| Orth | Species | Gene | Name | Description | Fitness | %Id | Cov |
| | Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.399.1 | | 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) | insig. | 100 | 100 |
| o | Variovorax sp. SCN45 | GFF4178 | | 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) | insig. | 75 | 94 |
| o | Rhizobium sp. OAE497 | ABIE40_RS20900 | | methylenetetrahydrofolate reductase | insig. | 36 | 91 |
| o | Agrobacterium fabrum C58 | Atu1764 | | methylenetetrahydrofolate reductase | sig. | 38 | 86 |
| o | Phaeobacter inhibens DSM 17395 | PGA1_c16050 | | 5,10-methylenetetrahydrofolate reductase | strong | 31 | 99 |
| | Agrobacterium fabrum C58 | Atu1418 | | methylenetetrahydrofolate reductase | sig. | 31 | 95 |
| o | Sinorhizobium meliloti 1021 | SMa2143 | | 5,10-methylenetetrahydrofolate reductase | insig. | 30 | 91 |
| o | Dinoroseobacter shibae DFL-12 | Dshi_1675 | | MetF protein-like protein (RefSeq) | strong | 32 | 93 |
| | Ralstonia solanacearum PSI07 | RPSI07_RS23495 | | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 29 | 77 |
| | Rhizobium sp. OAE497 | ABIE40_RS11950 | | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 32 | 44 |
| | Ralstonia solanacearum GMI1000 | RS_RS00460 | | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 29 | 77 |
| | Ralstonia solanacearum IBSBF1503 | RALBFv3_RS09875 | | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 29 | 77 |
| | Ralstonia solanacearum UW163 | UW163_RS03705 | | methylenetetrahydrofolate reductase [NAD(P)H] | insig. | 29 | 77 |
| | Pseudomonas sp. S08-1 | OH686_10820 | | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 28 | 73 |
| | Desulfovibrio vulgaris Miyazaki F | DvMF_3034 | | 5,10-methylenetetrahydrofolate reductase (RefSeq) | no data | 28 | 59 |
| | Dechlorosoma suillum PS | Dsui_0640 | | 5,10-methylenetetrahydrofolate reductase | no data | 29 | 73 |
| | Agrobacterium fabrum C58 | Atu2123 | | methylenetetrahydrofolate reductase | strong | 31 | 44 |
| | Sinorhizobium meliloti 1021 | SMc01843 | | 5,10-methylenetetrahydrofolate reductase | strong | 30 | 51 |
| | Xanthobacter sp. DMC5 | GFF4147 | | 5,10-methylenetetrahydrofolate reductase | no data | 26 | 73 |
| | Desulfovibrio vulgaris Hildenborough JW710 | DVU0997 | metF | 5,10-methylenetetrahydrofolate reductase (TIGR) | strong | 30 | 58 |
| | Pseudomonas fluorescens FW300-N1B4 | Pf1N1B4_2384 | | 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) | strong | 30 | 63 |
| | Pseudomonas fluorescens FW300-N2E2 | Pf6N2E2_4638 | | 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) | strong | 28 | 63 |
| | Ralstonia sp. UNC404CL21Col | ABZR87_RS04865 | | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 28 | 73 |
| | Cupriavidus basilensis FW507-4G11 | RR42_RS01385 | | 5,10-methylenetetrahydrofolate reductase | strong | 27 | 73 |
| | Pseudomonas sp. RS175 | PFR28_04202 | | 5,10-methylenetetrahydrofolate reductase | strong | 30 | 63 |
| | Pseudomonas fluorescens SBW25 | PFLU_RS28165 | | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 29 | 63 |
| | Pseudomonas fluorescens SBW25-INTG | PFLU_RS28165 | | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 29 | 63 |
| | Pseudomonas sp. DMC3 | GFF171 | | 5,10-methylenetetrahydrofolate reductase | strong | 29 | 63 |
| | Pseudomonas stutzeri RCH2 | Psest_0313 | | 5,10-methylenetetrahydrofolate reductase, prokaryotic form | strong | 28 | 71 |
| | Pseudomonas sp. SVBP6 | COO64_RS17485 | metF | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 29 | 63 |
| | Pseudomonas syringae pv. syringae B728a | Psyr_0459 | | 5,10-methylenetetrahydrofolate reductase | sig. | 27 | 63 |
| | Pseudomonas syringae pv. syringae B728a ΔmexB | Psyr_0459 | | 5,10-methylenetetrahydrofolate reductase | insig. | 27 | 63 |
| | Pseudomonas fluorescens FW300-N2C3 | AO356_13605 | | 5,10-methylenetetrahydrofolate reductase | strong | 28 | 63 |
| | Pseudomonas lactucae CFBP13502 | GEMAOFIL_05433 | | 5,10-methylenetetrahydrofolate reductase | strong | 30 | 63 |
| | Pseudomonas simiae WCS417 | PS417_26510 | | 5,10-methylenetetrahydrofolate reductase | strong | 30 | 63 |
| | Methylophilus sp. DMC18 | GFF1492 | | 5,10-methylenetetrahydrofolate reductase | no data | 26 | 88 |
| | Pseudomonas fluorescens FW300-N2E3 | AO353_08015 | | 5,10-methylenetetrahydrofolate reductase | strong | 31 | 63 |
