Experiment: Tween 20 (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1214 and Psest_1215 are separated by 114 nucleotides Psest_1215 and Psest_1216 are separated by 205 nucleotides Psest_1216 and Psest_1217 are separated by 21 nucleotides
Psest_1214: Psest_1214 - Cytosine deaminase and related metal-dependent hydrolases, at 1,319,824 to 1,321,179
_1214
Psest_1215: Psest_1215 - Short-chain dehydrogenase involved in D-alanine esterification of lipoteichoic acid and wall teichoic acid (D-alanine transfer protein), at 1,321,294 to 1,321,743
_1215
Psest_1216: Psest_1216 - ATPase, P-type (transporting), HAD superfamily, subfamily IC, at 1,321,949 to 1,324,663
_1216
Psest_1217: Psest_1217 - Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein, at 1,324,685 to 1,326,145
_1217
Position (kb)
1321
1322
1323
1324
1325 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1321.226 kb on + strand at 1321.227 kb on - strand at 1321.285 kb on + strand at 1321.286 kb on - strand at 1321.286 kb on - strand at 1321.286 kb on - strand at 1321.286 kb on - strand at 1321.436 kb on + strand, within Psest_1215 at 1321.436 kb on + strand, within Psest_1215 at 1321.436 kb on + strand, within Psest_1215 at 1321.437 kb on - strand, within Psest_1215 at 1321.437 kb on - strand, within Psest_1215 at 1321.880 kb on + strand at 1321.881 kb on - strand at 1321.892 kb on + strand at 1321.892 kb on + strand at 1321.892 kb on + strand at 1321.892 kb on + strand at 1321.893 kb on - strand at 1321.893 kb on - strand at 1321.893 kb on - strand at 1321.893 kb on - strand at 1321.899 kb on + strand at 1321.900 kb on - strand at 1321.900 kb on - strand at 1322.136 kb on + strand at 1322.136 kb on + strand at 1322.137 kb on - strand at 1322.137 kb on - strand at 1322.137 kb on - strand at 1322.137 kb on - strand at 1322.137 kb on - strand at 1322.153 kb on + strand at 1322.154 kb on - strand at 1322.206 kb on + strand at 1322.206 kb on + strand at 1322.229 kb on + strand, within Psest_1216 at 1322.229 kb on + strand, within Psest_1216 at 1322.230 kb on - strand, within Psest_1216 at 1322.230 kb on - strand, within Psest_1216 at 1322.862 kb on + strand, within Psest_1216 at 1322.863 kb on - strand, within Psest_1216 at 1323.387 kb on - strand, within Psest_1216 at 1323.550 kb on + strand, within Psest_1216 at 1323.550 kb on + strand, within Psest_1216 at 1323.551 kb on - strand, within Psest_1216 at 1324.315 kb on + strand, within Psest_1216 at 1324.315 kb on + strand, within Psest_1216 at 1324.315 kb on + strand, within Psest_1216 at 1324.316 kb on - strand, within Psest_1216 at 1324.319 kb on + strand, within Psest_1216 at 1324.319 kb on + strand, within Psest_1216 at 1324.319 kb on + strand, within Psest_1216 at 1324.319 kb on + strand, within Psest_1216 at 1324.319 kb on + strand, within Psest_1216 at 1324.319 kb on + strand, within Psest_1216 at 1324.319 kb on + strand, within Psest_1216 at 1324.319 kb on + strand, within Psest_1216 at 1324.319 kb on + strand, within Psest_1216 at 1324.319 kb on + strand, within Psest_1216 at 1324.320 kb on - strand, within Psest_1216 at 1324.320 kb on - strand, within Psest_1216 at 1324.378 kb on + strand, within Psest_1216 at 1324.378 kb on + strand, within Psest_1216 at 1324.378 kb on + strand, within Psest_1216 at 1324.378 kb on + strand, within Psest_1216 at 1324.378 kb on + strand, within Psest_1216 at 1324.379 kb on - strand, within Psest_1216 at 1324.379 kb on - strand, within Psest_1216 at 1324.403 kb on + strand at 1324.403 kb on + strand at 1324.403 kb on + strand at 1324.403 kb on + strand at 1324.403 kb on + strand at 1324.403 kb on + strand at 1324.404 kb on - strand at 1324.404 kb on - strand at 1324.520 kb on + strand at 1324.520 kb on + strand at 1324.521 kb on - strand at 1324.578 kb on + strand at 1324.722 kb on + strand at 1324.722 kb on + strand at 1324.722 kb on + strand at 1324.722 kb on + strand at 1324.723 kb on - strand at 1324.723 kb on - strand at 1324.723 kb on - strand at 1324.723 kb on - strand at 1324.723 kb on - strand at 1324.792 kb on + strand at 1324.792 kb on + strand at 1324.792 kb on + strand at 1324.803 kb on + strand at 1324.806 kb on + strand at 1324.875 kb on + strand, within Psest_1217 at 1324.876 kb on - strand, within Psest_1217 at 1324.876 kb on - strand, within Psest_1217 at 1324.995 kb on + strand, within Psest_1217 at 1325.070 kb on + strand, within Psest_1217 at 1325.071 kb on - strand, within Psest_1217 at 1325.332 kb on - strand, within Psest_1217 at 1325.358 kb on + strand, within Psest_1217 at 1325.514 kb on + strand, within Psest_1217 at 1325.514 kb on + strand, within Psest_1217 at 1325.514 kb on + strand, within Psest_1217 at 1325.515 kb on - strand, within Psest_1217
Per-strain Table
Position Strand Gene LocusTag Fraction Tween 20 (C) remove 1,321,226 + -0.1 1,321,227 - +0.0 1,321,285 + +0.5 1,321,286 - -0.2 1,321,286 - -0.3 1,321,286 - -0.3 1,321,286 - +0.2 1,321,436 + Psest_1215 0.32 -1.8 1,321,436 + Psest_1215 0.32 +2.5 1,321,436 + Psest_1215 0.32 +0.3 1,321,437 - Psest_1215 0.32 -0.5 1,321,437 - Psest_1215 0.32 -1.1 1,321,880 + -2.6 1,321,881 - +0.1 1,321,892 + +0.5 1,321,892 + -0.9 1,321,892 + -0.2 1,321,892 + +0.5 1,321,893 - -0.9 1,321,893 - -0.1 1,321,893 - -1.3 1,321,893 - +0.1 1,321,899 + -0.2 1,321,900 - +0.9 1,321,900 - +0.1 1,322,136 + -0.1 1,322,136 + -0.4 1,322,137 - -0.5 1,322,137 - +0.2 1,322,137 - +0.8 1,322,137 - +0.9 1,322,137 - -0.0 1,322,153 + -0.8 1,322,154 - -0.3 1,322,206 + +0.1 1,322,206 + -0.2 1,322,229 + Psest_1216 0.10 -1.3 1,322,229 + Psest_1216 0.10 -0.6 1,322,230 - Psest_1216 0.10 -1.0 1,322,230 - Psest_1216 0.10 +0.5 1,322,862 + Psest_1216 0.34 -0.7 1,322,863 - Psest_1216 0.34 -1.0 1,323,387 - Psest_1216 0.53 -0.2 1,323,550 + Psest_1216 0.59 -1.0 1,323,550 + Psest_1216 0.59 -2.2 1,323,551 - Psest_1216 0.59 -2.0 1,324,315 + Psest_1216 0.87 +0.2 1,324,315 + Psest_1216 0.87 -1.9 1,324,315 + Psest_1216 0.87 -0.4 1,324,316 - Psest_1216 0.87 -0.5 1,324,319 + Psest_1216 0.87 +0.4 1,324,319 + Psest_1216 0.87 -1.4 1,324,319 + Psest_1216 0.87 -0.3 1,324,319 + Psest_1216 0.87 +0.0 1,324,319 + Psest_1216 0.87 -0.3 1,324,319 + Psest_1216 0.87 -1.0 1,324,319 + Psest_1216 0.87 +0.0 1,324,319 + Psest_1216 0.87 -0.6 1,324,319 + Psest_1216 0.87 -1.4 1,324,319 + Psest_1216 0.87 +0.3 1,324,320 - Psest_1216 0.87 +0.2 1,324,320 - Psest_1216 0.87 -0.8 1,324,378 + Psest_1216 0.89 +0.2 1,324,378 + Psest_1216 0.89 +0.0 1,324,378 + Psest_1216 0.89 -0.2 1,324,378 + Psest_1216 0.89 -0.0 1,324,378 + Psest_1216 0.89 +0.1 1,324,379 - Psest_1216 0.90 +1.2 1,324,379 - Psest_1216 0.90 +0.6 1,324,403 + -0.0 1,324,403 + -1.5 1,324,403 + +0.5 1,324,403 + +0.1 1,324,403 + -0.5 1,324,403 + -1.1 1,324,404 - -0.8 1,324,404 - +0.0 1,324,520 + -0.0 1,324,520 + +0.4 1,324,521 - +0.9 1,324,578 + +1.2 1,324,722 + -0.5 1,324,722 + -1.2 1,324,722 + -0.9 1,324,722 + -0.1 1,324,723 - -0.3 1,324,723 - +0.5 1,324,723 - +0.2 1,324,723 - -0.9 1,324,723 - -0.9 1,324,792 + +0.3 1,324,792 + +0.6 1,324,792 + -0.1 1,324,803 + -0.5 1,324,806 + -0.0 1,324,875 + Psest_1217 0.13 +0.3 1,324,876 - Psest_1217 0.13 -0.2 1,324,876 - Psest_1217 0.13 -0.6 1,324,995 + Psest_1217 0.21 +0.1 1,325,070 + Psest_1217 0.26 +1.0 1,325,071 - Psest_1217 0.26 -0.1 1,325,332 - Psest_1217 0.44 +0.6 1,325,358 + Psest_1217 0.46 +1.0 1,325,514 + Psest_1217 0.57 -0.1 1,325,514 + Psest_1217 0.57 -0.8 1,325,514 + Psest_1217 0.57 -0.2 1,325,515 - Psest_1217 0.57 -0.4
Or see this region's nucleotide sequence