MetaCyc Pathway: glycine betaine degradation III in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes Cadmium chloride hemipentahydrate 0.000015625 M
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glycine betaine + NADH + dioxygen + H+→N,N-dimethylglycine + formaldehyde + NAD+ + H2O (EC 1.14.13.251)
Pf6N2E2_4713 +0.0
Pf6N2E2_4714 +0.1
N,N-dimethylglycine + 2 an oxidized ferredoxin [iron-sulfur] cluster + H2O→sarcosine + formaldehyde + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.5.7.3)
Pf6N2E2_4702 -0.2
sarcosine + 2 an oxidized ferredoxin [iron-sulfur] cluster + H2O→glycine + formaldehyde + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.5.7.3)
Pf6N2E2_4702 -0.2
Glycine hydroxymethyltransferase (in reverse):
glycine + a 5,10-methylenetetrahydrofolate + H2O→L-serine + a tetrahydrofolate (EC 2.1.2.1)
Pf6N2E2_4717 +0.2
Pf6N2E2_5082 N.D.
Pf6N2E2_5083 -0.0
Pf6N2E2_5295 -0.2
L-serine ammonia-lyase:
L-serine→2-aminoprop-2-enoate + H2O (EC 4.3.1.17)
Pf6N2E2_4685 +0.0
Pf6N2E2_5557 -1.5
Tryptophanase:
2-aminoprop-2-enoate→2-iminopropanoate (spontaneous) (EC 4.1.99.1; 4.1.99.2; 4.3.1.13; 4.3.1.17; 4.3.1.18; 4.3.3.8; 4.4.1.1; 4.4.1.13; 4.4.1.15; 4.4.1.25; 4.4.1.28; 4.4.1.35; 4.5.1.2)
Pf6N2E2_2047 -0.1
Pf6N2E2_3918 -0.0
Pf6N2E2_4685 +0.0
Pf6N2E2_5557 -1.5
Pf6N2E2_5896 -0.0
2-iminopropanoate + H2O→pyruvate + ammonium (spontaneous) (EC 3.5.99.10; 4.1.99.1; 4.1.99.2; 4.3.1.13; 4.3.1.17; 4.3.1.18; 4.3.3.8; 4.4.1.1; 4.4.1.13; 4.4.1.15; 4.4.1.25; 4.4.1.28; 4.4.1.35; 4.5.1.2)
Pf6N2E2_2047 -0.1
Pf6N2E2_3918 -0.0
Pf6N2E2_4360 -1.0
Pf6N2E2_4685 +0.0
Pf6N2E2_5557 -1.5
Pf6N2E2_5896 -0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information