MetaCyc Pathway: L-isoleucine degradation III (oxidative Stickland reaction) in Methanococcus maripaludis JJ

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Reactions and Genes Formate pooled triplicate planktonic cells
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Branched-chain-amino-acid transaminase:
L-isoleucine + 2-oxoglutarate→(3S)-3-methyl-2-oxopentanoate + L-glutamate (EC 2.6.1.42)
MMJJ_RS04725 -0.0
3-methyl-2-oxobutanoate dehydrogenase (ferredoxin):
(3S)-3-methyl-2-oxopentanoate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→(2S)-2-methylbutanoyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.7)
MMJJ_RS07805 -1.9
MMJJ_RS07810 -2.2
MMJJ_RS07815 -2.1
(2S)-2-methylbutanoyl-CoA + H2O→2-methylbutanoate + coenzyme A + H+
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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information