MetaCyc Pathway: cutin biosynthesis in Cupriavidus basilensis FW507-4G11

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Reactions and Genes R2A with Cobalt chloride 0.3 mM
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Palmitoyl-CoA hydrolase:
palmitoyl-CoA + H2O→palmitate + coenzyme A + H+
(EC 3.1.2.2)
RR42_RS10830 -0.1
Palmitoyl-CoA hydrolase:
oleoyl-CoA + H2O→oleate + coenzyme A + H+
(EC 3.1.2.2)
RR42_RS10830 -0.1
palmitate + dioxygen + a reduced [NADPH-hemoprotein reductase]→16-hydroxypalmitate + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.80)
No genes
oleate + dioxygen + a reduced [NADPH-hemoprotein reductase]→18-hydroxyoleate + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.80)
No genes
oleate + a hydroperoxy-fatty-acyl-[lipid]→9,10-epoxystearate + a hydroxy-fatty-acyl-[lipid] (EC 1.11.2.3)
No genes
18-hydroxyoleate + a hydroperoxy-fatty-acyl-[lipid]→9,10-epoxy-18-hydroxystearate + a hydroxy-fatty-acyl-[lipid] (EC 1.11.2.3)
No genes
Long-chain-fatty-acid--CoA ligase:
16-hydroxypalmitate + ATP + coenzyme A→16-hydroxypalmitoyl-CoA + AMP + diphosphate
(EC 6.2.1.3)
RR42_RS01625 +0.0
RR42_RS18150 +2.7
RR42_RS18540 -0.0
RR42_RS25195 +0.1
RR42_RS26915 +0.0
RR42_RS28980 +0.2
RR42_RS29035 +0.0
RR42_RS29460 -0.0
RR42_RS29475 -0.2
RR42_RS36095 +0.1
RR42_RS36385 -0.1
RR42_RS36505 +0.1
18-hydroxyoleate + NADP+→18-oxo-oleate + NADPH + H+
No genes
16-hydroxypalmitate + NADP+→16-oxo-palmitate + NADPH + H+
No genes
9,10-epoxystearate + dioxygen + a reduced [NADPH-hemoprotein reductase]→9,10-epoxy-18-hydroxystearate + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.80)
No genes
9,10-epoxystearate + H2O→(9R,10S)-dihydroxystearate
No genes
16-hydroxypalmitoyl-CoA + NADPH + dioxygen + H+→10,16-dihydroxypalmitoyl-CoA + NADP+ + H2O
No genes
9,10-epoxy-18-hydroxystearate + H2O→9,10,18-trihydroxystearate
No genes
18-oxo-oleate + NADP+ + H2O→α,ω-9Z-octadecenedioate + NADPH + 2 H+
No genes
16-oxo-palmitate + NADP+ + H2O→hexadecanedioate + NADPH + 2 H+
No genes
(9R,10S)-dihydroxystearate + dioxygen + a reduced [NADPH-hemoprotein reductase]→9,10,18-trihydroxystearate + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.80)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information