + strand at 92.937 kb on + strand at 92.937 kb on + strand at 92.937 kb on + strand at 92.937 kb on + strand at 92.937 kb on + strand at 92.937 kb on + strand at 92.938 kb on - strand at 92.938 kb on - strand at 92.938 kb on - strand at 92.939 kb on + strand at 92.939 kb on + strand at 92.939 kb on + strand at 92.955 kb on - strand at 92.955 kb on - strand at 92.955 kb on - strand at 92.966 kb on - strand at 92.995 kb on + strand at 92.996 kb on - strand at 92.996 kb on - strand at 93.001 kb on + strand at 93.001 kb on + strand at 93.036 kb on + strand, within Pf1N1B4_5356 at 93.114 kb on + strand, within Pf1N1B4_5356 at 93.147 kb on + strand, within Pf1N1B4_5356 at 93.147 kb on + strand, within Pf1N1B4_5356
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 91,045 + Pf1N1B4_5351 0.15 +0.1 91,045 + Pf1N1B4_5351 0.15 +1.1 91,045 + Pf1N1B4_5351 0.15 -0.3 91,046 - Pf1N1B4_5351 0.15 +1.7 91,046 - Pf1N1B4_5351 0.15 +0.1 91,046 - Pf1N1B4_5351 0.15 +0.7 91,046 - Pf1N1B4_5351 0.15 +2.1 91,175 + Pf1N1B4_5351 0.71 +0.1 91,176 - Pf1N1B4_5351 0.71 -0.3 91,197 + Pf1N1B4_5351 0.81 -0.3 91,197 + Pf1N1B4_5351 0.81 +0.9 91,197 + Pf1N1B4_5351 0.81 -0.6 91,197 + Pf1N1B4_5351 0.81 -2.1 91,198 - Pf1N1B4_5351 0.81 +0.4 91,211 + +2.5 91,211 + +0.5 91,212 - -0.3 91,249 + Pf1N1B4_5352 0.14 -1.3 91,493 + -2.8 91,493 + -1.0 91,494 - -0.9 91,523 + +0.1 91,523 + +1.0 91,524 - -0.2 91,567 + +0.5 91,625 + -2.0 91,625 + -0.5 91,625 + -0.7 91,626 - +2.6 91,626 - -0.6 91,626 - -0.4 91,792 + +1.1 91,793 - -0.6 91,793 - -0.3 91,793 - -0.1 91,794 + +1.1 91,794 + -0.6 91,794 + +1.7 91,794 + -0.2 91,794 + -0.8 91,794 + +0.0 91,871 + -0.1 91,871 + -0.9 91,871 + +0.3 91,871 + -0.1 91,871 + -0.4 91,871 + +1.9 91,871 + -0.5 91,871 + -2.5 91,871 + +1.4 91,872 - -1.3 91,891 + -0.8 91,891 + +0.2 91,891 + -0.3 91,891 + -0.3 91,891 + -1.0 91,891 + +1.1 91,892 - +0.1 91,907 + -0.7 91,907 + -0.2 91,907 + -0.4 91,907 + -0.3 91,907 + -1.9 91,908 - +1.1 91,908 - -1.1 91,917 + -2.3 91,917 + -0.7 91,917 + +0.1 91,917 + -0.2 91,917 + +1.2 91,917 + +0.8 91,917 + +0.5 91,929 + -1.2 91,929 + -1.3 91,929 + +2.5 91,929 + -0.7 91,929 + -0.8 91,929 + -0.1 91,929 + -2.1 91,929 + +1.1 91,929 + -1.1 91,930 - +0.9 91,930 - +0.9 91,950 + -0.9 91,993 + -1.0 91,993 + +1.1 91,993 + +1.6 91,993 + +0.5 91,994 - -1.1 92,092 + Pf1N1B4_5353 0.57 +1.4 92,092 + Pf1N1B4_5353 0.57 +1.2 92,092 + Pf1N1B4_5353 0.57 +0.3 92,092 + Pf1N1B4_5353 0.57 +0.3 92,092 + Pf1N1B4_5353 0.57 -0.9 92,092 + Pf1N1B4_5353 0.57 -0.7 92,092 + Pf1N1B4_5353 0.57 +1.8 92,093 - Pf1N1B4_5353 0.58 -0.5 92,093 - Pf1N1B4_5353 0.58 +1.1 92,161 + -2.5 92,165 + -1.6 92,165 + -0.3 92,186 + +0.5 92,302 + Pf1N1B4_5354 0.20 -1.7 92,338 + Pf1N1B4_5354 0.45 -1.8 92,338 + Pf1N1B4_5354 0.45 -1.1 92,338 + Pf1N1B4_5354 0.45 -0.7 92,339 - Pf1N1B4_5354 0.46 -0.3 92,349 + Pf1N1B4_5354 0.53 +1.3 92,349 + Pf1N1B4_5354 0.53 +0.1 92,385 + Pf1N1B4_5354 0.78 +1.0 92,443 + -2.1 92,443 + -2.5 92,496 + Pf1N1B4_5355 0.34 +0.6 92,496 + +1.5 92,497 - Pf1N1B4_5355 0.34 +1.5 92,500 + Pf1N1B4_5355 0.36 -1.9 92,500 + Pf1N1B4_5355 0.36 -0.4 92,501 - Pf1N1B4_5355 0.37 +0.6 92,501 - Pf1N1B4_5355 0.37 +1.1 92,539 + Pf1N1B4_5355 0.57 +1.9 92,539 + Pf1N1B4_5355 0.57 -2.4 92,539 + Pf1N1B4_5355 0.57 -1.1 92,539 + Pf1N1B4_5355 0.57 +0.1 92,539 + Pf1N1B4_5355 0.57 +1.8 92,539 + Pf1N1B4_5355 0.57 -1.9 92,539 + Pf1N1B4_5355 0.57 -0.3 92,539 + Pf1N1B4_5355 0.57 +0.2 92,539 + Pf1N1B4_5355 0.57 +1.9 92,539 + Pf1N1B4_5355 0.57 +0.0 92,539 + Pf1N1B4_5355 0.57 +3.4 92,541 + Pf1N1B4_5355 0.58 +0.6 92,541 + Pf1N1B4_5355 0.58 +1.0 92,541 + Pf1N1B4_5355 0.58 -1.3 92,626 + -0.7 92,626 + -0.9 92,658 + -2.0 92,658 + -3.0 92,677 + +2.1 92,677 + +0.4 92,677 + -2.0 92,678 - -0.7 92,678 - -0.5 92,871 + +0.2 92,871 + +0.5 92,872 - -1.0 92,872 - +1.1 92,934 + +0.8 92,934 + +0.6 92,934 + +0.4 92,934 + +3.8 92,934 + +0.2 92,935 - -1.6 92,935 - -0.9 92,935 - -1.0 92,935 - -1.0 92,935 - +1.1 92,935 - +1.1 92,935 - -0.9 92,935 - +0.2 92,935 - +0.7 92,937 + -1.7 92,937 + -1.2 92,937 + +0.3 92,937 + +1.1 92,937 + -1.1 92,937 + -1.1 92,937 + +0.1 92,937 + +1.5 92,937 + +0.6 92,938 - -2.2 92,938 - +1.3 92,938 - +0.2 92,939 + +0.7 92,939 + +4.5 92,939 + -1.2 92,955 - -1.2 92,955 - +0.3 92,955 - +0.1 92,966 - -0.9 92,995 + -1.7 92,996 - +0.2 92,996 - -2.0 93,001 + -1.3 93,001 + -1.5 93,036 + Pf1N1B4_5356 0.24 -0.1 93,114 + Pf1N1B4_5356 0.56 -0.7 93,147 + Pf1N1B4_5356 0.69 -2.9 93,147 + Pf1N1B4_5356 0.69 +0.6
Or see this region's nucleotide sequence