Experiment: Nitrate reduction, replicate C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4563 and Pf1N1B4_4564 are separated by 52 nucleotides Pf1N1B4_4564 and Pf1N1B4_4565 are separated by 407 nucleotides Pf1N1B4_4565 and Pf1N1B4_4566 are separated by 119 nucleotides
Pf1N1B4_4563 - Sulfate transporter, at 5,034,249 to 5,035,925
_4563
Pf1N1B4_4564 - Aconitate hydratase 2 (EC 4.2.1.3) @ 2-methylisocitrate dehydratase (EC 4.2.1.99), at 5,035,978 to 5,038,578
_4564
Pf1N1B4_4565 - FIG00955280: hypothetical protein, at 5,038,986 to 5,039,456
_4565
Pf1N1B4_4566 - Universal stress protein family 7, at 5,039,576 to 5,040,439
_4566
Position (kb)
5035
5036
5037
5038
5039 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5034.988 kb on + strand, within Pf1N1B4_4563 at 5035.029 kb on + strand, within Pf1N1B4_4563 at 5035.269 kb on + strand, within Pf1N1B4_4563 at 5035.269 kb on + strand, within Pf1N1B4_4563 at 5035.356 kb on + strand, within Pf1N1B4_4563 at 5035.530 kb on + strand, within Pf1N1B4_4563 at 5035.530 kb on + strand, within Pf1N1B4_4563 at 5035.923 kb on + strand at 5039.415 kb on + strand at 5039.415 kb on + strand at 5039.415 kb on + strand at 5039.415 kb on + strand at 5039.454 kb on + strand at 5039.558 kb on + strand at 5039.558 kb on + strand at 5039.559 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate C remove 5,034,988 + Pf1N1B4_4563 0.44 -1.2 5,035,029 + Pf1N1B4_4563 0.47 -1.0 5,035,269 + Pf1N1B4_4563 0.61 +0.8 5,035,269 + Pf1N1B4_4563 0.61 -0.4 5,035,356 + Pf1N1B4_4563 0.66 -1.5 5,035,530 + Pf1N1B4_4563 0.76 -0.0 5,035,530 + Pf1N1B4_4563 0.76 +0.6 5,035,923 + +0.2 5,039,415 + -2.9 5,039,415 + +0.1 5,039,415 + +1.3 5,039,415 + +1.1 5,039,454 + +1.6 5,039,558 + +0.8 5,039,558 + +0.4 5,039,559 - +1.6
Or see this region's nucleotide sequence