SEED Subsystem: Methionine Biosynthesis in Pseudomonas fluorescens FW300-N1B4

Experiments:

Role Gene Name nitrogen source nitrite 0.5mM  
⨯
5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) Pf1N1B4_2384 -2.0
5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13) Pf1N1B4_5661 -0.0
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC 2.1.1.14) Pf1N1B4_783 -0.1
Adenosylhomocysteinase (EC 3.3.1.1) Pf1N1B4_2385 N.D.
Archaeal S-adenosylmethionine synthetase (EC 2.5.1.6) No genes
Betaine--homocysteine S-methyltransferase (EC 2.1.1.5) No genes
Cystathionine beta-lyase (EC 4.4.1.8) Pf1N1B4_4430 -0.3
Cystathionine beta-lyase, type II (EC 4.4.1.8) No genes
Cystathionine beta-synthase (EC 4.2.1.22) Pf1N1B4_4889 +0.3
Cystathionine gamma-lyase (EC 4.4.1.1) Pf1N1B4_4890 -0.0
Cystathionine gamma-synthase (EC 2.5.1.48) No genes
Cysteine synthase (EC 2.5.1.47) Pf1N1B4_3203 -0.0
  Pf1N1B4_3374 +0.0
Duplicated ATPase component MtsB of energizing module of methionine-regulated ECF transporter No genes
Homocysteine S-methyltransferase (EC 2.1.1.10) Pf1N1B4_2014 +0.3
Homoserine O-acetyltransferase (EC 2.3.1.31) Pf1N1B4_2337 -1.7
  Pf1N1B4_2338 -2.3
Homoserine O-succinyltransferase (EC 2.3.1.46) No genes
Homoserine dehydrogenase (EC 1.1.1.3) Pf1N1B4_3013 N.D.
Homoserine kinase (EC 2.7.1.39) Pf1N1B4_1873 N.D.
  Pf1N1B4_3813 -0.1
Methionine ABC transporter ATP-binding protein Pf1N1B4_1702 N.D.
  Pf1N1B4_1866 +0.6
  Pf1N1B4_2642 -0.0
Methionine ABC transporter permease protein Pf1N1B4_1703 -0.0
  Pf1N1B4_1865 -0.2
Methionine ABC transporter substrate-binding protein Pf1N1B4_1701 -0.2
  Pf1N1B4_1714 -0.4
  Pf1N1B4_1864 -0.0
Methionine repressor MetJ No genes
Methionine synthase activation domain (EC 2.1.1.13) No genes
Methionine transporter MetT No genes
O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) Pf1N1B4_3963 +0.0
  Pf1N1B4_4893 +0.3
O-succinylhomoserine sulfhydrylase (EC 2.5.1.48) No genes
Predicted regulator of methionine metabolism, ArsR family No genes
S-adenosylhomocysteine nucleosidase (EC 3.2.2.9) No genes
S-adenosylmethionine synthetase (EC 2.5.1.6) Pf1N1B4_2395 N.D.
S-ribosylhomocysteine lyase (EC 4.4.1.21) No genes
SAM-dependent methlytransferase UbiG No genes
SAM-dependent methlytransferase YrrT No genes
Serine acetyltransferase (EC 2.3.1.30) Pf1N1B4_1690 +0.1
  Pf1N1B4_867 +0.0
Substrate-specific component MtsA of methionine-regulated ECF transporter No genes
Transcriptional activator MetR Pf1N1B4_784 -1.7
Transmembrane component MtsC of energizing module of methionine-regulated ECF transporter No genes

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