MetaCyc Pathway: reductive TCA cycle I in Klebsiella michiganensis M5al

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Reactions and Genes D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days
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2-oxoglutarate synthase (in reverse):
succinyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→2-oxoglutarate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.2.7.3)
No genes
Isocitrate dehydrogenase (NADP(+)) (in reverse):
2-oxoglutarate + CO2 + NADPH→D-threo-isocitrate + NADP+ (EC 1.1.1.42)
BWI76_RS11330 N.D.
Aconitate hydratase (in reverse):
D-threo-isocitrate→cis-aconitate + H2O (EC 4.2.1.3)
BWI76_RS04910 N.D.
BWI76_RS11940 +0.1
Aconitate hydratase (in reverse):
cis-aconitate + H2O→citrate (EC 4.2.1.3)
BWI76_RS04910 N.D.
BWI76_RS11940 +0.1
ATP citrate synthase (in reverse):
citrate + ATP + coenzyme A→acetyl-CoA + oxaloacetate + ADP + phosphate (EC 2.3.3.8)
No genes
Pyruvate synthase (in reverse):
acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
BWI76_RS12730 -0.0
BWI76_RS18805 -1.4
Pyruvate carboxylase:
pyruvate + ATP + hydrogen carbonate→oxaloacetate + ADP + H+ + phosphate (EC 6.4.1.1)
No genes
Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
BWI76_RS13830 -0.1
BWI76_RS16775 -0.5
BWI76_RS25420 +0.4
Fumarate hydratase:
(S)-malate→fumarate + H2O (EC 4.2.1.2)
BWI76_RS08450 -0.0
BWI76_RS16115 +0.2
BWI76_RS16270 -0.1
BWI76_RS16275 -0.5
BWI76_RS16955 -0.7
Succinate dehydrogenase (ubiquinone):
fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
BWI76_RS02440 -0.7
BWI76_RS02445 -0.5
BWI76_RS02450 -1.0
BWI76_RS02455 -0.8
Succinate--CoA ligase (ADP-forming):
succinate + ATP + coenzyme A→succinyl-CoA + ADP + phosphate (EC 6.2.1.5)
BWI76_RS08425 +0.9
BWI76_RS08430 +0.9

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information