MetaCyc Pathway: pyruvate fermentation to hexanol (engineered) in Pseudomonas fluorescens FW300-N2C3

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Reactions and Genes L-Lysine (C)
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Pyruvate synthase:
pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
No genes
Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
AO356_02325 -1.8
AO356_03560 -0.2
AO356_04930 -0.6
AO356_08090 -0.3
AO356_20395 +0.5
AO356_21640 -4.3
AO356_26350 +0.9
AO356_30300 -0.1
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
AO356_02330 N.D.
AO356_10120 -0.0
AO356_13845 +0.0
AO356_20400 +0.0
AO356_29995 -0.3
AO356_30295 +0.2
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
AO356_02330 N.D.
AO356_04640 +0.0
AO356_10120 -0.0
AO356_12440 +0.0
AO356_17825 +0.2
AO356_26645 +0.1
AO356_30295 +0.2
AO356_30355 -0.3
AO356_30360 -0.2
Trans-2-enoyl-CoA reductase (NAD(+)) (in reverse):
crotonyl-CoA + NADH + H+→butanoyl-CoA + NAD+ (EC 1.3.1.44)
AO356_17045 -0.1
AO356_20030 -0.1
AO356_26355 -0.1
AO356_26365 -0.2
AO356_29315 +0.4
Acetyl-CoA C-acyltransferase:
acetyl-CoA + butanoyl-CoA→3-oxohexanoyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
AO356_02325 -1.8
AO356_03560 -0.2
AO356_04930 -0.6
AO356_08090 -0.3
AO356_20395 +0.5
AO356_21640 -4.3
AO356_26350 +0.9
AO356_30300 -0.1
3-hydroxyacyl-CoA dehydrogenase (in reverse):
3-oxohexanoyl-CoA + NADH + H+→(S)-3-hydroxyhexanoyl-CoA + NAD+ (EC 1.1.1.35)
AO356_02330 N.D.
AO356_10120 -0.0
AO356_13845 +0.0
AO356_20400 +0.0
AO356_30295 +0.2
Long-chain-enoyl-CoA hydratase:
(S)-3-hydroxyhexanoyl-CoA→(2E)-hex-2-enoyl-CoA + H2O (EC 4.2.1.74)
AO356_02330 N.D.
AO356_04640 +0.0
AO356_10120 -0.0
AO356_12440 +0.0
AO356_17825 +0.2
AO356_26645 +0.1
AO356_30295 +0.2
AO356_30355 -0.3
AO356_30360 -0.2
Trans-2-enoyl-CoA reductase (NAD(+)) (in reverse):
(2E)-hex-2-enoyl-CoA + NADH + H+→hexanoyl-CoA + NAD+ (EC 1.3.1.44)
No genes
hexanoyl-CoA + NAD(P)H + H+→1-hexanal + coenzyme A + NAD(P)+
No genes
1-hexanal + NADH + H+→hexan-1-ol + NAD+
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information