SEED Subsystem: Valine degradation in Pseudomonas fluorescens FW300-N2E3

Experiments:

Role Gene Name L-Isoleucine (C)  
⨯
3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) AO353_00510 -0.7
  AO353_01155 -0.2
3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31) AO353_05985 -1.4
  AO353_22635 -0.1
3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4) AO353_25665 +0.1
Branched-chain acyl-CoA dehydrogenase (EC 1.3.99.12) No genes
Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4) AO353_15575 +0.1
  AO353_26635 -3.1
Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4) AO353_15580 +0.2
  AO353_26640 -3.9
Branched-chain amino acid aminotransferase (EC 2.6.1.42) AO353_26655 -3.1
Butyrate kinase (EC 2.7.2.7) No genes
Butyryl-CoA dehydrogenase (EC 1.3.99.2) AO353_00670 +0.2
  AO353_01500 -0.0
  AO353_15135 -0.1
  AO353_15225 -0.1
  AO353_24835 +0.3
  AO353_25670 -0.2
  AO353_25680 -4.3
  AO353_28600 -0.2
Dihydrolipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (EC 2.3.1.168) AO353_15585 -1.1
  AO353_26645 -3.1
Dihydrolipoamide dehydrogenase of branched-chain alpha-keto acid dehydrogenase (EC 1.8.1.4) AO353_26650 -4.2
Enoyl-CoA hydratase (EC 4.2.1.17) AO353_00510 -0.7
  AO353_03780 -0.1
  AO353_17555 -0.3
  AO353_19125 N.D.
  AO353_23055 +0.3
  AO353_24830 -0.1
Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) AO353_25675 N.D.
  AO353_26540 -0.2
Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (EC 2.3.1.-) No genes
Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27) AO353_05990 -0.5
  AO353_06080 -0.2
Predicted transcriptional regulator of leucine degradation pathway, MerR family No genes
Probable acyl-CoA dehydrogenase (EC 1.3.99.3) AO353_25690 N.D.
Transcriptional regulator BkdR of isoleucine and valine catabolism operon AO353_26630 -3.1
phosphotransbutyrylase (EC 2.3.1.19) No genes

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