MetaCyc Pathway: putrescine degradation II in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
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Glutamate--putrescine ligase:
putrescine + ATP + L-glutamate→γ-glutamyl-L-putrescine + ADP + H+ + phosphate (EC 6.3.1.11)
Pf6N2E2_4509 -4.0
γ-glutamyl-L-putrescine + dioxygen + H2O→4-(γ-L-glutamylamino)butanal + ammonium + hydrogen peroxide (EC 1.4.3.M3)
Pf6N2E2_4388 -0.3
Pf6N2E2_80 -2.7
4-(γ-L-glutamylamino)butanal + NAD(P)+ + H2O→4-(γ-L-glutamylamino)butanoate + NAD(P)H + 2 H+ (EC 1.2.1.99)
Pf6N2E2_4383 -2.6
Gamma-glutamyl-gamma-aminobutyrate hydrolase:
4-(γ-L-glutamylamino)butanoate + H2O→4-aminobutanoate + L-glutamate (EC 3.5.1.94)
Pf6N2E2_4510 -0.4

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information