MetaCyc Pathway: superpathway of fermentation (Chlamydomonas reinhardtii) in Escherichia coli BW25113

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Reactions and Genes LB Anaerobic with Spiramycin 0.000125 mM
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Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
b0823 (ybiW) -0.1
b0824 (ybiY) -0.1
b0902 (pflA) -5.2
b0903 (pflB) -4.3
b2579 (yfiD) -0.5
b3951 (pflD) -0.1
b3952 (pflC) +0.0
b3114 (tdcE) +0.1
Pyruvate decarboxylase:
pyruvate + H+→acetaldehyde + CO2 (EC 4.1.1.1)
No genes
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
b0351 (mhpF) +0.0
b1241 (adhE) -1.3
b2455 (eutE) -0.5
Pyruvate synthase:
pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
b1378 (ydbK) +0.2
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
b0325 (yahK) +0.1
b0356 (frmA) +0.0
b1241 (adhE) -1.3
b1478 (adhP) +0.6
b3253 (yhdH) -1.3
b4269 (yjgB) +0.3
b3589 (yiaY) +0.2
D-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(R)-lactate + NAD+ (EC 1.1.1.28)
b1380 (ldhA) +0.2
b2133 (dld) +0.1
Ferredoxin hydrogenase (in reverse):
2 a reduced ferredoxin [iron-sulfur] cluster + 2 H+→H2 + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.12.7.2)
b2483 (hyfC) -0.1
b2719 (hycG) +0.3
b2720 (hycF) -0.1
b2721 (hycE) +0.3
b2722 (hycD) +0.3
b2723 (hycC) +0.3
b2724 (hycB) +0.5
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
b2297 (pta) -0.3
b2458 (eutI) -0.3
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
b1849 (purT) +0.1
b2296 (ackA) N.D.
b2460 (eutQ) +0.2
b2461 (eutP) +0.0
b3115 (tdcD) +0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information