| | Azospirillum brasilense Sp245 | AZOBR_RS24245 | | 5 10-methylenetetrahydrofolate reductase | insig. | 36 | 33 |
| | Castellaniella sp019104865 MT123 | ABCV34_RS13550 | | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 30 | 57 |
| | Azospirillum sp. SherDot2 | MPMX19_00635 | | 5,10-methylenetetrahydrofolate reductase | strong | 29 | 65 |
| | Sphingobium sp. HT1-2 | GFF217 | | 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) | strong | 32 | 50 |
| | Pseudomonas fluorescens GW456-L13 | PfGW456L13_1050 | | 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) | strong | 28 | 63 |
| | Marinobacter adhaerens HP15 | HP15_2917 | | 5,10-methylenetetrahydrofolate reductase-like protein | strong | 27 | 63 |
| | Pseudomonas aeruginosa PA14 | IKLFDK_23270 | | methylenetetrahydrofolate reductase [NAD(P)H] | insig. | 28 | 63 |
| | Azospirillum brasilense Sp245 | AZOBR_RS09540 | | 5 10-methylenetetrahydrofolate reductase | cofit | 27 | 65 |
| | Alteromonas macleodii MIT1002 | MIT1002_03670 | | 5,10-methylenetetrahydrofolate reductase | strong | 31 | 53 |
| | Sphingomonas koreensis DSMZ 15582 | Ga0059261_0992 | | 5,10-methylenetetrahydrofolate reductase, prokaryotic form | strong | 30 | 43 |
| | Pseudomonas segetis P6 | MPMX49_00645 | | 5,10-methylenetetrahydrofolate reductase | strong | 28 | 63 |
| | Hydrogenophaga sp. GW460-11-11-14-LB1 | GFF1692 | | 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) | no data | 26 | 79 |
| | Rahnella sp. WP5 | EX31_RS14205 | | methylenetetrahydrofolate reductase | strong | 30 | 57 |
| | Paraburkholderia graminis OAS925 | ABIE53_000061 | | methylenetetrahydrofolate reductase (NADPH) | strong | 27 | 75 |
| | Pseudomonas putida KT2440 | PP_4977 | | 5,10-methylenetetrahydrofolate reductase | strong | 26 | 63 |
| | Paraburkholderia bryophila 376MFSha3.1 | H281DRAFT_05801 | | 5,10-methylenetetrahydrofolate reductase (NAD(P)) | strong | 26 | 74 |
| | Pseudomonas sp. BP01 | JOY50_RS09810 | metF | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 26 | 63 |
| | Dickeya dadantii 3937 | DDA3937_RS00960 | | methylenetetrahydrofolate reductase | insig. | 31 | 53 |
| | Dickeya dianthicola ME23 | DZA65_RS21460 | | methylenetetrahydrofolate reductase | cofit | 30 | 53 |
| | Caulobacter crescentus NA1000 | CCNA_02224 | | methylenetetrahydrofolate reductase | strong | 29 | 46 |
| | Caulobacter crescentus NA1000 Δfur | CCNA_02224 | | methylenetetrahydrofolate reductase | cofit | 29 | 46 |
| | Herbaspirillum seropedicae SmR1 | HSERO_RS21425 | | 5,10-methylenetetrahydrofolate reductase | strong | 26 | 70 |
| | Burkholderia phytofirmans PsJN | BPHYT_RS18705 | | 5,10-methylenetetrahydrofolate reductase | insig. | 26 | 64 |
| | Xanthomonas campestris pv. campestris strain 8004 | Xcc-8004.4336.1 | | 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) | strong | 26 | 76 |
| | Pantoea agglomerans CFBP13505 P0401 | PagCFBP13505_RS18450 | | methylenetetrahydrofolate reductase | strong | 31 | 53 |
| | Dickeya dianthicola 67-19 | HGI48_RS20415 | | methylenetetrahydrofolate reductase | insig. | 30 | 53 |
| | Lysobacter sp. OAE881 | ABIE51_RS16490 | | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 28 | 54 |
| | Rhodospirillum rubrum S1H | Rru_A1530 | | 5,10-methylenetetrahydrofolate reductase (NCBI) | strong | 25 | 74 |
| | Pantoea sp. MT58 | IAI47_00890 | | methylenetetrahydrofolate reductase | strong | 30 | 53 |
| | Rhodopseudomonas palustris CGA009 | TX73_019175 | | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 39 | 24 |
| | Paraburkholderia sabiae LMG 24235 | QEN71_RS28715 | | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 28 | 74 |
| | Pectobacterium carotovorum WPP14 | HER17_RS00950 | | methylenetetrahydrofolate reductase | insig. | 30 | 53 |
| | Serratia liquefaciens MT49 | IAI46_24530 | | methylenetetrahydrofolate reductase | cofit | 28 | 57 |
| | Brevundimonas sp. GW460-12-10-14-LB2 | A4249_RS07925 | | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 33 | 33 |
| | Magnetospirillum magneticum AMB-1 | AMB_RS02230 | | methylenetetrahydrofolate reductase | no data | 30 | 45 |
| | Dyella japonica UNC79MFTsu3.2 | ABZR86_RS11570 | | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 29 | 43 |
| | Klebsiella michiganensis M5al | BWI76_RS00805 | | 5,10-methylenetetrahydrofolate reductase | strong | 28 | 53 |
| | Rhodanobacter sp000427505 FW510-R12 | LRK53_RS16505 | | methylenetetrahydrofolate reductase [NAD(P)H] | strong | 27 | 53 |