MetaCyc Pathway: biotin biosynthesis I in Variovorax sp. OAS795

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Reactions and Genes D-Arabinose (C)
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a malonyl-[acp] + S-adenosyl-L-methionine→a malonyl-[acp] methyl ester + S-adenosyl-L-homocysteine (EC 2.1.1.197)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase II:
a malonyl-[acp] + a malonyl-[acp] methyl ester + H+→a 3-oxoglutaryl-[acp] methyl ester + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
ABID97_RS02835 -0.1
ABID97_RS03825 +0.1
ABID97_RS08320 N.D.
ABID97_RS11000 -0.1
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxoglutaryl-[acp] methyl ester + NADPH + H+→a (3R)-3-hydroxyglutaryl-[acp] methyl ester + NADP+ (EC 1.1.1.100)
ABID97_RS01400 -0.0
ABID97_RS03055 +0.1
ABID97_RS03260 N.D.
ABID97_RS04295 -0.5
ABID97_RS05575 +0.1
ABID97_RS05830 +2.0
ABID97_RS08310 N.D.
ABID97_RS08870 N.D.
ABID97_RS10320 -0.3
ABID97_RS11250 +0.1
ABID97_RS11585 N.D.
ABID97_RS11630 -0.1
ABID97_RS14425 +0.0
ABID97_RS14760 +0.6
ABID97_RS15765 +0.2
ABID97_RS16045 N.D.
ABID97_RS16765 +0.4
ABID97_RS17320 +0.0
ABID97_RS18630 -0.1
ABID97_RS18660 -0.0
ABID97_RS18980 +0.1
ABID97_RS18985 -0.3
ABID97_RS18990 +0.6
ABID97_RS22670 N.D.
ABID97_RS24025 -0.2
ABID97_RS25995 N.D.
ABID97_RS26005 N.D.
ABID97_RS26430 N.D.
ABID97_RS27295 N.D.
ABID97_RS28245 -0.8
ABID97_RS28425 -0.1
ABID97_RS28765 N.D.
ABID97_RS29505 -0.5
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxyglutaryl-[acp] methyl ester→a (2E)-enoylglutaryl-[acp] methyl ester + H2O (EC 4.2.1.59)
ABID97_RS17115 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-enoylglutaryl-[acp] methyl ester + NADH + H+→a glutaryl-[acp] methyl ester + NAD+ (EC 1.3.1.9)
ABID97_RS16525 N.D.
ABID97_RS19675 -0.2
ABID97_RS27875 N.D.
Beta-ketoacyl-acyl-carrier-protein synthase II:
a glutaryl-[acp] methyl ester + a malonyl-[acp] + H+→a 3-oxopimeloyl-[acp] methyl ester + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
ABID97_RS02835 -0.1
ABID97_RS03825 +0.1
ABID97_RS08320 N.D.
ABID97_RS11000 -0.1
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxopimeloyl-[acp] methyl ester + NADPH + H+→a (3R)-3-hydroxypimeloyl-[acp] methyl ester + NADP+ (EC 1.1.1.100)
ABID97_RS01400 -0.0
ABID97_RS03055 +0.1
ABID97_RS03260 N.D.
ABID97_RS04295 -0.5
ABID97_RS05575 +0.1
ABID97_RS05830 +2.0
ABID97_RS08310 N.D.
ABID97_RS08870 N.D.
ABID97_RS10320 -0.3
ABID97_RS11250 +0.1
ABID97_RS11585 N.D.
ABID97_RS11630 -0.1
ABID97_RS14425 +0.0
ABID97_RS14760 +0.6
ABID97_RS15765 +0.2
ABID97_RS16045 N.D.
ABID97_RS16765 +0.4
ABID97_RS17320 +0.0
ABID97_RS18630 -0.1
ABID97_RS18660 -0.0
ABID97_RS18980 +0.1
ABID97_RS18985 -0.3
ABID97_RS18990 +0.6
ABID97_RS22670 N.D.
ABID97_RS24025 -0.2
ABID97_RS25995 N.D.
ABID97_RS26005 N.D.
ABID97_RS26430 N.D.
ABID97_RS27295 N.D.
ABID97_RS28245 -0.8
ABID97_RS28425 -0.1
ABID97_RS28765 N.D.
ABID97_RS29505 -0.5
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxypimeloyl-[acp] methyl ester→a (2E)-enoylpimeloyl-[acp] methyl ester + H2O (EC 4.2.1.59)
ABID97_RS17115 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-enoylpimeloyl-[acp] methyl ester + NADH + H+→a pimeloyl-[acp] methyl ester + NAD+ (EC 1.3.1.9)
ABID97_RS16525 N.D.
ABID97_RS19675 -0.2
ABID97_RS27875 N.D.
a pimeloyl-[acp] methyl ester + H2O→a pimeloyl-[acp] + methanol + H+ (EC 3.1.1.85)
No genes
8-amino-7-oxononanoate synthase:
a pimeloyl-[acp] + L-alanine + H+→(8S)-8-amino-7-oxononanoate + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.47)
ABID97_RS16260 -0.2
Adenosylmethionine--8-amino-7-oxononanoate transaminase:
(8S)-8-amino-7-oxononanoate + S-adenosyl-L-methionine→(7R,8S)-diaminononanoate + S-adenosyl-4-methylsulfanyl-2-oxobutanoate (EC 2.6.1.62)
ABID97_RS16265 -0.1
ABID97_RS25930 +0.0
ABID97_RS26630 -0.2
Dethiobiotin synthase:
(7R,8S)-diaminononanoate + ATP + CO2→dethiobiotin + ADP + 3 H+ + phosphate (EC 6.3.3.3)
ABID97_RS16255 N.D.
Biotin synthase:
dethiobiotin + H+ + 2 a reduced [2Fe-2S] ferredoxin + S-adenosyl-L-methionine + a sulfurated [sulfur carrier]→4,5-secobiotin + 5'-deoxyadenosine + L-methionine + 2 an oxidized [2Fe-2S] ferredoxin + an unsulfurated [sulfur carrier] (EC 2.8.1.6)
ABID97_RS13955 -0.3
Biotin synthase:
4,5-secobiotin + S-adenosyl-L-methionine→biotin + 5'-deoxyadenosine + L-methionine + H+ (EC 2.8.1.6)
ABID97_RS13955 -0.3

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information