MetaCyc Pathway: L-glutamate degradation V (via hydroxyglutarate) in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes L-Leucine (C)
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Glutamate dehydrogenase:
L-glutamate + NAD+ + H2O→2-oxoglutarate + ammonium + NADH + H+ (EC 1.4.1.2)
Pf6N2E2_634 -2.2
2-oxoglutarate + NADH + H+→(R)-2-hydroxyglutarate + NAD+ (EC 1.1.1.399)
Pf6N2E2_3932 -4.3
Glutaconate CoA-transferase:
(R)-2-hydroxyglutarate + acetyl-CoA→(R)-2-hydroxyglutaryl-CoA + acetate (EC 2.8.3.12)
Pf6N2E2_2836 +0.1
Pf6N2E2_2837 -0.1
(R)-2-hydroxyglutaryl-CoA→(E)-glutaconyl-CoA + H2O (EC 4.2.1.167)
No genes
(E)-glutaconyl-CoA[in] + Na+[in] + H+[in]→crotonyl-CoA[in] + CO2[in] + Na+[out] (EC 7.2.4.5)
No genes
crotonyl-CoA + H2O→(S)-3-hydroxybutanoyl-CoA (EC 4.2.1.150)
Pf6N2E2_1013 -0.1
Pf6N2E2_1046 -0.3
Pf6N2E2_1834 +0.4
Pf6N2E2_1835 +0.1
Pf6N2E2_1840 +0.5
Pf6N2E2_1922 -0.4
Pf6N2E2_1934 -0.2
Pf6N2E2_1935 -0.0
Pf6N2E2_2290 N.D.
Pf6N2E2_2773 +0.2
Pf6N2E2_3894 -0.1
Pf6N2E2_5493 -0.1
crotonyl-CoA[cytosol] + 2 NADH[cytosol] + 2 an oxidized ferredoxin [iron-sulfur] cluster[cytosol]→butanoyl-CoA[cytosol] + 2 NAD+[cytosol] + 2 a reduced ferredoxin [iron-sulfur] cluster[cytosol] (EC 1.3.1.109)
No genes
Propionate CoA-transferase:
butanoyl-CoA + acetate→acetyl-CoA + butanoate (EC 2.8.3.1; 2.8.3.8)
No genes
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxybutanoyl-CoA + NAD+→acetoacetyl-CoA + NADH + H+ (EC 1.1.1.35)
Pf6N2E2_1722 -0.3
Pf6N2E2_1833 -0.6
Pf6N2E2_1850 -0.1
Pf6N2E2_1922 -0.4
Pf6N2E2_2290 N.D.
Pf6N2E2_3894 -0.1
Pf6N2E2_4690 -0.2
Pf6N2E2_6000 -0.2
Acetyl-CoA C-acyltransferase (in reverse):
acetoacetyl-CoA + coenzyme A→2 acetyl-CoA (EC 2.3.1.16; 2.3.1.9)
Pf6N2E2_1145 N.D.
Pf6N2E2_1675 +0.1
Pf6N2E2_1836 -0.3
Pf6N2E2_1851 -0.1
Pf6N2E2_1923 +0.1
Pf6N2E2_2113 -0.2
Pf6N2E2_2289 N.D.
Pf6N2E2_2539 +0.0
Pf6N2E2_2835 -0.2
Pf6N2E2_3492 +0.1
Pf6N2E2_5999 -0.4

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information